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Journal of Integrative Agriculture  2023, Vol. 22 Issue (8): 2295-2305    DOI: 10.1016/j.jia.2023.02.006
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A single nucleotide substitution in the MATE transporter gene regulates plastochron and many noded dwarf phenotype in barley (Hordeum vulgare L.)
GUO Bao-jian1, 2*, SUN Hong-wei1*, QI Jiang1, HUANG Xin-yu1, HONG Yi1, HOU Jian1LÜ Chao1, WANG Yu-lin2, WANG Fei-fei1, ZHU Juan1, GUO Gang-gang3, XU Ru-gen1
1 Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics, Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China
2 State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement/Tibet Academy of Agricultural and Animal Husbandry Sciences (TAAAS), Lhasa 850002, P.R.China
3 Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
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Abstract  In higher plants, the shoot apical meristem produces lateral organs in a regular spacing (phyllotaxy) and timing (plastochron).  The molecular analysis of mutants associated with phyllotaxy and plastochron would increase our understanding of the mechanism of shoot architecture formation.  In this study, we identified mutant mnd8ynp5 that shows an increased rate of leaf emergence and a larger number of nodes in combination with a dwarfed growth habit from an EMS-treated population of the elite barley cultivar Yangnongpi 5.  Using a map-based cloning strategy, the mnd8 gene was narrowed down to a 6.7-kb genomic interval on the long arm of chromosome 5H.  Sequence analysis revealed that a C to T single-nucleotide mutation occurred at the first exon (position 953) of HORVU5Hr1G118820, leading to an alanine (Ala) to valine (Val) substitution at the 318th amino acid site.  Next, HORVU5Hr1G118820 was defined as the candidate gene of MND8 encoding 514 amino acids and containing two multidrug and toxic compound extrusion (MATE) domains.  It is highly homologous to maize Bige1 and has a conserved function in the regulation of plant development by controlling the leaf initiation rate.  Examination of modern barely varieties showed that Hap-1 was the dominant haplotype and was selected in barley breeding around the world.  Collectively, our results indicated that mnd8ynp5 is a novel allele of the HORVU5Hr1G118820 gene that is possibly responsible for the shortened plastochron and many noded dwarf phenotype in barley.
Keywords:  barley       EMS        plastochron        many noded dwarf        MATE transporter        shoot architecture  
Received: 28 August 2022   Accepted: 10 November 2022

This work was funded by the Open Project Program of State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement, China (XZNKY-2021-C-014-K01), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (19KJA560005), the China Agriculture Research System (CARS-05), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China.

About author:  GUO Bao-jian, E-mail:; SUN Hong-wei, E-mail:; #Correspondence XU Ru-gen, Tel: +86-514-87979254, E-mail: * These authors contributed equally to this study.

Cite this article: 

GUO Bao-jian, SUN Hong-wei, QI Jiang, HUANG Xin-yu, HONG Yi, HOU Jian, LÜ Chao, WANG Yu-lin, WANG Fei-fei, ZHU Juan, GUO Gang-gang, XU Ru-gen. 2023. A single nucleotide substitution in the MATE transporter gene regulates plastochron and many noded dwarf phenotype in barley (Hordeum vulgare L.). Journal of Integrative Agriculture, 22(8): 2295-2305.

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