|
|
|
|
|
| Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab |
| HOU Xin, HUANG Feng, ZHANG Tian-yuan, XU Jian-guo, Hyde D Kevin , LI Hong-ye |
1、Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2、Zhejiang Citrus Research Institute, Taizhou 318020, P.R.China
3、Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand |
|
|
|
摘要 Two scab diseases are currently recognized on citrus: citrus scab, caused by Elsinoë fawcettii, and sweet orange scab, caused by E. australis. Although these pathogens are economically important, there is no molecular data on these species in China. Here we use internal transcribed spacer sequence data to report on host-specificity and genetic relationships among 46 isolates collected from the main citrus varieties grown across China. All strains isolated were E. fawcettii. Based on pathogenicity testing on 9 different citrus species, isolates were divided into 11 pathotypes (SM, FBHR, SJCR, SPOJCR, SR, SOJG, SPOJC, SRGC, Lemon and two unnamed pathotypes). SM is a new pathotype, and two isolates did not fit into any of the known pathotypes of E. fawcettii. Inter-simple sequence repeat (ISSR-PCR) assays separated the E. fawcettii isolates into 10 subgroups; the groupings basically corresponded to the pathogenicity test.
Abstract Two scab diseases are currently recognized on citrus: citrus scab, caused by Elsinoë fawcettii, and sweet orange scab, caused by E. australis. Although these pathogens are economically important, there is no molecular data on these species in China. Here we use internal transcribed spacer sequence data to report on host-specificity and genetic relationships among 46 isolates collected from the main citrus varieties grown across China. All strains isolated were E. fawcettii. Based on pathogenicity testing on 9 different citrus species, isolates were divided into 11 pathotypes (SM, FBHR, SJCR, SPOJCR, SR, SOJG, SPOJC, SRGC, Lemon and two unnamed pathotypes). SM is a new pathotype, and two isolates did not fit into any of the known pathotypes of E. fawcettii. Inter-simple sequence repeat (ISSR-PCR) assays separated the E. fawcettii isolates into 10 subgroups; the groupings basically corresponded to the pathogenicity test.
|
|
Received: 08 April 2013
Accepted:
|
| Fund: This work was supported by the China Agriculture Research System (CARS-27) and the Technology Application Research Program for Public Interest of Zhejiang Province, China. |
|
Corresponding Authors:
LI Hong-ye, E-mail: hyli@zju.edu.cn
E-mail: hyli@zju.edu.cn
|
| About author: HOU Xin, E-mail: houxin.sdau@163.com |
Cite this article:
HOU Xin, HUANG Feng, ZHANG Tian-yuan, XU Jian-guo, Hyde D Kevin , LI Hong-ye.
2014.
Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab. Journal of Integrative Agriculture, 13(6): 1293-1302.
|
| Annamalai P, Ishii H, Lalithakumari D, Revathi R. 1995.Polymerase chain reaction and its applications in fungaldisease diagnosis. Zeitschrift für Pflanzenkrankheiten undPflanzenschutz, 102, 91-104Bayraktar H, Dolar F, Maden S. 2008. Use of RAPD and ISSRmarkers in detection of genetic variation and populationstructure among Fusarium oxysporum f. sp. ciceris isolateson chickpea in Turkey. Journal of Phytopathology, 156,146-154Bitancourt A A, Jenkins A E. 1936a. Elsinoë fawcettii, theperfect stage of the citrus scab fungus. Phytopathology,26, 393-395Bitancourt A A, Jenkins A E. 1936b. Perfect stage of the sweetorange fruit scab fungus. Mycologia, 28, 489-492Bornet B, Branchard M. 2001. Nonanchored inter simplesequence repeat (ISSR) markers: Reproducible and specifictools for genome fingerprinting. Plant Molecular BiologyReporter, 19, 209-215van Burik J A H, Schreckhise R, White T, Bowden R,Myerson D. 1998. Comparison of six extraction techniquesfor isolation of DNA from filamentous fungi. MedicalMycology, 36, 299-303Chung K R. 2011. Elsinoë fawcettii and Elsinoë australis: Thefungal pathogens causing citrus scab. Molecular PlantPathology, 12, 123-135Hyun J W, Peres N A, Yi S Y, Timmer L W, Kim K S, KwonH M, Lim H C. 2007. Development of PCR assays for theidentification of species and pathotypes of Elsinoë causingscab on citrus. Plant Disease, 91, 865-870Hyun J W, Timmer L, Lee S C, Yun S H, Ko S W, Kim K S.2001. Pathological characterization and molecular analysisof Elsinoë isolates causing scab diseases of citrus in Jejuisland in Korea. Plant Disease, 85, 1013-1017Hyun J W, Yi S H, MacKenzie S J, Timmer L W, Kim K S,Kang S K, Kwon H M, Lim H C. 2009. Pathotypes andgenetic relationship of worldwide collections of Elsinoëspp. causing scab diseases of citrus. Phytopathology, 99,721-728Jenkins A E. 1925. The citrus scab fungus. Phytopathology,15, 99-104Jenkins A E. 1936. Australian citrus scab caused by Sphacelomafawcettii scabiosa. Phytopathology, 26, 195-197Larkin M, Blackshields G, Brown N, Chenna R, McGettiganP, McWilliam H, Valentin F, Wallace I, Wilm A, Lopez R.2007. Clustal W and Clustal X version 2.0. Bioinformatics,23, 2947-2948Menzies J, Bakkeren G, Matheson F, Procunier J, Woods S.2003. Use of inter-simple sequence repeats and amplifiedfragment length polymorphisms to analyze geneticrelationships among small grain-infecting species ofUstilago. Phytopathology, 93, 167-175Nelson R J, Baraoidan M R, Cruz C M V, Yap I V, Leach J E, Mew T W, Leung H. 1994. Relationship betweenphylogeny and pathotype for the bacterial blight pathogenof rice. Applied and Environmental Microbiology, 60,3275-3283Rohlf F. 2001. NTSYS-PC, numerical taxonomy andmultivariate análisis system. Version 2.1. Exeter Software,Setauket, New York.Sivanesan A, Critchett C. 1974a. Elsinoë australis. In: CMIDescriptions of Pathogenic Fungi and Bacteria No. 438.CAB International, Wallingford, UK.Sivanesan A, Critchett C. 1974b. Elsinoë fawcettii. In: CMIDescriptions of Pathogenic Fungi and Bacteria No. 438.CAB International, Wallingford, UK.Sivanesan A, Critchett C. 1974c. Sphaceloma fawcettii var.scabiosa In: CMI Descriptions of Pathogenic Fungi andBacteria No. 438. CAB International, Wallingford, UK.Swofford D L. 2002. Phylogenetic analysis using parsimony.Version 4b10. Sinauer Associates, Sunderland, MA.Tan M, Timmer L, Broadbent P, Priest M, Cain P. 1996.Differentiation by molecular analysis of Elsinoë spp.causing scab diseases of citrus and its epidemiologicalimplications. Phytopathology, 86, 1039-1044Timmer L, Priest M, Broadbent P, Tan M. 1996. Morphologicaland pathological characterization of species of Elsinoëcausing scab diseases of citrus. Phytopathology, 86,1032-1038Timmer L W, Garnsey S M, Graham J H. 2000. Compendiumof Citrus Diseases. American Phytopathological Society,USA.Whiteside J. 1978. Pathogenicity of two biotypes of Elsinoëfawcettii to sweet orange and some other cultivars.Phytopathology, 68, 1128-1131Zietkiewicz E, Rafalski A, Labuda D. 1994. Genomefingerprinting by simple sequence repeat (SSR)-anchoredpolymerase chain reaction amplification. Genomics, 20,176-183 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
