|Functional analysis of MdSUT2.1, a plasma membrane sucrose transporter from apple
ZHANG Bo1, 2, FAN Wen-min1, ZHU Zhen-zhen1, 2, WANG Ying1, ZHAO Zheng-yang1, 2#
1 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
2 Shaanxi Research Center of Apple Engineering and Technology, Yangling 712100, P.R.China
Sugar content is a determinant of apple (Malus×domestica Borkh.) sweetness. However, the molecular mechanism underlying sucrose accumulation in apple fruit remains elusive. Herein, this study reported the role of the sucrose transporter MdSUT2.1 in the regulation of sucrose accumulation in apples. The MdSUT2.1 gene encoded a protein with 612 amino acid residues that could be localized at the plasma membrane when expressed in tobacco leaf protoplasts. MdSUT2.1 was highly expressed in fruit and was positively correlated with sucrose accumulation during apple fruit development. Moreover, complementary growth assays in a yeast mutant validated the sucrose transport activity of MdSUT2.1. MdSUT2.1 overexpression in apples and tomatoes resulted in significant increases in sucrose, fructose, and glucose contents compared to the wild type (WT). Further analysis revealed that the expression levels of sugar metabolism- and transport-related genes SUSYs, NINVs, FRKs, HXKs, and TSTs increased in apples and tomatoes with MdSUT2.1 overexpression compared to WT. Finally, unlike the tonoplast sugar transporters MdTST1 and MdTST2, the promoter of MdSUT2.1 was not induced by exogenous sugars. These findings provide valuable insights into the molecular mechanism underlying sugar accumulation in apples.
Received: 09 May 2022
Accepted: 26 July 2022
|Fund: This work was supported by the earmarked fund for China Agriculture Research System (CARS-27).
|About author: ZHANG Bo, E-mail: email@example.com; #Correspondence ZHAO Zheng-yang, Tel: +86-29-87082922, E-mail: firstname.lastname@example.org
Cite this article:
ZHANG Bo, FAN Wen-min, ZHU Zhen-zhen, WANG Ying, ZHAO Zheng-yang.
Functional analysis of MdSUT2.1, a plasma membrane sucrose transporter from apple. Journal of Integrative Agriculture, 22(3): 762-775.
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