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Journal of Integrative Agriculture
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Comparative transcriptome and lipidome reveal that low K+ signal effectively alleviate the effect induced by Ca2+ deficiency in fiber of cotton (Gossypium hirsutum)
GUO Kai1*, GAO Wei2*, ZHANG Tao-rui1, WANG Zu-ying1, SUN Xiao-ting1, YANG Peng1, LONG Lu2, LIU Xue-ying1, WANG Wen-wen1, TENG Zhong-hua1, LIU Da-jun1, LIU De-xin1, TU Li-li3, ZHANG Zheng-sheng1

1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, P.R.China

2 State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, P.R.China

3 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China

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Calcium (Ca2+) plays important roles in determining plant growth and development owing to its function in maintaining cell wall and membrane integrity.  Therefore, understanding the role of Ca2+ in carbon and lipid metabolism could provide insights into the dynamic changes of cell membranes and cell walls during rapid elongation of cotton fibers.  In the present study, we found that the lack of Ca2+ promoted fiber elongation and ovule rapid expansion, but also caused tissue browning in ovule culture system.  RNA-sequencing revealed that Ca2+ deficiency induced cells to be in a highly oxidized state, and the expression of genes related to carbon metabolism and lipid metabolism was activated significantly.  All gene members of 9 key enzymes involving in glycolysis were up-regulated, and glucose was significantly reduced in Ca2+ deficiency treated tissues.  Ca2+ deficiency adjusted the flowing of glycolysis metabolic.  However, low K+ recovered the expression levels of glycolysis genes and glucose content caused by Ca2+ deficiency.  Electrospray ionization-tandem mass spectrometry technology was applied to uncover the dynamic profile of lipidome under Ca2+ and K+ interacted condition.  Ca2+ deficiency led to the decrease of fatty acid (FA), diacylglycerol (DAG) and glycolipid, and the significant increase of triacylglycerol (TAG), phospholipid PE (phosphatidylethanolamine), PG (phosphatidylglycerol) and PC (phosphatidylcholine).  Low K+ restored the contents of FA, phospholipids and glycolipids, effectively relieved the symptoms caused by Ca2+ deficiency, and recovered the development of fiber cells.  This study revealed dynamic changes in transcript and metabolic levels, and uncovered the signaling interaction of Ca2+ deficiency and low K+ in glycolysis and lipid metabolism during fiber development.

Keywords:  cotton fiber        glycolysis       lipidome        calcium        potassium  
Online: 10 January 2023  

This project was supported by the National Natural Science Foundation of China (31901577), the Fundamental Research Funds for the Central Universities (SWU-KT22035) and the State Key Laboratory of Cotton Biology Open Fund (CB2021A32).

About author:  Correspondence ZHANG Zheng-sheng, E-mail: * These authors have contributed equally to this work.

Cite this article: 

GUO Kai, GAO Wei, ZHANG Tao-rui, WANG Zu-ying, SUN Xiao-ting, YANG Peng, LONG Lu, LIU Xue-ying, WANG Wen-wen, TENG Zhong-hua, LIU Da-jun, LIU De-xin, TU Li-li, ZHANG Zheng-sheng. 2023. Comparative transcriptome and lipidome reveal that low K+ signal effectively alleviate the effect induced by Ca2+ deficiency in fiber of cotton (Gossypium hirsutum). Journal of Integrative Agriculture, Doi:10.1016/j.jia.2023.01.002

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