|Characterization of chromosome segment substitution lines reveals candidate genes associated with the nodule number in soybean
|ZOU Jia-nan1*, ZHANG Zhan-guo1*, KANG Qing-lin1*, YU Si-yang1, WANG Jie-qi1, CHEN Lin1, LIU Yan-ru1, MA Chao1, ZHU Rong-sheng1, ZHU Yong-xu1, DONG Xiao-hui2, JIANG Hong-wei1, 3, WU Xiao-xia1, WANG Nan-nan1, 4, HU Zhen-bang1, QI Zhao-ming1, LIU Chun-yan1, CHEN Qing-shan1, XIN Da-wei1, WANG Jin-hui1
|1 College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China
2 Suihua Branch of Heilongjiang Academy of Agricultural Sciences, Suihua 152000, P.R.China
3 Soybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, P.R.China
4 Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154000, P.R.China
Abstract Soybean is one of the most important food crops worldwide. Like other legumes, soybean can form symbiotic relationships with Rhizobium species. Nitrogen fixation of soybean via its symbiosis with Rhizobium is pivotal for sustainable agriculture. Type III effectors (T3Es) are essential regulators of the establishment of the symbiosis, and nodule number is a feature of nitrogen-affected nodulation. However, genes encoding T3Es at quantitative trait loci (QTLs) related to nodulation have rarely been identified. Chromosome segment substitution lines (CSSLs) have a common genetic background but only a few loci with heterogeneous genetic information; thus, they are suitable materials for identifying candidate genes at a target locus. In this study, a CSSL population was used to identify the QTLs related to nodule number in soybean. Single nucleotide polymorphism (SNP) markers and candidate genes within the QTLs interval were detected, and it was determined which genes showed differential expression between isolines. Four candidate genes (GmCDPK28, GmNAC1, GmbHLH, and GmERF5) linked to the SNPs were identified as being related to nodule traits and pivotal processes and pathways involved in symbiosis establishment. A candidate gene (GmERF5) encoding a transcription factor that may interact directly with the T3E NopAA was identified. The confirmed CSSLs with important segments and candidate genes identified in this study are valuable resources for further studies on the genetic network and T3Es involved in the signaling pathway that is essential for symbiosis establishment.
Received: 26 November 2020
Accepted: 19 February 2021
|Fund: Financial support was received from the National Natural Science Foundation of China (32070274, 32072014 and 31971899), the China Postdoctoral Science Foundation (2020M681072), the Natural Science Foundation for the Excellent Youth Scholars of Heilongjiang Province, China (YQ2019C008), the Europe Horizon 2020 (EUCLEG and 727312), the Youth Science and Technology Innovation Leader, China (2018RA2172), the National Key Research & Development Program of China (2016YFD0100500, 2016YFD0100300 and 2016YFD0100201), and the Heilongjiang Postdoctoral Science Foundation, China (LBH-Q16014).
|About author: Correspondence CHEN Qing-shan, Tel: +86-451-55191945, Fax: +86-451-55191863, E-mail: email@example.com; XIN Da-wei, Tel: +86-451-55191945, Fax: +86-451-55190153, E-mail: firstname.lastname@example.org; WANG Jin-hui, Tel: +86-451-55191945, Fax: +86-451-55190153, E-mail: email@example.com
* These authors contributed equally to this study.
Cite this article:
ZOU Jia-nan, ZHANG Zhan-guo, KANG Qing-lin, YU Si-yang, WANG Jie-qi, CHEN Lin, LIU Yan-ru, MA Chao, ZHU Rong-sheng, ZHU Yong-xu, DONG Xiao-hui, JIANG Hong-wei, WU Xiao-xia, WANG Nan-nan, HU Zhen-bang, QI Zhao-ming, LIU Chun-yan, CHEN Qing-shan, XIN Da-wei, WANG Jin-hui.
Characterization of chromosome segment substitution lines reveals candidate genes associated with the nodule number in soybean. Journal of Integrative Agriculture, 21(8): 2197-2210.
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