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| BcSDR1 is involved in regulation of glucose transport and cAMP and MAPK signaling pathways in Botrytis cinerea |
SI He-long2*, ZHANG Kang1, 2*, LI Bai1, 2*, YUAN Xue-mei2, ZANG Jin-ping2, CAO Hong-zhe2, XING Ji-hong1, 2, DONG Jin-gao1, 2
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1 State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, P.R.China
2 Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, P.R.China
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摘要
前期研究表明，灰葡萄孢BcSDR1基因参与调控病菌的生长发育和致病过程。然而，BcSDR1的调控机制以及BcSDR1与cAMP和MAPK信号通路的关系还不是很清楚。本研究中，转录组数据显示，BcSDR1参与了葡萄糖跨膜运输、信号转导、次级代谢等生物过程。BcSDR1突变体（BCt41）对cAMP和MAPK信号通路特异性抑制剂SQ22536和U0126的敏感性非常弱，cAMP含量明显下降。进一步通过qRT-PCR 来检测cAMP和MAPK信号通路的关键基因的表达量，发现BcSDR1突变体中BcGB1、BcBTP1、BcBOS1、BcRAS1和BcBMP3明显上调，而BcPLC1、BcBCG1、BcCDC4、BcSAK1、BcATF1和BcBAP1明显下调。BcSDR1在BcBCG2、BcBCG3、BcPKA1和BcPKAR的RNAi突变体中明显上调，但在BcPKA2、BcBMP1和BcBMP3的RNAi突变体中显著下调。因此，BcBCG2、BcBCG3、BcPKA1和BcPKAR负向调节BcSDR1的表达，而BcPKA2、BcBMP1和BcBMP3正向调节BcSDR1表达。本研究可为制定持久控制灰霉病的策略提供理论依据和实践基础，同时为研究其他真菌的遗传、发育和致病性提供重要参考价值依据。
Abstract
Botrytis cinerea is a typical necrotrophic pathogenic fungus that causes severe diseases in a wide range of plant species, leading to significant economic losses. Our previous study showed that BcSDR1 positively regulates growth, development, and pathogenicity of B. cinerea. However, the regulation mechanism of BcSDR1 and the relationship between BcSDR1 and cAMP and MAPK signaling pathways are not well understood. In this study, transcriptome data showed that BcSDR1 is involved in glucose transmembrane transport, signal transduction, secondary metabolism, and other biological processes. BcSDR1 mutant (BCt41) showed remarkably weak sensitivity to cAMP and MAPK signaling pathways specific inhibitors, SQ22536 and U0126, and significantly decreased cAMP content. The key genes of cAMP and MAPK signaling pathways, BcGB1, BcBTP1, BcBOS1, BcRAS1, and BcBMP3 were significantly upregulated, whereas BcPLC1, BcBCG1, BcCDC4, BcSAK1, BcATF1, and BcBAP1 were significantly downregulated (P<0.05). BcSDR1 was obviously upregulated in BcBCG2, BcBCG3, BcPKA1, and BcPKAR RNA interference (RNAi) mutants, but significantly downregulated in BcPKA2, BcBMP1, and BcBMP3 RNAi mutants. Thus, BcBCG2, BcBCG3, BcPKA1, and BcPKAR negatively regulate BcSDR1 expression, whereas BcPKA2, BcBMP1, and BcBMP3 positively regulate BcSDR1 expression.
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Received: 25 January 2022
Accepted: 08 April 2022
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| Fund:
This study was supported by the National Natural Science Foundation of China (31972217 and 32072369), the Central Government Guides Local Science and Technology Development Projects, China (206Z6501G and 216Z6502G), and the Research Project of Basic Scientific Research Business Fees in Provincial Universities of Hebei Province, China (KY2021043 and KY2021044).
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| About author: SI He-long, E-mail: 172812997@qq.com; Correspondence ZHANG Kang, E-mail: zk7588@hebau.edu.cn; XING Ji-hong, E-mail: xingjihong2000@126.com; DONG Jin-gao, E-mail: shmdjg@hebau.edu.cn
* These authors contributed equally to this study. |
Cite this article:
SI He-long, ZHANG Kang, LI Bai, YUAN Xue-mei, ZANG Jin-ping, CAO Hong-zhe, XING Ji-hong, DONG Jin-gao.
2022.
BcSDR1 is involved in regulation of glucose transport and cAMP and MAPK signaling pathways in Botrytis cinerea. Journal of Integrative Agriculture, 21(9): 2628-2640.
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