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| Omethoate-Induced Changes of (+)-d-Cadinene Synthase Activity and Gossypol Content in Cotton Seedlings |
| YANG Wen-ling, SHI Xue-yan, ZHONG Li, LIANG Pei, SONG Dun-lun, GAO Xi-wu |
| Department of Entomology, China Agricultural University, Beijing 100193, P.R.China |
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摘要 The gene expression and activity of (+)-d-cadinene synthase during cotton development and in response to stress, as well as the spatial and temporal pattern of sesquiterpene biosynthesis, constitute one of chemical defense mechanisms in cotton plants. In order to explore the effects of omethoate on the cotton defense in relation to (+)-d-cadinene synthase and gossypol, effects of omethoate treatments on activity of (+)-d-cadinene synthase and gossypol content in cotton seedlings were investigated. Cotton seedlings treated with 400 mg L-1 omethoate exhibited a significant decrease in the specific activity of (+)-d-cadinene synthase from 12 to 120 h after treating when compared to the untreated control; significantly lower (+)-d-cadinene synthase activity was also observed in cotton seedlings treated with 200 mg L-1 omethoate from 72 to 120 h after treating; but for cotton seedlings treated with 100 mg L-1 omethoate, from 12 to 120 h after treating, no significant changes were observed for activity of (+)-d-cadinene synthase. The gossypol content in cotton seedlings treated with 100, 200 or 400 mg L-1 omethoate for different time periods showed no significant changes compared to that of the control. These results indicated that the activity of (+)-d-cadinene synthase in cotton seedlings in responses to exposure of omethoate at three concentrations for different time periods followed dose- and time-dependent responses to omethoate exposure. With omethoate as a chemical stress factor for cotton seedlings, the cotton defense in relation to the activity of (+)-d-cadinene synthase in cotton seedlings may be affected by omethoate application.
Abstract The gene expression and activity of (+)-d-cadinene synthase during cotton development and in response to stress, as well as the spatial and temporal pattern of sesquiterpene biosynthesis, constitute one of chemical defense mechanisms in cotton plants. In order to explore the effects of omethoate on the cotton defense in relation to (+)-d-cadinene synthase and gossypol, effects of omethoate treatments on activity of (+)-d-cadinene synthase and gossypol content in cotton seedlings were investigated. Cotton seedlings treated with 400 mg L-1 omethoate exhibited a significant decrease in the specific activity of (+)-d-cadinene synthase from 12 to 120 h after treating when compared to the untreated control; significantly lower (+)-d-cadinene synthase activity was also observed in cotton seedlings treated with 200 mg L-1 omethoate from 72 to 120 h after treating; but for cotton seedlings treated with 100 mg L-1 omethoate, from 12 to 120 h after treating, no significant changes were observed for activity of (+)-d-cadinene synthase. The gossypol content in cotton seedlings treated with 100, 200 or 400 mg L-1 omethoate for different time periods showed no significant changes compared to that of the control. These results indicated that the activity of (+)-d-cadinene synthase in cotton seedlings in responses to exposure of omethoate at three concentrations for different time periods followed dose- and time-dependent responses to omethoate exposure. With omethoate as a chemical stress factor for cotton seedlings, the cotton defense in relation to the activity of (+)-d-cadinene synthase in cotton seedlings may be affected by omethoate application.
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Received: 05 May 2011
Accepted:
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| Fund: Acknowledgements This work was supported by the National Natural Science Foundation of China (30771426), the National “973” Program of China (2006CB102003), the Mechanisms and Detection of Insecticide Resistance of Important Pests Insects in China and Japan (NSFC and JSPS, 30911140107), and the Program for China New Century Excellent Talents in University of China (NCET-06-0113). |
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Corresponding Authors:
Correspondence SHI Xue-yan, Tel/Fax: +86-10-62731306, E-mail: shixueyan@cau.edu.cn; GAO Xi-wu, Tel/Fax: +86-10-62732974, E-mail: xiwugao@263.net.cn
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Cite this article:
YANG Wen-ling, SHI Xue-yan, ZHONG Li, LIANG Pei, SONG Dun-lun, GAO Xi-wu.
2012.
Omethoate-Induced Changes of (+)-d-Cadinene Synthase Activity and Gossypol Content in Cotton Seedlings. Journal of Integrative Agriculture, 12(10): 1682-1690.
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| [1]Alborn T, Turlings T C J, Jones T H, Stenhagen G, LoughrinJ H, Tumlinson J H. 1997. An elicitor of plant volatilesfrom beet armyworm oral secretion. Science, 276, 945-949.[2]Alchanati I, Patel J A, Liu J, Benedict C R, Stipanovic R D,Bell A A, Cui Y X, Magill C W. 1998. The enzymiccyclization of nerolidyl diphosphate by d-cadinenesynthase from cotton stele tissue infected withVerticillium dahliae. Phytochemistry, 47, 961-967.[3]Arimura G, Kost C, Boland W. 2005. Herbivore-induced,indirect plant defences. Biochimica et Biophysica Acta,1734, 91-111.[4]Arimura G, Ozawa R, Shimoda T, Nishioka T, Boland W,Takabayashi J. 2000. Herbivory-induced volatiles elicitdefence genes in lima bean leaves. Nature, 406, 512-514.[5]Benedict C R, Alchanati I, Harvey P J, Liu J G, Stipanovic RD, BellAA. 1995. The enzymatic formation of d-cadinenefrom farnesyl diphosphate in extracts of cotton.Phytochemistry, 39, 327-331.[6]Benedict C R, Lu J L, Pettigrew D W, Liu J G, Stipanovic RD, Williams J H. 2001. The cyclization of farnesyldiphosphate and nerolidyl diphosphate by a purifiedrecombinant d-cadinene synthase. Plant Physiology,125, 1754-1765.[7]Bianchini G M, Stipanovic R D, Bell AA. 1999. Induction ofd-cadinene synthase and sesquiterpenoid phytoalexinsin cotton by Verticillium dahliae. Journal ofAgricultural and Food Chemistry, 47, 4403-4406.[8]Bradford M M. 1976. A rapid and sensitive method for thequantification of microgram quantities of protein usingthe principle of protein-dye binding. AnalyticalBiochemistry, 72, 248-354.[9]Chen M S. 2008. Inducible direct plant defense againstinsect herbivores: a review. Insect Science, 15, 101-114.[10]Chen X Y, Chen Y, Heinstein P, Davisson VJ. 1995. Cloning,expression, and characterization of (+)-d-cadinenesynthase: a catalyst for cot ton phytoalexinbiosynthesis. Archives of Biochemistry and Biophysics,324, 255-266.[11]Chen X Y, Wang M S, Chen Y, Davisso V J, Heinstein P.1996. Cloning and heterologous expression of a second(+)-d-cadinene synthase from Gossypium arboretum.Journal of Natural Products, 59, 944-951.[12]Dalzell S A, Mullen B F. 2004. Application of pesticidessuppress foliar proanthocyanidin content in Leucaenaspecies. Animal Feed Science and Technology, 113, 191-198.[13]Davis G D, Essenberg M. 1995. (+)-d-Cadinene is a productof sesqui t e rpene cyclase activi ty in cot ton.Phytochemistry, 39, 553-567.[14]Davis D, Merida J, Legendre L, Low P S, Heinstein P. 1992.Independent elicitation of the oxidative burst andphytoalexin formation in cultured plant cells.Phytochemistry, 32, 607-610.[15]Dicke M, Baarlen P, Wessels R, Dokman H. 1993. Herbivoryinduces systemic production of plant volatiles thatattract herbivore predators: extraction of endogenouselicitor. Journal of Chemical Ecology, 19, 581-599.[16]Farmer E E, Ryan C A. 1990. Interplant communication:Airborne methyl jasmonate induces synthesis ofproteinase inhibitors in plant leaves. Proceedings ofthe National Academy of Sciences of the United Statesof America, 87, 7713-7716.[17]Ford K A, Casida J E, Chandran D, Gulevich A G, Okrent RA, Durkin K A, Sarpong R, Bunnelle E M, WildermuthM C. 2010. Neonicotinoid insecticides induce salicylateassociatedplant defense responses. Proceedings ofthe National Academy of Sciences of the United Statesof America, 107, 17527-17532.[18]Halitschke R, Schittko U, Pohnert G, Boland W, Baldwin IT. 2001. Molecular interactions between the specialistherbivore Manduca sexta (Lepidoptera, Sphingidae)and its natural host Nicotiana attenuate. II. Fatty acidaminoacid conjugates in herbivore oral secretions arenecessary and sufficient for herbivore-specific plantresponses. Plant Physiology, 125, 711- 717.Hedin P A, Parrott N L, Jenkins J N. 1992. Relationship ofglands, cotton square terpenoid aldehydes and otherallelochemicals to larval growth of Heliothis virescens(Lepidoptera: Noctuidae). Journal of EconomicEntomology, 85, 359-364.[19]Heinstein P. 1985. Stimulation of sesquiterpene aldehydeformation in Gossypium arboreum cell suspensioncultures by conidia of Verticillium dahliae. Journal ofNatural Products, 48, 907-915.[20]Hoagland D R, Arnon D I. 1950. The water culture methodfor growing plants without soil. In: CaliforniaAgricultural Experiment Station Circular 347.University of California at Berkeley, USA. pp. 1-32.[21]Howell C R, Hanson L E, Stipanovic R D, Puckhaber L S.2000. Induction of terpenoid synthesis in cotton rootsand control of Rhizoctonia solani by seed treatmentwith Trichoderma virens. Phytopathology, 90, 248-252.[22]Jenkins J N, Hedin P A, Parrott W L, McCartyjr J C, WhiteWH. 1983. Cotton allelochemicals and growth of tobaccobudworm larvae. Crop Science, 23, 1195-1198.[23]Karban R. 1984. Induced resistance of cotton seedlings tomites. Science, 225, 53-54.[24]Kranthi S, Kranthi K R, Wanjari R R. 2003. Influence ofsemilooper damage on cotton host-plant resistance toHelicoverpa armigera (H黚.). Plant Science, 164, 157-163.[25]Lane H C, Schuster M F. 1981. Condensed tannins of cottonleaves. Phytochemistry, 20, 425-427.[26]Litvak M E, Monson R K. 1998. Patterns of induced andconstitutive monoterpene production in conifer needlesin relation to insect herbivory. Oecologia, 114, 531-540.[27]Liu C J, Heinstein P, Chen X Y. 1999. Expression pattern ofgenes encoding farnesyl diphosphate synthase andsesquiterpene cyclase in cotton suspension-culturedcells treated with fungal elicitors. Molecular Plant-Microbe Interactions, 12, 1095-1104.[28]Mao Y B, Lu S, Wang L J, Chen X Y. 2006. Biosynthesis ofgossypol in cotton. CAB Reviews: Perspective inAgriculture, Veterinary Science, Nutrition andNatural Resources, 49, 1-12.[29]Martin G S, Liu J G, Benedict C R, Stipanovic R D, Magill CW. 2003. Reduced levels of cadinane sesquiterpenoidsin cotton plants expressing antisense (+)-d-cadinenesynthase. Phytochemistry, 62, 31-38.[30]Mattiacci L, Dicke M, Posthumus M A. 1995. Betaglucosidase:an elicitor of herbivore-induced plant orderthat at t racts host-searching parasi tic wasps .Proceedings of the National Academy of Sciences ofthe United States of America, 92, 2036-2040.[31]McFadden H G, Chapple R, DeFeyter R, Dennis E. 2001.Expression of pathogenesis-related genes in cottonstems in response to infection by Verticillium dahliae.Physiological and Molecular Plant Pathology, 58,119-131.[32]Meyer R, Vorster S, Dubery I A. 2004. Identification andquantification of gossypol in cotton by using packedmicro-tips columns in combination with HPLC.Analytical and Bioanalytical Chemistry, 380, 719-724.[33]Nomeir A A, Abou-Donia M B. 1982. Gossypol: highperformanceliquid chromatographic analysis andstability in various solvents. Journal of the AmericanOil Chemists?Society, 59, 546-549.[34]Saladin G, Cl閙ent C. 2005. Physiological side effects ofpesticides on non-target plants. In: Agriculture andSoil Pollution. Nova Science Publishers, Inc. New York,pp. 53-86.[35]Santhoshkumar K, Prakashkumar B, Mathew M M. 2009.Effect of carbofuran on defence related enzymes in thecalli of Cucumis sativus Linn. Plant Cell Biotechnologyand Molecular Biology, 10, 101-106.[36]Schnee C, K鰈lner T G, Held M, Turlings T C J, GershenzonJ, Degenhardt J. 2006. The products of a single maizesesquiterpene synthase form a volatile defense signalthat attracts natural enemies of maize herbivores.Proceedings of the National Academy of Sciences ofthe United States of America, 103, 1129-1134.[37]Shulaev V, Silverman P, Raskin I. 1997. Airborne signalingby methyl salicylate in plant pathogen resistance.Nature, 385, 718-721.[38]Stipanovic R D, Altman D W, Begin D L, Greenblatt G A,Benedict J H. 1988. Terpenoid aldehydes in upland cottons: analysis by aniline and HPLC methods. Journalof Agricultural and Food Chemistry, 36, 509-515.[39]Tang D L, Wang W G, Tan W J, Guo Y Y. 1997. Changes ofcontents of some substances in cotton leaves inducedby cotton bollworm Helicoverpa armigera (Huebner)attack. Acta Entomologica Sinica, 40, 332-333.[40](inChinese)Tomlin E S, Antonejevic E, Alfaro R I, Borden J H. 2000.Changes in volatile terpene and diterpene resin acidcomposition of resistant and susceptible white spruceleaders exposed to simulated white pine weevil damage.Tree Physiology, 20, 1087-1095.[41]Townsend B J, Poole A, Blake C J, Llewellyn D J. 2005.Antisense suppression of a (+)-d-cadinene synthasegene in cotton prevents the induction of this defenseresponse gene during bacterial blight infection but notits constitutive expression. Plant Physiology, 138, 516-528.[42]Xu Y H, Wang J W, Wang S, Wang J Y, Chen X Y. 2004.Characterization of GaWRKY1, a cotton transcriptionfactor that regulates the sesquiterpene synthase gene(+)-d-cadinene synthase-A. Plant Physiology, 135, 507-515.[43]Zhang YJ,WangWG, Guo YY. 2001. Analysis of terpenoidsand their spatio-temporal expression of content in Bttransgenic cotton by HPLC method. Chinese Journalof Applied and Environmental Biology, 7, 37-40. 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