Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

doi:10.1016/S2095-3119(13)60356-1

Journal of Integrative Agriculture

2014, Vol. 13

Issue (1): 40-53 DOI: 10.1016/S2095-3119(13)60356-1

Crop Genetics · Breeding · Germplasm Resources

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Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

LI Feng-qi, PENG Jun-hua

1.Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, P.R.China
2.University of Chinese Academy of Sciences, Beijing 100049, P.R.China
3.Department of Soil and Crop Sciences, Colorado State University, CO 80526-1170, USA

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摘要 English grain aphid (EGA) is a destructive insect pest of wheat. To identify the loci associated with EGA resistance and tolerance, 70 bread wheat accessions mainly from central Asia were evaluated for EGA resistance and tolerance traits at two locations, and genotyped with 51 SSR markers. Totally, three accessions showed high or moderate levels resistance and 17 genotypes displayed highly or moderately tolerate to EGA. Genetic diversity of these lines was investigated also. After 97 SSR loci which evenly covered all wheat chromosomes were scanned for association, four SSR loci were significantly associated with EGA resistance and four with EGA tolerance. After association analysis was conducted with dynamic aphid densities, we found four loci Xgwm192b, Xgwm391, Xbarc98, and Xgwm613b were detected continuously at different growing stages of wheat. In addition, the loci of EGA resistance/tolerance and Russian wheat aphid resistance were compared. The results generated in this study would be helpful for utilization of the EGA resistance/tolerance germplasm, and for development of mapping populations in EGA resistance breeding programs.

Abstract English grain aphid (EGA) is a destructive insect pest of wheat. To identify the loci associated with EGA resistance and tolerance, 70 bread wheat accessions mainly from central Asia were evaluated for EGA resistance and tolerance traits at two locations, and genotyped with 51 SSR markers. Totally, three accessions showed high or moderate levels resistance and 17 genotypes displayed highly or moderately tolerate to EGA. Genetic diversity of these lines was investigated also. After 97 SSR loci which evenly covered all wheat chromosomes were scanned for association, four SSR loci were significantly associated with EGA resistance and four with EGA tolerance. After association analysis was conducted with dynamic aphid densities, we found four loci Xgwm192b, Xgwm391, Xbarc98, and Xgwm613b were detected continuously at different growing stages of wheat. In addition, the loci of EGA resistance/tolerance and Russian wheat aphid resistance were compared. The results generated in this study would be helpful for utilization of the EGA resistance/tolerance germplasm, and for development of mapping populations in EGA resistance breeding programs.

Keywords: English grain aphid resistance tolerance association mapping Triticum aestivum

Received: 28 December 2012 Accepted:

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This work was supported by the National Basic Research Program of China (2009CB118300).

Corresponding Authors: PENG Jun-hua, Tel/Fax: +86-27-87510525, E-mail: jpeng@wbgcas, jpeng@lamar.colostate.edu E-mail: jpeng@wbgcas, jpeng@lamar.colostate.edu

About author: LI Feng-qi, E-mail: pandit@163.com

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LI Feng-qi, PENG Jun-hua. 2014. Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm. Journal of Integrative Agriculture, 13(1): 40-53.

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