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| Fine Mapping of qTGW3-1, a QTL for 1000-Grain Weight on Chromosome 3 in Rice |
| ZHANG Qiang, YAO Guo-xin, HU Guang-long, TANG Bo, ZHANG Hong-liang, LI Zi-chao |
1.Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
2.Rice Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, P.R.China
3.School of Life Science and Technology, Xiaogan University, Xiaogan 432100, P.R.China |
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摘要 The QTL qTGW3-1 was located on chromosome 3 of rice (Oryza sativa L.) and associated with the 1 000-grain weight (TGW) according to the result of our earlier study. With the objective of fine mapping of this locus, we developed a F2 population consisting of 3 428 plants derived from the cross between TGW-related near isogenic line DL017 (BC3F4 generation of GSL156×Nipponbare) and the recurrent parent Nipponbare. Using six microsatellites, this QTL was delimited between RM5477 and RM6417. Markers MM1455 and MM1456 within this region were used for further mapping of this QTL. Finally, qTGW3-1 was fine-mapped into a 89-kb interval between RM5477 and MM1456, which locates in the BAC clone AC107226 harboring five putative candidate genes.
Abstract The QTL qTGW3-1 was located on chromosome 3 of rice (Oryza sativa L.) and associated with the 1 000-grain weight (TGW) according to the result of our earlier study. With the objective of fine mapping of this locus, we developed a F2 population consisting of 3 428 plants derived from the cross between TGW-related near isogenic line DL017 (BC3F4 generation of GSL156×Nipponbare) and the recurrent parent Nipponbare. Using six microsatellites, this QTL was delimited between RM5477 and RM6417. Markers MM1455 and MM1456 within this region were used for further mapping of this QTL. Finally, qTGW3-1 was fine-mapped into a 89-kb interval between RM5477 and MM1456, which locates in the BAC clone AC107226 harboring five putative candidate genes.
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Received: 25 February 2011
Accepted:
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| Fund: This work was supported by the National Basic Research Program of China (2010CB129504), the National Key Technologies R&D Program of China (2009BADA2B01), the 948 Project of MOA, China (2011-G2B). We thank Ms. Li Ding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, for critically reading and revising this manuscript. |
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Corresponding Authors:
LI Zi-chao, Tel: +86-10-62731414, E-mail: lizichao@cau.edu.cn
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| About author: ZHANG Qiang, Tel: +86-434-6351744, E-mail: zhqiang73@yahoo.com.cn; |
Cite this article:
ZHANG Qiang, YAO Guo-xin, HU Guang-long, TANG Bo, ZHANG Hong-liang, LI Zi-chao .
2012.
Fine Mapping of qTGW3-1, a QTL for 1000-Grain Weight on Chromosome 3 in Rice. Journal of Integrative Agriculture, 12(6): 879-887.
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