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Journal of Integrative Agriculture  2023, Vol. 22 Issue (8): 2306-2322    DOI: 10.1016/j.jia.2023.01.002
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Comparative transcriptome and lipidome reveal that a low K+ signal effectively alleviates the effect induced by Ca2+ deficiency in cotton fibers
GUO Kai1*, GAO Wei2*, ZHANG Tao-rui1, WANG Zu-ying1, SUN Xiao-ting1, YANG Peng1, LONG Lu2, LIU Xue-ying1, WANG Wen-wen1, TENG Zhong-hua1, LIU Da-jun1, LIU De-xin1, TU Li-li3, ZHANG Zheng-sheng1#
1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, P.R.China
2 State Key Laboratory of Cotton Biology/Henan Key Laboratory of Plant Stress Biology/School of Life Sciences, Henan University,
Kaifeng 475001, P.R.China
3 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China
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Abstract  Calcium (Ca2+) plays an important role in determining plant growth and development because it maintains cell wall and
membrane integrity. Therefore, understanding the role of Ca2+ in carbon and lipid metabolism could provide insights
into the dynamic changes in cell membranes and cell walls during the rapid elongation of cotton fibers. In the present
study, we found that the lack of Ca2+ promoted fiber elongation and rapid ovule expansion, but it also caused tissue
browning in the ovule culture system. RNA-sequencing revealed that Ca2+ deficiency induced cells to be highly oxidized,
and the expression of genes related to carbon metabolism and lipid metabolism was activated significantly. All gene
members of nine key enzymes involved in glycolysis were up-regulated, and glucose was significantly reduced in Ca2+
deficiency-treated tissues. Ca2+ deficiency adjusted the flowing of glycolysis metabolic. However, low K+ recovered
the expression levels of glycolysis genes and glucose content caused by Ca2+ deficiency. Electrospray ionizationtandem
mass spectrometry technology was applied to uncover the dynamic profile of lipidome under Ca2+ and K+
interacted conditions. Ca2+ deficiency led to the decrease of fatty acid (FA), diacylglycerol (DAG), glycolipid and the
significant increase of triacylglycerol (TAG), phospholipid phosphatidylethanolamine (PE), phosphatidylglycerol (PG),
and PC (phosphatidylcholine). Low K+ restored the contents of FA, phospholipids, and glycolipids, effectively relieved
the symptoms caused by Ca2+ deficiency, and recovered the development of fiber cells. This study revealed dynamic
changes in transcript and metabolic levels and uncovered the signaling interaction of Ca2+ deficiency and low K+ in
glycolysis and lipid metabolism during fiber development.
Keywords:  cotton fiber        glycolysis       lipidome        calcium        potassium  
Received: 10 August 2022   Accepted: 12 October 2022

This project was supported by the National Natural Science Foundation of China (31901577), the Fundamental Research Funds for the Central Universities, China (SWU-KT22035), and the State Key Laboratory of Cotton Biology Open Fund, China (CB2021A32).

About author:  #Correspondence ZHANG Zheng-sheng, E-mail: * These authors contributed equally to this study.

Cite this article: 

GUO Kai, GAO Wei, ZHANG Tao-rui, WANG Zu-ying, SUN Xiao-ting, YANG Peng, LONG Lu, LIU Xue-ying, WANG Wen-wen, TENG Zhong-hua, LIU Da-jun, LIU De-xin, TU Li-li, ZHANG Zheng-sheng. 2023. Comparative transcriptome and lipidome reveal that a low K+ signal effectively alleviates the effect induced by Ca2+ deficiency in cotton fibers. Journal of Integrative Agriculture, 22(8): 2306-2322.

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