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Journal of Integrative Agriculture  2023, Vol. 22 Issue (7): 2000-2014    DOI: 10.1016/j.jia.2022.10.007
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A 2-bp frameshift deletion at GhDR, which encodes a B-BOX protein that co-segregates with the dwarf-red phenotype in Gossypium hirsutum L.

WANG Xue-feng1*, SHAO Dong-nan1*, LIANG Qian1, FENG Xiao-kang1, ZHU Qian-hao2, YANG Yong-lin3, LIU Feng1, ZHANG Xin-yu1, LI Yan-jun1, SUN Jie1#, XUE Fei1#

1 Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi 832000, P.R.China 

2 CSIRO Agriculture and Food, Canberra, ACT 2601, Australia

3 Cotton Research Institute, Shihezi Academy of Agriculture Sciences, Shihezi 832000, P.R.China

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株型和叶色是棉花纤维产量的重要影响因素。本研究基于遗传分析、茎秆石蜡切片和植物激素处理方法,发现棉花矮红突变体DR一个赤霉素敏感型突变体,由一个单显性基因位点突变引起,将其命名为GhDR。通过BSA-seq结合靶向测序基因型检测GBTS方法控制突变性状基因定位A09 染色体约197 kb候选区间包含 25 个注释基因。基于候选基因的注释信息及其在突变体和正常植株之间的序列和表达差异GH_A09G2280基因被认为是控制矮红突变体表型的最佳候选基因。在DR突变体GhDR/GH_A09G2280基因编码区发现一个2 bp缺失,导致GhDR基因产生移码突变,蛋白翻译提前终止GhDR是拟南芥AtBBX24的同源基因,编码B-box锌指蛋白。移码缺失导致GhDR C末端缺失核定位结构域和VP结构,并改变了其亚细胞定位结果比较转录组分析表明,在DR突变体中,参与赤霉素生物合成和信号转导的关键基因下调表达,而与赤霉素降解和花青素生物合成相关基因上调表达。研究初步揭示了GhDR基因同时调控棉花株型和花青素积累的潜在分子机制

Abstract  Plant architecture and leaf color are important factors influencing cotton fiber yield. In this study, based on genetic analysis, stem paraffin sectioning, and phytohormone treatments, we showed that the dwarf-red (DR) cotton mutant is a gibberellin-sensitive mutant caused by a mutation in a single dominant locus, designated GhDR. Using bulked segregant analysis (BSA) and genotyping by target sequencing (GBTS) approaches, we located the causative mutation to a ~197-kb genetic interval on chromosome A09 containing 25 annotated genes. Based on gene annotation and expression changes between the mutant and normal plants, GH_A09G2280 was considered to be the best candidate gene responsible for the dwarf and red mutant phenotypes. A 2-nucleotide deletion was found in the coding region of GhDR/GH_A09G2280 in the DR mutant, which caused a frameshift and truncation of GhDR. GhDR is a homolog of Arabidopsis AtBBX24, and encodes a B-box zinc finger protein. The frameshift deletion eliminated the C-terminal nuclear localization domain and the VP domain of GhDR, and altered its subcellular localization. A comparative transcriptome analysis demonstrated downregulation of the key genes involved in gibberellin biosynthesis and the signaling transduction network, as well as upregulation of the genes related to gibberellin degradation and the anthocyanin biosynthetic pathway in the DR mutant. The results of this study revealed the potential molecular basis by which plant architecture and anthocyanin accumulation are regulated in cotton.  

Keywords:  cotton       BBX        dwarf        anthocyanin        gibberellin  
Received: 04 July 2022   Accepted: 25 August 2022
Fund: This work was supported by the National Natural Science Foundation of China (32160477 and 31960412), the International S&T Cooperation Projects of BINGTUAN, China (2021BC001), and the Young and Middle-aged Leaders in Scientific and Technological Innovation Foundation of Shihezi, China (2021RC02 and 2020CB010).
About author:  #Correspondence XUE Fei, E-mail:; SUN Jie, E-mail: * These authors contributed equally to this study

Cite this article: 

WANG Xue-feng, SHAO Dong-nan, LIANG Qian, FENG Xiao-kang, ZHU Qian-hao, YANG Yong-lin, LIU Feng, ZHANG Xin-yu, LI Yan-jun, SUN Jie, XUE Fei. 2023. A 2-bp frameshift deletion at GhDR, which encodes a B-BOX protein that co-segregates with the dwarf-red phenotype in Gossypium hirsutum L.. Journal of Integrative Agriculture, 22(7): 2000-2014.

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