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| Molecular cloning and functional characterization of MdPIN1 in apple |
| AN Jian-ping1*, LIU Xin 1*, LI Hao-hao1, YOU Chun-xiang1, SHU Jing2, WANG Xiao-fei1, HAO Yu-jin1 |
1 National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology/College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
2 Shandong Agriculture and Engineering University, Jinan 251100, P.R.China |
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Abstract Auxin has been identified to play critical roles in regulating plant growth and development. The polar transport of auxin is regulated by auxin transporters. In the present study, an auxin efflux carrier gene MdPIN1 was cloned from Malus×domestic, Royal Gala, and introduced into wild-type Arabidopsis thaliana (Col-0). The transgenic plants exhibited the phenotype of inhibition of primary root (PR) elongation and increased lateral root (LR) number in compared with Col-0. Overexpression of MdPIN1 affected auxin transport, and enhanced phototropic responses and geotropism reaction, whereas had no significant difference in the auxin biosynthesis. These findings suggest that the MdPIN1 gene plays a vital role in auxin transport and root development.
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Received: 19 June 2016
Accepted:
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| Fund: This work was supported by grants from the National Natural Science Foundation of China (31601742), and the Natural Science Foundation of Shandong Province, China (ZR2011CQ007). |
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Corresponding Authors:
HAO Yu-jin, E-mail: haoyujin@sdau.edu.cn; WANG Xiao-fei, E-mail: xfwang2004@163.com
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| About author: AN Jian-ping, Mobile: +86-18854886284; E-mail: 1393427413@qq.com |
Cite this article:
AN Jian-ping, LIU Xin, LI Hao-hao, YOU Chun-xiang, SHU Jing, WANG Xiao-fei, HAO Yu-jin.
2017.
Molecular cloning and functional characterization of MdPIN1 in apple. Journal of Integrative Agriculture, 16(05): 1103-1111.
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