Identification of QTLsAssociated with Total SoyasaponinContent in Soybean (Glycine max (L.) Merr.)

doi:10.1016/S1671-2927(00)8734

Journal of Integrative Agriculture

2012, Vol. 12

Issue (12): 1976-1984 DOI: 10.1016/S1671-2927(00)8734

Crop Genetics · Breeding · Germplasm Resources

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Identification of QTLsAssociated with Total SoyasaponinContent in Soybean (Glycine max (L.) Merr.)

HUANG Shan-shan, HAN Ying-peng, LI Chang-suo, TIAN Jun, LI Wen-bin, WANG Ji-an

1.Key Laboratory of Soybean Biology, Ministry of Education/Soybean Research Institute, Northeast Agricultural University, Harbin 150030, P.R.China
2.Plant Protection Institute, Heilongjiang Academy of Land Reclamation Sciences, Harbin 150038, P.R.China

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摘要 Soyasaponins are valuable compounds in certain drugs, industry, food additives and surfactants. Selecting cultivars with higher-soyasaponin content along with agronomic traits is a main goal for many soybean breeders. The aim of the present study was to identify the quantitative trait loci (QTLs) associated with total soyasaponin content through a F2 population, which was derived from a cross between Ha 91016 (higher soyasaponin content cultivar, 16.8 mg g-1) and N98-9445A (lower soyasaponin content, only 5.7 mg g-1). A genetic linkage map including a total of 162 simple sequence repeat markers was constructed, which covered the total length 2 735.5 cM, and the average distance between markers was 16.96 cM. Two QTLs associated with total soyasaponin content were identified. One, qSAP_1 (located in sat_044-satt102 of linkage group (LG) K), could explain 12.6% of phenotypic variance. The other, qSAP_2, was located between satt368 and sat_413 of LG D1a, which could explain 15.8% of phenotypic variance. It was concluded that the two QTLs would have some potential value for marker-assisted selection for high-soyasaponin content breeding in soybeans.

Abstract Soyasaponins are valuable compounds in certain drugs, industry, food additives and surfactants. Selecting cultivars with higher-soyasaponin content along with agronomic traits is a main goal for many soybean breeders. The aim of the present study was to identify the quantitative trait loci (QTLs) associated with total soyasaponin content through a F2 population, which was derived from a cross between Ha 91016 (higher soyasaponin content cultivar, 16.8 mg g-1) and N98-9445A (lower soyasaponin content, only 5.7 mg g-1). A genetic linkage map including a total of 162 simple sequence repeat markers was constructed, which covered the total length 2 735.5 cM, and the average distance between markers was 16.96 cM. Two QTLs associated with total soyasaponin content were identified. One, qSAP_1 (located in sat_044-satt102 of linkage group (LG) K), could explain 12.6% of phenotypic variance. The other, qSAP_2, was located between satt368 and sat_413 of LG D1a, which could explain 15.8% of phenotypic variance. It was concluded that the two QTLs would have some potential value for marker-assisted selection for high-soyasaponin content breeding in soybeans.

Keywords: quantitative trait loci (QTL) simple sequence repeats (SSR) marker-assisted selection (MAS) soybean soyasaponin

Received: 06 September 2011 Accepted:

Fund:

This study was supported by the National Natural Science Foundation of China (30471092).

Corresponding Authors: Correspondence WANG Ji-an, Tel: +86-451-55190692, E-mail: wangsoy@yahoo.com.cn

About author: HUANG Shan-shan, E-mail: huangshanshan.6@163.com;

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HUANG Shan-shan, HAN Ying-peng, LI Chang-suo, TIAN Jun, LI Wen-bin, WANG Ji-an. 2012. Identification of QTLsAssociated with Total SoyasaponinContent in Soybean (Glycine max (L.) Merr.). Journal of Integrative Agriculture, 12(12): 1976-1984.

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