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Journal of Integrative Agriculture  2020, Vol. 19 Issue (1): 62-77    DOI: 10.1016/S2095-3119(19)62632-8
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Effects of uniconazole with or without micronutrient on the lignin biosynthesis, lodging resistance, and winter wheat production in semiarid regions
Irshad AHMAD1, 2*, MENG Xiang-ping1, 2*, Muhammad KAMRAN1, 2, Shahzad ALI1, 2, Shakeel AHMAD1, 2, LIU Tie-ning1, 2, CAI Tie1, 2, HAN Qing-fang1, 2   
1 Key Laboratory of Crop Physio-Ecology and Tillage Science in North-Western Loess Plateau, Ministry of Agriculture/College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
2 Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education/Institute of Water Saving Agriculture in Arid Areas of China, Northwest A&F University, Yangling 712100, P.R.China
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Lodging stress results in grain yield and quality reduction in wheat.  Uniconazole, a potential plant growth regulator significantly enhances lignin biosynthesis and thus provides mechanical strength to plants in order to cope with lodging stress.  A field study was conducted during the 2015–2016 and 2016–2017 growing seasons, to investigate the effects of uniconazole sole application or with micronutrient on the lignin biosynthesis, lodging resistance, and production of winter wheat.  In the first experiment, uniconazole at concentrations of 0 (CK), 15 (US1), 30 (US2), and 45 (US3) mg L–1 was applied as sole seed soaking, while in the second experiment with manganese (Mn) at concentration of 0.06 g L–1 Mn, 0.06 g L–1 Mn+15 mg L–1 uniconazole (UMS1), 0.06 g L–1 Mn+30 mg L–1 uniconazole (UMS2), and 0.06 g L–1 Mn+45 mg L–1 uniconazole (UMS3), respectively.  Uniconazole sole application or with micronutrient significantly increased the lignin content by improving the lignin-related enzyme activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, tyrosine ammonia-lyase, and peroxidase, ameliorating basal internode characteristics, and breaking strength.  The spike length, spike diameter, spikes/plant, weight/spike, yield/spike, and grain yield increased and then decreased with uniconazole application at a higher concentration, where their maximum values were recorded with UMS2 and US2 treatments.  The lignin accumulation was positively correlated with lignin-related enzyme activities and breaking strength while, negatively correlated with lodging rate.  Uniconazole significantly improved the lignin biosynthesis, lodging resistance, and grain yield of winter wheat and the treatments which showed the greatest effects were uniconazole seed soaking with micronutrient at a concentration of 30 mg L–1 and 0.06 g L–1, and uniconazole sole seed soaking at a concentration of 30 mg L–1.
Keywords:  lignin biosynthesis        lodging resistance        semi-arid regions        manganese        uniconazole        wheat  
Received: 30 August 2018   Accepted:
Fund: This study was supported by funding from the National High-Tech R&D Program of China (2013AA102902), the Program of Introducing Talents of Discipline to Universities, China (B12007), and the National Natural Science Foundation of China (31601257).
Corresponding Authors:  Correspondence CAI Tie, E-mail:; HAN Qing-fang, E-mail:    
About author:  * These authors contributed equally to this study.

Cite this article: 

Irshad AHMAD, MENG Xiang-ping, Muhammad KAMRAN, Shahzad ALI, Shakeel AHMAD, LIU Tie-ning, CAI Tie, HAN Qing-fang . 2020. Effects of uniconazole with or without micronutrient on the lignin biosynthesis, lodging resistance, and winter wheat production in semiarid regions. Journal of Integrative Agriculture, 19(1): 62-77.

Acreche M M, Briceño-Félix G, Martín Sanchez J A, Slafer G A. 2008. Physiological bases of genetic gains in Mediterranean bread wheat yield in Spain. European Journal of Agronomy, 28, 162–170.
Acreche M M, Slafer G A. 2011. Lodging yield penalties as affected by breeding in Mediterranean wheats. Field Crops Research, 122, 40–48.
Ahmad I, Kamran M, Ali S, Bilegjargal B, Cai T, Ahmad S, Meng X P, Su W N, Liu T, Han Q F. 2018a. Uniconazole application strategies to improve lignin biosynthesis, lodging resistance and production of maize in semiarid regions. Field Crops Research, 222, 66–77.
Ahmad I, Kamran M, Ali S, Cai T, Bilegjargal B, Liu T, Han Q F. 2018b. Seed filling in maize and hormones crosstalk regulated by exogenous application of uniconazole in semiarid regions. Environmental Science and Pollution Research, 25, 33225–33239.
Ahmad I, Kamran M, Su W N, Haiqi W, Ali S, Bilegjargal B, Ahmad S, Liu T, Cai T, Han Q F. 2019. Application of uniconazole improves photosynthetic efficiency of maize by enhancing the antioxidant defense mechanism and delaying leaf senescence in semiarid regions. Journal of Plant Growth Regulation, 38, 855–869.
Barros J, Serk H, Granlund I, Pesquet E. 2015. The cell biology of lignification in higher plants. Annals of Botany, 115, 1053–1074.
Berry P M, Spink J. 2012. Predicting yield losses caused by lodging in wheat. Field Crops Research, 137, 19–26.
Berry P M, Spink J H, Gay A P, Craigon J. 2003. A comparison of root and stem lodging risks among winter wheat cultivars. Journal of Agricultural Science, 141, 191–202.
Boudet A M, Kajita S, Grima-Pettenati J, Goffner D. 2003. Lignins and lignocellulosics: A better control of synthesis for new and improved uses. Trends in Plant Science, 8, 576–581.
Campbell M M, Sederoff R R. 1996. Variation in lignin content and composition (mechanisms of control and implications for the genetic improvement of plants). Plant Physiology, 110, 3–13.
Chen X G, Shi C Y, Yin Y P, Wang Z L, Shi Y H, Peng D L, Ni Y L, Cai T. 2011. Relationship between lignin metabolism and lodging resistance in wheat. Acta Agronomica Sinica, 37, 1616–1622. (in Chinese)
Esechie H A, Rodriguez V, Al-Asmi H. 2004. Comparison of local and exotic maize varieties for stalk lodging components in a desert climate. European Journal of Agronomy, 21, 21–30.
FAO (Food and Agriculture Organization). 2016. Foreign Agricultural Service, United States Department of Agriculture, Office of Global Analysis. [2016-06-15].
Fang X, Liu X, Zhang Y, Huang K, Zhang Y, Li Y, Nie J, She H, Ruan R, Yuan X, Yi Z. 2018. Effects of uniconazole or gibberellic acid application on the lignin metabolism in relation to lodging resistance of culm in common buckwheat (Fagopyrum esculentum M.). Journal of Agronomy and Crop Science, 204, 414–423.
Fletcher R A, Hofstra G. 1990. Improvement of uniconazole-induced protection in wheat seedlings. Journal of Plant Growth Regulation, 9, 207–212.
Goodman A M, Crook M J, Ennos A R. 2001. Anchorage mechanics of the tap root system of winter sown oilseed rape (Brassica napus L.). Annals of Botany, 87, 397–404.
Grassini P, Yang H, Irmak S, Thorburn J, Burr C, Cassman K G. 2011. High-yield irrigated maize in the Western U.S. Corn Belt: II. Irrigation management and crop water productivity. Field Crops Research, 120, 133–141.
Han H, Yang W. 2009. Influence of uniconazole and plant density on nitrogen content and grain quality in winter wheat in South China. Plant Soil & Environment, 55, 159–166.
Hegazi A M, El-Shraiy A M. 2007. Impact of salicylic acid and paclobutrazol exogenous application on the growth, yield and nodule formation of common bean. Australian Journal of Basic and Applied Science, 1, 834–840.
Hubert B, Rosengrant M, van Boekel M, Ortiz R. 2010. The future of food: Scenarios for 2050. Crop Science, 50, S33–S50.
Hussein M M, Bakheta M A, Zaki S N S. 2014. Influence of uniconazole on growth characters, photosynthetic pigments, total carbohydrates and total soluble sugars of Hordium vulgare L. plants grown under salinity stress. International Journal of Science and Research, 3, 2208–2214.
Ipekci Z, Ogras T, Altinkut A, Bajrovic K, Kazan K, Gozukirmizi N, Boydak M, Tank T, Akalp T, Ozden O. 1999. Reduced leaf peroxidase activity is associated with reduced lignin content in transgenic poplar. Plant Tissue Culture Letters, 16, 381–387.
Jones L, Ennos A R, Turner S R. 2001. Cloning and characterization of irregular xylem4 (irx4): A severely lignin-deficient mutant of Arabidopsis. The Plant Journal, 26, 205–216.
Kamran M, Ahmad I, Wang H Q, Wu X R, Xu J, Tiening L, Ding R X, Han Q F. 2018a. Mepiquat chloride application increases lodging resistance of maize by enhancing stem physical strength and lignin biosynthesis. Field Crops Research, 224, 148–159.
Kamran M, Cui W, Ahmad I, Meng X P, Zhang X, Su W, Chen J, Ahmad S, Fahad S, Han Q F, Liu T. 2018b. Effect of paclobutrazol, a potential growth regulator on stalk mechanical strength, lignin accumulation and its relation with lodging resistance of maize. Plant Growth Regulation, 84, 317–332.
Kang M S, Din A K, Zhang Y, Magari R. 1999. Combining ability for rind-puncture resistance in maize. Crop Science, 39, 368–371.
Kashiwagi T, Ishimaru K. 2004. Identification and functional analysis of a locus for improvement of lodging resistance in rice. Plant Physiology, 134, 676–683.
Klapheck S, Zimmer I, Cosse H. 1990. Scavenging of hydrogen peroxide in the endosperm of ricinus communis by ascorbate peroxidase. Plant Cell Physiology, 31, 1005–1013.
Kong E, Liu D, Guo X, Yang W, Sun J, Li X, Zhan K, Cui D, Lin J, Zhang A. 2013. Anatomical and chemical characteristics associated with lodging resistance in wheat. The Crop Journal, 1, 43–49.
Li X J, Li S Y, Lin J X. 2003. Effect of GA3 spraying on lignin and auxin contents and the correlated enzyme activities in barberry (Myrica ruba Bieb.) during flower-bud induction. Plant Science, 164, 549–556.
Ma J F, Yamaji N. 2006. Silicon uptake and accumulation in higher plants. Trends in Plant Science, 11, 392–397.
Martinez-Vazquez P. 2017. Crop lodging induced by wind and rain. Agricultural and Forest Meteorology, 228, 266–275.
Meng X P, Chunxia Li, Guo H, Ding R, Yang B, Cai T, Han Q F. 2016. Effects of manganese soaking on wheat seedling photosynthetic characteristics and root system, root vigor. Acta Botanica Boreali-Occidentalia Sinica, 36, 745–750. (in Chinese)
Noor R B M, Caviness C E. 1980. Influence of induced lodging on pod distribution and seed yield in soybeans. Agronomy Journal, 72, 904–906.
Norberg O S, Mason S C, Lowry S R. 1988. Ethephon influence on harvestable yield, grain quality, and lodging of corn. Agronomy Journal, 80, 768–772.
Okuno A, Hirano K, Asano K, Takase W, Masuda R, Morinaka Y, Ueguchitanaka M, Kitano H, Matsuoka M. 2014. New approach to increasing rice lodging resistance and biomass yield through the use of high gibberellin producing varieties. PLoS ONE, 9, e86870.
Önnerud H, Zhang L, Gellerstedt G, Henriksson G. 2002. Polymerization of monolignols by redox shuttle-mediated enzymatic oxidation: A new model in lignin biosynthesis I. The Plant Cell, 14, 1953–1962.
Padhye S R, Groninger J K. 2009. Influence of benzyladenine, trinexapac-ethyl, or uniconazole applications on height and tillering of six ornamental grasses. Horttechnology, 19, 737–742.
Parry M, Lowe J, Hanson C. 2009. Overshoot, adapt and recover. Nature, 458, 1102–1103.
Peng D, Chen X, Yin Y, Lu K, Yang W, Tang Y, Wang Z. 2014. Lodging resistance of winter wheat (Triticum aestivum L.): Lignin accumulation and its related enzymes activities due to the application of paclobutrazol or gibberellin acid. Field Crops Research, 157, 1–7.
Rengel Z, Graham R D, Pedler J F. 1993. Manganese nutrition and accumulation of phenolics and lignin as related to differential resistance of wheat genotypes to the take-all fungus. Plant and Soil, 151, 255–263.
Schluttenhofer C M, Massa G D, Mitchell C A. 2011. Use of uniconazole to control plant height for an industrial/pharmaceutical maize platform. Industrial Crops & Products, 33, 720–726.
Setter T L, Laureles E V, Mazaredo A M. 1997. Lodging reduces yield of rice by self-shading and reductions in canopy photosynthesis. Field Crops Research, 49, 95–106.
Sewalt V, Ni W, Blount J W, Jung H G, Masoud S A, Howles P A, Lamb C, Dixon R A. 1997. Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonia-lyase or cinnamate 4-hydroxylase. Plant Physiology, 115, 41–50.
Slafer G A, Araus J L. 2007. Physiological traits for improving wheat yield under a wide range of conditions. In: Spiertz P C S, van Laar H H, eds., Scale and Complexity in Plant Systems Research: Gene-Plant-Crop Relations. Springer Dordrecht, The Netherlands. pp. 147–156.
Soleymani A, Hoodagi M, Shahrajabian M H. 2011. The influence of manganese sulfate on yield and yield components of three wheat cultivars in Abadeh region. Research on Crops, 9, 247–248.
Song S, Feng N J, Zheng D F. 2008. Effect of seed soaking with uniconazole on germination and anti-oxidant enzyme of soybean. Soybean Science, 27, 259–250. (in Chinese)
Stapper M, Fischer R A. 1990. Genotype, sowing date and plant spacing influence on high-yielding irrigated wheat in Southern New South Wales. II. Growth, yield and nitrogen use. Australian Journal of Agricultural Research, 41, 1021–1041.
Tanaka K, Murata K, Yamazaki M, Onosato K, Miyao A, Hirochika H. 2003. Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall. Plant Physiology, 133, 73–83.
Tripathi S C, Sayre K D, Kaul J N, Narang R S. 2003. Growth and morphology of spring wheat (Triticum aestivum L.) culms and their association with lodging: Effects of genotypes, N levels and ethephon. Field Crops Research, 84, 271–290.
Updegraff D M. 1969. Semimicro determination of cellulose in biological materials. Analytical Biochemistry, 32, 420–424.
Vera C L, Duguid S D, Fox S L, Rashid K Y, Dribnenki J C P, Clarke F R. 2012. Short communication: Comparative effect of lodging on seed yield of flax and wheat. Canadian Journal of Plant Science, 92, 39–43.
Wang C, Hu D, Liu X, She H, Ruan R, Yang H, Yi Z, Wu D. 2015. Effects of uniconazole on the lignin metabolism and lodging resistance of culm in common buckwheat (Fagopyrum esculentum M.). Field Crops Research, 180, 46–53.
Wang C, Ruan R, Yuan X, Hu D, Yang H, Li Y, Yi Z. 2014. Relationship between lignin metabolism and lodging resistance of culm in buckwheat. Jouranl of Agricultural Science (Toronto), 6, 29–36.
Wu W, Ma B L. 2016. A new method for assessing plant lodging and the impact of management options on lodging in canola crop production. Scientific Reports, 6, 31890.
Xu C, Gao Y, Tian B, Ren J, Meng Q, Wang P. 2017. Effects of EDAH, a novel plant growth regulator, on mechanical strength, stalk vascular bundles and grain yield of summer maize at high densities. Field Crops Research, 200, 71–79.
Yan Y H, Gong W Z, Yang W Y, Wan Y, Chen X L, Chen Z Q, Wang L Y. 2010. Seed treatment with uniconazole powder improves soybean seedling growth under shading by corn in relay strip intercropping system. Plant Production Science, 13, 367–374.
Ye D L, Zhang Y S, Alkaisi M M, Duan L S, Zhang M C, LI Z H. 2016. Ethephon improved stalk strength associated with summer maize adaptations to environments differing in nitrogen availability in the North China Plain. Journal of Agricultural Science, 154, 960–977.
Zhang G, Chen J, Bull D A. 2001. The effects of timing of N application and plant growth regulators on morphogenesis and yield formation in wheat. Plant Growth Regulation, 35, 239–245.
Zhang Q, Zhang L, Evers J, Werf W V D, Zhang W, Duan L. 2014. Maize yield and quality in response to plant density and application of a novel plant growth regulator. Field Crops Research, 164, 82–89.
Zheng M, Chen J, Shi Y, Li Y, Yin Y, Yang D, Luo Y, Pang D, Xu X, Li W, Ni J, Wang Y, Wang Z, Li Y. 2017. Manipulation of lignin metabolism by plant densities and its relationship with lodging resistance in wheat. Scientific Reports, 7, 41805.
Zhou W, Ye Q. 1996. Physiological and yield effects of uniconazole on winter rape (Brassica napus L.). Journal of Plant Growth Regulation, 15, 69–73.
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