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Journal of Integrative Agriculture
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Multi-omics analysis reveals that wheat enhances resistance to the English grain aphid (Sitobion avenae) through JA signaling pathway and phenylpropanoid biosynthesis pathway

Zhenyu Wang1, Weixi Hao1, Yuting Zhao1, Yaxin Guo1, Dongfu Geng1, Hao Wang1, Pingchuan Deng1, 2, Tingdong Li1, 2, Xue Shi1, 2, Changyou Wang1, 2, Jixin Zhao1, 2, Chunhuan Chen1, 2, Wanquan Ji1, 2#, Xinlun Liu1, 2#

1 College of Agronomy, Northwest A & F University, Yangling 712100, China

2 State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest Agriculture and Forestry University, Yangling 712100, China

Highlights

High-resolution temporal metabolomic profile of wheat spike post Sitobion avenae infestation.

Jasmonic acid and phenylpropanoid derivatives exhibited synergistic interactions in wheat spike reponse to Sitobion avenae infestation.

A transcriptome-metabolome roadmap for precision breeding of wheat.

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摘要  

麦长管蚜(Sitobion avenae)是小麦生产中最具破坏性的害虫之一,严重危害小麦的产量和质量。本研究评估了小麦品种轮选144(抗蚜)和济麦22(感)在成熟期的抗性。轮选144表现出更强的排趋性抗生性和耐受性,穗蚜虫较少,千粒重损失明显低于济麦22。在麦长管蚜侵染后6244872小时,轮选144和济麦22共检测到3304种代谢物。轮选144穗部产生的差异累积代谢物(662-1051)远多于济麦22336-594),其中黄酮类和酚酸类是主要差异代谢物通路富集显示,植物激素信号转导、α亚麻酸代谢、苯丙烷生物合成、黄酮类生物合成四条通路在轮选144穗部显著富集。在麦长管蚜侵食72小时轮选144穗部的关键防御代谢产物较济麦22积累到显著更高的水平:茉莉酸(JA)(2.70倍)、茉莉酸内酯(4.90倍)和总黄酮(1.40倍)。这些结果表明,轮选144通过激活JA信号和苯丙烷类生物合成途径增强了小麦对麦长管蚜的抗性,阐明了小麦对麦长管蚜抗性的代谢机制,为小麦麦长管抗性育种提供了潜在的候选代谢物。



Abstract  

The English grain aphid (Sitobion avenae) is one of the most devastating pests in wheat production, which seriously endangers the yield and quality of wheat. This study evaluated the S. avenae resistance of wheat varieties Lunxuan 144 (S. avenae-resistant) and Jimai 22 (S. avenae-susceptible) at the adult stage. Lunxuan 144 showed stronger antixenosis, antibiosis and tolerance, with fewer aphids on the spikes and lower thousand-grain weight loss than Jimai 22. A total of 3,304 metabolites were detected in wheat spikes at 6, 24, 48, and 72 h post S. avenae infestation. Lunxuan 144 produced far more differentially accumulated metabolites (DAMs, 662-1,051) than Jimai 22 (336-594), among which flavonoids and phenolic acids constituted the predominant genotype-specific DAMs. Four core metabolic pathways (plant hormone signal transduction, α-linolenic acid metabolism, phenylpropanoid biosynthesis, flavonoid biosynthesis) were more significantly enriched in the spike of Lunxuan 144. Subsequent validation revealed that key defense metabolites accumulated to significantly higher levels in Lunxuan 144 at 72 h post infestation: jasmonic acid (JA) (2.70-fold), JA-Ile (4.90-fold), and total flavonoids (1.40-fold) relative to Jimai 22. These results indicated Lunxuan 144 enhanced S. avenae resistance by activating JA signaling and phenylpropanoid biosynthesis pathways, clarifying the metabolic mechanism of S. avenae resistance in wheat and providing potential candidate metabolites for S. avenae-resistant wheat breeding.

Keywords:  Triticum aestivum       Sitobion avenae              metabolomics              JA pathway              phenylpropanoid pathway  
Received: 10 April 2026   Online: 14 September 2026  
Fund: 

This work was supported by the National Key Agricultural Science and Technology Project (NK2022060503) and the Biological Breeding-National Science and Technology Major Project (2023ZD0402501).

About author:  Zhenyu Wang, E-mail: wzhenyu2018@163.com; #Correspondence Wanquan Ji, E-mail: wqji2011@nwafu.edu.cn; Xinlun Liu, E-mail: liuxinlun@nwafu.edu.cn

Cite this article: 

Zhenyu Wang, Weixi Hao, Yuting Zhao, Yaxin Guo, Dongfu Geng, Hao Wang, Pingchuan Deng, Tingdong Li, Xue Shi, Changyou Wang, Jixin Zhao, Chunhuan Chen, Wanquan Ji, Xinlun Liu. 2026.

Multi-omics analysis reveals that wheat enhances resistance to the English grain aphid (Sitobion avenae) through JA signaling pathway and phenylpropanoid biosynthesis pathway . Journal of Integrative Agriculture, Doi:10.1016/j.jia.2026.09.020

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