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Journal of Integrative Agriculture  2019, Vol. 18 Issue (6): 1280-1294    DOI: 10.1016/S2095-3119(19)62571-2
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Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress
GENG Da-li1, LU Li-yuan1, YAN Ming-jia1, SHEN Xiao-xia1, JIANG Li-juan1, LI Hai-yan1, WANG Li-ping1, YAN Yan1, XU Ji-di1, LI Cui-ying1, YU Jian-tao2, MA Feng-wang1, GUAN Qing-mei  
1 State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
2 College of Information Engineering, Northwest A&F University, Yangling 712100, P.R.China
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Water deficit is one of the main limiting factors for apple growth and production.  Root architecture plays an important role in drought tolerance of plants.  However, little is known about the molecular basis of root system in apple trees under drought.  In this study, we compared root morphology of two widely used rootstocks of apple (R3 and Malus sieversii) under drought.  Our results suggested that M. sieversii is more tolerant to drought than R3, since M. sieversii had a higher ratio of root to shoot as well as root hydraulic conductivity under long-term drought conditions.  We then performed whole-genome transcriptomic analysis to figure out the molecular basis of root responses in M. sieversii under drought.  It was found that genes involved in transcription regulation, signaling or biosynthesis of hormones, and oxidative stress were differentially expressed under drought.  Consistent with the gene expression profile, roots of M. sieversii had higher activities of peroxidase (POD) and superoxide dismutase (SOD) under drought, as well as higher content of abscisic acid (ABA) and lower content of auxin.  Taken together, our results revealed the physiological and transcriptomic analyses of M. sieversii roots in response to drought. 
Keywords:  Malus sieversii        root architecture        drought stress        RNA-seq  
Received: 30 May 2018   Accepted:
Fund: This work was supported by the National Natural Science Foundation of China (31622049 and 31572106), the Key Program of the National Natural Science Foundation of China (31330068), the Program of Sci-Tech Star of Shaanxi, China (2015kjxx14), the startup funding (Z111021402) from Northwest A&F University to Guan Qingmei who is also supported by the Thousand Talents Plan of China.
Corresponding Authors:  Correspondence MA Feng-wang, Tel/Fax: +86-29-87082648, E-mail:; GUAN Qing-mei, Tel/Fax: +86-29-87082648, E-mail:   
About author:  GENG Da-li, E-mail:;

Cite this article: 

GENG Da-li, LU Li-yuan, YAN Ming-jia, SHEN Xiao-xia, JIANG Li-juan, LI Hai-yan, WANG Li-ping, YAN Yan, XU Ji-di, LI Cui-ying, YU Jian-tao, MA Feng-wang, GUAN Qing-mei. 2019. Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress. Journal of Integrative Agriculture, 18(6): 1280-1294.

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