Please wait a minute...
Journal of Integrative Agriculture  2023, Vol. 22 Issue (3): 752-761    DOI: 10.1016/j.jia.2022.08.069
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
StOFP20 regulates tuber shape and interacts with TONNEAU1 Recruiting Motif proteins in potato

AI Ju*, WANG Ye*, YAN Ya-wen, LI Chen-xiao, LUO Wei, MA Ling, SHANG Yi, GAO Dong-li# 

Yunnan Key Laboratory of Potato Biology, the CAAS-YNNU-YINMORE Joint Academy of Potato Science, Yunnan Normal University, Kunming 650500, P.R.China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      



The OVATE family proteins (OFPs) are plant-specific proteins that modulate diverse aspects of plant growth and development.  In tomato, OFP20 has been shown to interact with TONNEAU1 Recruiting Motif (TRM) proteins to regulate fruit shape.  In this study, we demonstrated that the mutation of StOFP20 caused a shift from round to oval shaped tubers in a diploid accession C151, supporting the role of StOFP20 in controlling tuber shape.  Its expression reached a maximum in the tuber initiation stage and then decreased as the tuber develops.  To help elucidate the mechanism of tuber shape regulation by StOFP20, 27 TONNEAU1 Recruiting Motif (TRM) proteins were identified and 23 of them were successfully amplified in C151.  A yeast two-hybrid assay identified three TRM proteins that interacted with StOFP20, which was confirmed by firefly luciferase complementation in tobacco leaves.  The OVATE domain was indispensable for the interactions, while the necessity of the M10 motif in TRM proteins varied among the interactions between StOFP20 and the three TRMs.  In summary, both StOFP20 and SlOFP20 directed interactions with TRM proteins, but the corresponding interactants were not completely consistent, implying that they exert regulatory roles through mechanisms that are only partially overlapping.  

Keywords:  potato       tuber shape       OFP20       TRM  
Received: 22 October 2020   Accepted: 27 December 2021
Fund: This work was supported by the National Natural Science Foundation of China (32060684) and the Academician Workstation of Yunnan, China (202105AF150028).
About author:  AI Ju, E-mail:; WANG Ye, E-mail:; #Correspondence GAO Dong-li, Tel/Fax: +86-871-65941383, E-mail: * These authors contributed equally to this study.

Cite this article: 

AI Ju, WANG Ye, YAN Ya-wen, LI Chen-xiao, LUO Wei, MA Ling, SHANG Yi, GAO Dong-li. 2023. StOFP20 regulates tuber shape and interacts with TONNEAU1 Recruiting Motif proteins in potato. Journal of Integrative Agriculture, 22(3): 752-761.

Ai J, Zhang H, Luo W, Gao D. 2020. Cloning of StOFP20 controlling tuber shape in potato. Journal of Yunnan Normal University, 40, 58–62. (in Chinese)
Alsahlany M, Zarka D, Coombs D, Douches D S. 2019. Comparison of methods to distinguish diploid and tetraploid potato in applied diploid breeding. American Journal of Potato Research, 96, 244–254.
Azimzadeh J, Nacry P, Christodoulidou A, Drevensek S, Camilleri C, Amiour N, Parcy F, Pastuglia M, Bouchez D. 2008. Arabidopsis TONNEAU1 proteins are essential for preprophase band formation and interact with centrin. Plant Cell, 20, 2146–2159.
Bailey T L, Elkan C. 1994. Fitting a mixture model by expectation maximization to discover motifs in biopolymers. Proceedings International Conference on Intelligent Systems for Molecular Biology, 2, 28–36. 
Camilleri C, Azimzadeh J, Pastuglia M, Bellini C, Grandjean O, Bouchez D. 2002. The Arabidopsis TONNEAU2 gene encodes a putative novel protein phosphatase 2A regulatory subunit essential for the control of the cortical cytoskeleton. Plant Cell, 14, 833–845. 
Drevensek S, Goussot M, Duroc Y, Christodoulidou A, Steyaert S, Schaefer E, Duvernois E, Grandjean O, Vantard M, Bouchez D, Pastuglia M. 2012. The Arabidopsis TRM1–TON1 interaction reveals a recruitment network common to plant cortical microtubule arrays and eukaryotic centrosomes. Plant Cell, 24, 178–191.
Van Eck H J, Jacobs J M, Stam P, Ton J, Stiekema W J, Jacobsen E. 1994. Multiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs. Genetics, 137, 303–309.
Kirik A, Ehrhardt D W, Kirik V. 2012. TONNEAU2/FASS regulates the geometry of microtubule nucleation and cortical array organization in interphase Arabidopsis cells. Plant Cell, 24, 1158–1170. 
Kloosterman B, Vorst O, Hall R D, Visser R G F, Bachem C W. 2005. Tuber on a chip: Differential gene expression during potato tuber development. Plant Biotechnology Journal, 3, 505–519.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549. 
Lazzaro M D, Wu S, Snouffer A, Wang Y, van der Knaap E. 2018. Plant organ shapes are regulated by protein interactions and associations with microtubules. Frontiers in Plant Science, 9, 1766.
Lee Y K, Kim G, Kim I, Park J, Kwak S, Choi G, Chung W. 2006. LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis. Development, 133, 4305–4314. 
Lindqvist-Kreuze H, Khan A, Salas E, Meiyalaghan S, Thomson S, Gomez R, Bonierbale M. 2015. Tuber shape and eye depth variation in a diploid family of Andean potatoes. BMC Genetics, 16, 57.
Liu J, Van Eck J, Cong B, Tanksley S D. 2002. A new class of regulatory genes underlying the cause of pear-shaped tomato fruit. Proceedings of the National academy of Sciences of the United States of America, 99, 13302–13306. 
Schmittgen T D, Livak K J. 2008. Analyzing real-time PCR data by the comparative CT method. Nature Protocols, 3, 1101–1108.
Spinner L, Gadeyne A, Belcram K, Goussot M, Moison M, Duroc Y, Eeckhout D, De Winne N, Schaefer E, van de Slijke E, Persiau G, Witters E, Gevaert K, de Jaeger G, Bouchez D, van Damme D, Pastuglia M. 2013. A protein phosphatase 2A complex spatially controls plant cell division. Nature Communications, 4, 177–188. 
Wang S, Chang Y, Guo J, Chen J G. 2007. Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation. The Plant Journal, 50, 858–872. 
Wang S, Chang Y, Guo J, Zeng Q, Ellis B E, Chen J G. 2011. Arabidopsis Ovate Family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development. PLoS ONE, 8, e23896. 
Wang S, Li S, Liu Q, Wu K, Zhang J, Wang S, Wang Y, Chen X, Zhang Y, Gao C, Wang F, Huang H, Fu X. 2015. The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality. Nature Genetics, 47, 949–954.
Wu S, Zhang B, Keyhaninejad N, Rodriguez G R, Kim H J, Chakrabarti M, IIIa-Berenguer E, Taitano N K, Gonzalo M J, Diaz A, Pan Y, Leisner C P, Halterman D, Buell C R, Weng Y, Jansky S H, van Eck H, Willemsen J, Monforte A J, Meulia T, et al. 2018. A common genetic mechanism underlies morphological diversity in fruits and other organs. Nature Communications, 9, 27–36.
Xiao Y, Liu D, Zhang G, Tong H, Chu C. 2017. Brassinosteroids regulate OFP1, a DLT interacting protein, to modulate plant architecture and grain morphology in rice. Frontiers in Plant Science, 8, 1698. 
Xing H, Dong L, Wang Z, Zhang H, Han C, Liu B, Wang X, Chen Q. 2014. A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biology, 14, 327. 
Xu X, Vreugdenhil D, van Lammeren A A M. 1998. Cell division and cell enlargement during potato tuber formation. Journal of Experimental Botany, 49, 573–582.
Yang C, Ma Y, He Y, Tian Z, Li J. 2018. OsOFP19 modulates plant architecture by integrating cell division pattern and brassinosteroid signaling. Plant Journal, 93, 489–501. 
Zhang H, Ai J, Luo W, Gao D. 2020. Expression and interaction analyses of potato tuber gene StOFP20. Journal of Yunnan Normal University, 40, 63–67. (in Chinese)
Zhang X, Wu J, Yu Q, Liu R, Wang Z, Sun Y. 2020. AtOFPs regulate cell elongation by modulating microtubule orientation via direct interaction with TONNEAU2. Plant Science, 292, 110405. 
Zhou S, Hu Z, Li F, Tian S, Zhu Z, Li A, Chen G. 2019. Overexpression of SlOFP20 affects floral and pollen development. Horticulture Research, 6, 125. 
[1] XIAO Yang-yang, QIAN Jia-jia, HOU Xing-liang, ZENG Lan-ting, LIU Xu, MEI Guo-guo, LIAO Yin-yin.

Diurnal emission of herbivore-induced (Z)-3-hexenyl acetate and allo-ocimene activates sweet potato defense responses to sweet potato weevils [J]. >Journal of Integrative Agriculture, 2023, 22(6): 1782-1796.

[2] LI Rui-jie, ZHAI Hong, HE Shao-zhen, ZHANG Huan, ZHAO Ning, LIU Qing-chang. A geranylgeranyl pyrophosphate synthase gene, IbGGPS, increases carotenoid contents in transgenic sweetpotato[J]. >Journal of Integrative Agriculture, 2022, 21(9): 2538-2546.
[3] ZHU Yu, YUAN Yu-han, MEI Li-ping, DING Shuang-kun, GAO Yu-chen, DU Xian-feng, GUO Li. Comparison of structural and physicochemical properties of potato protein and potato flour modified with tyrosinase[J]. >Journal of Integrative Agriculture, 2022, 21(5): 1513-1524.
[4] DONG Suo-meng, ZHOU Shao-qun. Potato late blight caused by Phytophthora infestans: From molecular interactions to integrated management strategies[J]. >Journal of Integrative Agriculture, 2022, 21(12): 3456-3466.
[5] LI Chen, LIU Xuan-xuan, ABOUELNASR Hesham, MOHAMED HAMED Arisha, KOU Meng, TANG Wei, YAN Hui, WANG Xin, WANG Xiao-xiao, ZHANG Yun-gang, LIU Ya-ju, GAO Run-fei, MA Meng, LI Qiang. Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD[J]. >Journal of Integrative Agriculture, 2022, 21(10): 2865-2875.
[6] CHEN Li-li, WANG Hao-ying, GONG Xiao-chen, ZENG Zhao-hai, XUE Xu-zhang, HU Yue-gao. Transcriptome analysis reveals effects of red and blue lightemitting diodes (LEDs) on the growth, chlorophyll fluorescence and endogenous plant hormones of potato (Solanum tuberosum L.) plantlets cultured in vitro[J]. >Journal of Integrative Agriculture, 2021, 20(11): 2914-2931.
[7] WU Jia-yu, ZHANG Yu, ZHOU Xue-ping, QIAN Ya-juan. Three sensitive and reliable serological assays for detection of potato virus A in potato plants[J]. >Journal of Integrative Agriculture, 2021, 20(11): 2966-2975.
[8] YIN Jian, CHENG Li, HONG Yan, LI Zhao-feng, LI Cai-ming, BAN Xiao-feng, GU Zheng-biao . Use of two-stage dough mixing process in improving water distribution of dough and qualities of bread made from wheat–potato flour[J]. >Journal of Integrative Agriculture, 2021, 20(1): 300-310.
[9] LIU Min-min, LI Ya-lun, LI Guang-cun, DONG Tian-tian, LIU Shi-yang, LIU Pei, WANG Qing-guo. Overexpression of StCYS1 gene enhances tolerance to salt stress in the transgenic potato (Solanum tuberosum L.) plant[J]. >Journal of Integrative Agriculture, 2020, 19(9): 2239-2246.
[10] WANG Yi-fan, LIAO Yu-qiu, WANG Ya-peng, YANG Jiang-wei, ZHANG Ning, SI Huai-jun. Genome-wide identification and expression analysis of StPP2C gene family in response to multiple stresses in potato (Solanum tuberosum L.)[J]. >Journal of Integrative Agriculture, 2020, 19(6): 1609-1624.
[11] KONG Liang, WANG Hui-bin, WANG Shuai-shuai, XU Ping-ping, ZHANG Ruo-fang, DONG Suo-meng, ZHENG Xiao-bo.
Rapid detection of potato late blight using a loop-mediated isothermal amplification assay
[J]. >Journal of Integrative Agriculture, 2020, 19(5): 1274-1282.
[12] ZHANG Yu, GAO Yan-ling, HE Wan-qin, WANG Ya-qin, QIAN Ya-juan, ZHOU Xue-ping, WU Jian-xiang .
Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers
[J]. >Journal of Integrative Agriculture, 2020, 19(5): 1283-1291.
[13] HUANG Ze-ying, Alec ZUO, SUN Jun-mao, GUO Yan-zhi. Potato farmers’ preference for agricultural insurance in China: An investigation using the choice experimental method[J]. >Journal of Integrative Agriculture, 2020, 19(4): 1137-1148.
[14] Silvia I. RONDON. Decoding Phthorimaea operculella (Lepidoptera: Gelechiidae) in the new age of change[J]. >Journal of Integrative Agriculture, 2020, 19(2): 316-324.
[15] Jessica VEREIJSSEN .
Ecology and management of Bactericera cockerelli and Candidatus Liberibacter solanacearum in New Zealand
[J]. >Journal of Integrative Agriculture, 2020, 19(2): 333-337.
No Suggested Reading articles found!