园艺-分子生物合辑Horticulture — Genetics · Breeding
|Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple
|HU Ling-yu, YUE Hong, ZHANG Jing-yun, LI Yang-tian-su, GONG Xiao-qing, ZHOU Kun, MA Feng-wang
|State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
Abstract Myo-inositol and its derivatives play important roles in the tolerance of higher plants to abiotic stresses, and myo-inositol-1-phosphate synthase (MIPS) is the rate-limiting enzyme in myo-inositol biosynthesis. In this study, we found that increased myo-inositol biosynthesis enhanced drought tolerance in MdMIPS1-overexpressing apple lines under short-term progressive drought stress. The effect of myo-inositol appeared to be mediated by the increased accumulation of osmoprotectants such as glucose, sucrose, and proline, and by the increased activities of antioxidant enzymes that eliminate reactive oxygen species. Moreover, enhanced water-use efficiency (WUE) was observed in MdMIPS1-overexpressing apple lines under long-term moderate water deficit conditions that mimicked the water availability in the soil of the Loess Plateau. Enhanced WUE may have been associated with the synergistic regulation of osmotic balance and stomatal aperture mediated by increased myo-inositol biosynthesis. Taken together, our findings shed light on the positive effects of MdMIPS1-mediated myo-inositol biosynthesis on drought tolerance and WUE in apple.
Received: 26 May 2020
Accepted: 23 August 2021
|Fund: This work was supported by the National Key Research and Development Program of China (2018YFD1000303) and the China Agriculture Research System of MOF and MARA (CARS-27).
|About author: Correspondence MA Feng-wang, Tel: +86-29-87082648, E-mail: email@example.com, firstname.lastname@example.org
Cite this article:
HU Ling-yu, YUE Hong, ZHANG Jing-yun, LI Yang-tian-su, GONG Xiao-qing, ZHOU Kun, MA Feng-wang.
Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple. Journal of Integrative Agriculture, 21(7): 1968-1981.
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