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Special Issue:
园艺-分子生物合辑Horticulture — Genetics · Breeding
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| Integration of the metabolome and transcriptome reveals the metabolites and genes related to nutritional and medicinal value in Coriandrum sativum |
WU Tong1, FENG Shu-yan1, YANG Qi-hang1, Preetida J BHETARIYA2, GONG Ke1, CUI Chun-lin1, SONG Jie1, PING Xiao-rui1, PEI Qiao-ying1, YU Tong1, SONG Xiao-ming1, 3, 4 |
1 College of Life Sciences, North China University of Science and Technology, Tangshan 063210, P.R.China
2 Department of Biostatistics, Harvard School of Public Health, Boston, MA 02115, USA
3 Food Science and Technology Department, University of Nebraska-Lincoln, Lincoln, NE 68526, USA
4 Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, P.R.China |
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摘要
芫荽或胡荽是一种具有多种药效的药膳，广泛用于烹饪和传统医药。它富含精油和抗氧化剂化合物，但其意义不明。为了发掘这些尚未开发的营养或药用丰富的化合物，我们对芫荽三个发育阶段的转录组学和代谢组学数据进行研究。在此，我们鉴定了10个酪氨酸代谢途径相关基因，6个卟啉和叶绿素代谢途径相关基因，5个维生素E代谢途径相关基因。这些基因与芫荽早期发育相关。我们的分析表明，这些途径参与关键酚类代谢物的产生。此外，我们还构建了这些途径相关基因与转录因子之间的相互作用网络，支持了酚类代谢物的调控通路。有趣的是，我们鉴定到了一些营养或药用成分代谢物，包括59种酚类，2种多胺，12种生物碱和1种萜类。较高含量的代谢物来自于咖啡酸、胍丁胺及其衍生物。我们发现咖啡酸和胍丁胺在30天时的浓度高于60天或90天时的浓度。本研究为进一步研究这些化合物在医学和营养学研究中的作用提供了线索。
Abstract Coriandrum sativum (Coriander) or Chinese parsley is a culinary herb with multiple medicinal effects, which is widely used in cooking and traditional medicine. It is enriched with essential oils and anti-oxidant compounds with unknown significance. To explore the untapped reservoir of Coriander, we studied the transcriptome and metabolic profiles from three developmental stages. Here, we identified 10 tyrosine metabolic pathway-related genes (TMPRGs), six porphyrins and chlorophyll metabolic pathway-related genes (PCMPRGs), and five Vitamin E metabolic pathway-related genes (VEMPRGs). These genes were associated with the early development of Coriander. Our analysis suggests that these pathways are involved in the production of critical phenolic metabolites. Furthermore, we constructed the interaction network between these pathway-related genes and transcription factors (TFs), which supported the regulatory pathways for phenolic metabolites. Interestingly, we identified several nutritional or medicinally relevant metabolites, including 59 phenols, two polyamines, 12 alkaloids, and one terpenoid. The higher concentrations of metabolites were from caffeic acid, agmatine, and its derivatives. We found higher levels of caffeic acid and agmatine at 30 days compared to 60 or 90 days. This study provides evidence to stimulate further investigation of the role of these metabolites in medicinal and nutritional research.
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Received: 24 March 2020
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31801856), the Hebei Province Higher Education Youth Talents Program, China (BJ2018016), and the Hebei Province Postgraduate Demonstration Course (Genomics) Construction Project in 2018, China (KCJSX2018053). |
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Corresponding Authors:
Correspondence SONG Xiao-ming, Tel/Fax: +86-315-8805607, E-mail: songxm@ncst.edu.cn
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| About author: WU Tong, E-mail: WuTongHBLG@163.com |
Cite this article:
WU Tong, FENG Shu-yan, YANG Qi-hang, Preetida J BHETARIYA, GONG Ke, CUI Chun-lin, SONG Jie, PING Xiao-rui, PEI Qiao-ying, YU Tong, SONG Xiao-ming.
2021.
Integration of the metabolome and transcriptome reveals the metabolites and genes related to nutritional and medicinal value in Coriandrum sativum. Journal of Integrative Agriculture, 20(7): 1807-1818.
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