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Journal of Integrative Agriculture  2020, Vol. 19 Issue (10): 2561-2570    DOI: 10.1016/S2095-3119(20)63201-4
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
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Contrasting resilience of soil microbial biomass, microbial diversity and ammonification enzymes under three applied soil fumigants
SUN Zhen-cai1, LI Gui-tong2, ZHANG Cheng-lei3, WANG Zhi-min1, LIN Qi-mei2, ZHAO Xiao-rong2
1 College of Agronomy, China Agricultural University, Beijing 100193, P.R.China
2 College of Land Science and Technology, China Agricultural University, Beijing 100193, P.R.China
3 Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, P.R.China
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Fumigation is a widely applied approach to mitigate the soil-borne diseases.  However, the potential effects of currently applied fumigants on ammonification remain unclear.  An 84-day incubation experiment was conducted based on non-fumigated soil (CK) and fumigated soil using three common fumigants, i.e., chloropicrin (CP), 1,3-dichloropropene (1,3-D), and metam sodium (MS).  The results showed that, the three fumigants all decreased the microbial C, and the largest reduction (84.7%) occurred with the application of CP.  After fumigation, the microbial diversity in the CP treatment rapidly recovered, but that in the 1,3-D treatment decreased and did not recover by the end of the experiment.  The application of MS showed no impact on the microbial diversity during the assay, indicating that significantly different microbial diversity can be achieved by choosing different fumigants.  Furthermore, the three fumigants showed divergent effects on the enzymes involved in ammonification.  The analysis showed that the enzyme variation with CP application was mainly associated with the changed microbial C and N (P<0.05), and not with the microbial community, which was different from the observed effects of 1,3-D or MS application.  In addition, the soil quality index showed that CP was still significantly harmful at the end of incubation compared with the good resilience of MS, indicating that CP may not be a suitable fumigant.
Keywords:  chloropicrin        1,3-dichloropropene        metam sodium        N cycling        microbial biomass and diversity  
Received: 21 October 2019   Accepted:
Fund: This work was supported by the National Key Research and Development Program of China (2018YFD0300505) and the National Key Technologies Project of China in Urban Agriculture during the12th Five-Year Plan (2014BAD14B03).
Corresponding Authors:  Correspondence LI Gui-tong, E-mail:   
About author:  SUN Zhen-cai, E-mail:;

Cite this article: 

SUN Zhen-cai, LI Gui-tong, ZHANG Cheng-lei, WANG Zhi-min, LIN Qi-mei, ZHAO Xiao-rong. 2020. Contrasting resilience of soil microbial biomass, microbial diversity and ammonification enzymes under three applied soil fumigants. Journal of Integrative Agriculture, 19(10): 2561-2570.

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