|Evaluation of the early defoliation trait and identification of resistance genes through a comprehensive transcriptome analysis in pears
|SHAN Yan-fei1*, LI Meng-yan1*, WANG Run-ze1, LI Xiao-gang2, LIN Jing2, LI Jia-ming1, ZHAO Ke-jiao1, WU Jun1
1 College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R.China
2 Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210095, P.R.China
Early defoliation, which usually occurs during summer in pear trees, is gradually becoming a major problem that poses a serious threat to the pear industry in southern China. However, there is no system for evaluating the responses of different cultivars to early defoliation, and our knowledge of the potential molecular regulation of the genes underlying this phenomenon is still limited. In this study, we conducted field investigations of 155 pear accessions to assess their resistance or susceptibility to early defoliation. A total of 126 accessions were found to be susceptible to early defoliation, and only 29 accessions were resistant. Among them, 19 resistant accessions belong to the sand pear species (Pyrus pyrifolia). To identify the resistance genes related to early defoliation, the healthy and diseased samples of two sand pear accessions, namely, the resistant early defoliation accession ‘Whasan’ and the susceptible early defoliation accession ‘Cuiguan’, were used to perform RNA sequencing. Compared with ‘Cuiguan’, a total of 444 genes were uniquely differentially expressed in ‘Whasan’. Combined with GO and KEGG enrichment analyses, we found that early defoliation was closely related to the stress response. Furthermore, a weighted gene co-expression network analysis revealed a high correlation of WRKY and ethylene responsive factor (ERF) transcription factors with early defoliation resistance. This study provides useful resistant germplasm resources and new insights into potentially essential genes that respond to early defoliation in pears, which may facilitate a better understanding of the resistance mechanism and molecular breeding of resistant pear cultivars
Received: 24 March 2021
Accepted: 21 April 2022
This work was supported by the earmarked fund for Jiangsu Agricultural Industry Technology System, China (JATS 453), the National Key Research and Development Program of China (2021YFD1200200), and the earmarked fund for China Agriculture Research System (CARS-28). We thank the State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, and the Bioinformatics Center of Nanjing Agricultural University, China for supporting this project.
|About author: SHAN Yan-fei, E-mail: firstname.lastname@example.org; LI Meng-yan, E-mail: email@example.com; Correspondence WU Jun, E-mail: firstname.lastname@example.org
* These authors contributed equally to this study.
Cite this article:
SHAN Yan-fei, LI Meng-yan, WANG Run-ze, LI Xiao-gang, LIN Jing, LI Jia-ming, ZHAO Ke-jiao, WU Jun.
Evaluation of the early defoliation trait and identification of resistance genes through a comprehensive transcriptome analysis in pears. Journal of Integrative Agriculture, 22(1): 120-138.
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