|
|
|
|
|
| Streptomyces sp. RP1A-12 mediated control of peanut stem rot caused by Sclerotium rolfsii |
| Simi Jacob1, 2, Ramgopal Rao Sajjalaguddam2, 3, Hari Kishan Sudini1 |
1 International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, India
2 Jawaharlal Nehru Technological University, Kukatpally, Hyderabad 500085, India
3 Biocon Academy, Biocon House, Bangalore 560100, India |
|
|
|
|
Abstract Sclerotium rolfsii Sacc. is a destructive soilborne fungal pathogen with a wide host range that includes peanuts. Biological control offers an interesting alternative to fungicides for sustainable management of soilborne diseases. The current investigation is aimed at evaluating one potential biocontrol agent Streptomyces sp. RP1A-12 for growth promotion and the management of peanut stem rot disease caused by S. rolfsii under field conditions. Preliminary studies conducted under in vitro and the greenhouse conditions showed promising results against the stem rot pathogen. Further in vitro and pot experiments conducted to assess Streptomyces sp. RP1A-12 for its growth promoting abilities using whole organisms have shown an increase in seed germination, root and shoot length. Other parameters like nodule number and plant biomass were also significantly increased over control treatments indicating that the test bioagent possesses growth promoting abilities along with disease suppression capabilities. Subsequently field studies were carried out for two consecutive rainy seasons. The bioagent was applied as whole organism and partially purified crude metabolites. Results indicate the bioagent reduced stem rot disease incidence by 64–67% and 22–49% respectively in two field trials conducted with notable increase in yield. Partially purified Streptomyces sp. RP1A-12 metabolites exhibited an even greater effect in reducing the incidence and severity of stem rot compared to the pathogen inoculated control.
|
|
Received: 09 July 2017
Accepted:
|
| Fund: The authors are thankful to Biotechnology Industry Research Assistance Council (BIRAC), a government of India enterprise, for providing financial support under Biotechnology Industry Partnership Programme (BIPP) (BT/BIPP0429/11/10). |
|
Corresponding Authors:
Correspondence Hari Kishan Sudini, E-mail: h.sudini@cgiar.org
|
Cite this article:
Simi Jacob, Ramgopal Rao Sajjalaguddam, Hari Kishan Sudini.
2018.
Streptomyces sp. RP1A-12 mediated control of peanut stem rot caused by Sclerotium rolfsii. Journal of Integrative Agriculture, 17(04): 892-900.
|
| Adhilakshmi M, Latha P, Paranidharan V, Balachandar D, Ganesamurthy K, Velazhahan R. 2014. Biological control of stem rot of groundnut (Arachis hypogaea L.) caused by Sclerotium rolfsii Sacc. with actinomycetes. Archives of Phytopathology and Plant Protection, 47, 298–311.Alabouvette C, Olivain C, Steinberg C. 2006. Biological control of plant diseases: The European situation. European Journal of Plant Pathology, 114, 329–341.Ames R N, Reid C P, Ingham E R. 1984. Rhizosphere bacterial population responses to root colonization by a vesicular-arbuscular mycorrhizal fungus. New Phytologist, 96, 555–563.Aycock R. 1966. Stem rot and other diseases caused by Sclerotium rolfsii. Technical Bulletin, North Carolina Agricultural Experiment Station, 174, 202.Backman P A, Brenneman T B. 1997. Stem rot. In: Kokalis-Burelle N, Porter D M, Rodriguez-Kabana R, Smith D H, Subrahmanyam P, eds., Compendium of Peanut Diseases. 2nd edition. APS Press, St. Paul, Minn, USA. pp. 36–37. Bale J S, van Lenteren J C, Bigler F. 2008. Biological control and sustainable food production. Philosophical Transactions of the Royal Society (B), 363, 761–776.Basha T S, Radhaiah A, Nagalakshmi D M, Eswara R N P. 2012. Biocontrol potential of indigenous Pseudomonas spp. against Sclerotium rolfsii causing stem rot of groundnut. International Journal of Food Agriculture and Veterinary Science, 2, 134–141.Basilio A, Gonzalez I, Vicente M F, Gorrochategui J, Cabello A, González A, Genilloud O. 2003. Patterns of antimicrobial activities from soil actinomycetes isolated under different conditions of pH and salinity. Journal of Applied Microbiology, 95, 814–823.Behal V. 2000. Bioactive products from Streptomyces. Advances in Applied Microbiology, 47, 113–157.Bentley S D, Chater K F, Cerdeño-Tárraga A M, Challis G L, Thomson N R, James K D, Harris D E, Quail M A, Kieser H, Harper D, Bateman A, Brown S, Chandra G, Chen C W, Collins M, Cronin A, Fraser A, Goble A, Hidalgo J, Hornsby T, et al. 2002. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3 (2). Nature, 417, 141–147.Berdy J. 2012. Thoughts and facts about antibiotics: where we are now and where we are heading. Journal of Antibiotics, 65, 385–395.Bloemberg G V, Lugtenberg B J J. 2001. Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Current Opinion in Plant Biology, 4, 343–352.Campbell R. 1986. The search for biological control agents against plant pathogens: A pragmatic approach. Biological Agriculture and Horticulture, 3, 317–327.Chamblee D S, Green J T. 1995. Ragdoll test for seed germination. Appendix F. In: Production and Utilization of Pastures and Forages in North Carolina. North Carolina Agricultural Research Service Technical Bulletin No. 305. p. 153.Cilliers A J, Pretorius Z A, van Wyk P S. 2003. Integrated control of Sclerotium rolfsii on groundnut in South Africa. Journal of Phytopathology, 151, 249–258.Clark C A, Moyer J W. 1988. Compendium of Sweet Potato Diseases. American Phytopathological Society, Minnesota, USA.Cook R, Baker K F. 1983. The Nature and Practice of Biological Control of Plant Pathogens. American Phytopathological Society, St Paul, Minnesota. p. 539.Cook R J. 1993. Making greater use of introduced microorganisms for biological control of plant pathogens. Annual Review of Phytopathology, 31, 53–80.Copping L G, Menn J J. 2000. Biopesticides: A review of their action, applications and efficacy. Pesticide Management Science, 56, 651–676.Dey R, Pal K K, Bhatt D M, Chauhan S M. 2004. Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiological Research, 159, 371–394.Dochhil H, Dkhar M S, Barman D. 2013. Seed germination enhancing activity of endophytic Streptomyces isolated from indigenous ethno-medicinal plant Centella Asiatica. International Journal of Pharma and BioSciences, 4, 256–262.Doumbou C L, Salove M K H, Crawford D L, Beaulieu C. 2001. Actinomycetes, promising tools to control plant diseases and to promote plant growth. Phytoprotection, 82, 85–102.Elliot L F, Lynch J M. 1995. The international workshop on establishment of Microbial inocula in soils: Cooperative research project on biological resource management of the Organization for Economic Cooperation and Development (OECD). American Journal of Alternative Agriculture, 10, 50–73.Ensign J C. 1992. Introduction to the actinomycetes. In: Balows A, Trijper H G, Dworkin M, Harder W, Schleifer K H, eds., The Prokaryotes. Springer-Verlag, New York. pp. 811–815.Franco-Correa M, Quintana A, Duque C, Suarez C, Rodríguez M X, Barea J M. 2010. Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Applied Soil Ecology, 45, 209–217.Ganesan S, Kuppusamy R G, Sekar R. 2007. Integrated management of stem rot disease (Sclerotium rolfsii) of groundnut (Arachis hypogaea L.) using Rhizobium and Trichoderma harzianum (ITCC-4572). Turkish Journal of Agriculture and Forestry, 31, 103–108.Garrido G, Guerrero J, Cano E, Acosta M, Sánchez-Bravo J. 2002. Origin and basipetal transport of the IAA responsible for rooting of carnation cuttings. Physiologia Plantarum, 114, 303–312.Gopalakrishnan S, Pande S, Sharma M, Humayun P, Kiran B K, Sandeep D, Vidya M S, Deepthi K, Rupela O M. 2011. Evaluation of actinomycete isolates obtained from herbal vermicompost for the biological control of Fusarium wilt of chickpea. Crop Protection, 30, 1–9.Haas K, Keel C, Laville J, Maurhofer M, Oberhansli T F, Schnider U, Voisard C, Wuthrich B, Defago G. 1991. Secondary metabolites of Pseudomonas fluorescens strain CHAO involved in the suppression of root diseases. In: Hennecks H, Verma D P S, eds., Advances in Molecular Genetics of Plant-Microbe Interactions. Interlaken, Switzerland. pp. 450–456.Halder A K, Mishra A K, Chakarbarthy P K. 1991. Solubilization of inorganic phosphates by Bradyrhizobium. Indian Journal of Experimental Biology, 29, 28–31.Ika R S, Syamsuddin D, Nasir S, Anton M. 2011. Control of damping off disease caused by Sclerotium rolfsii Sacc.using actinomycetes and VAM fungi on soybean in the dry land based on microorganism diversity of rhizosphere zone. Agrivita, 33, 140–146.Ismet A, Vikineswary S, Paramaswari S, Wong W H, Ward A, Seki T, Fiedler H P, Goodfellow M. 2004. Production and chemical characterization of antifungal metabolites from Micromonospora sp. M39 isolated from mangrove rhizosphere soil. World Journal of Microbiology and Biotechnology, 20, 523–528.Jiang Y, Li W J, Xu P, Tang S K, Xu L H. 2006. Study on Actinomycetes diversity under salt and alkaline environments. Acta Microbiologica Sinica, 46, 191–195. (in Chinese)Karthikeyan V, Sankaralingam A, Nakkeeran S. 2006. Biological control of groundnut stem rot caused by Sclerotium rolfsii (Sacc.). Archives of Phytopathology and Plant Protection, 39, 239–246.Kim B S, Moon S S, Hwang B K. 2000. Structure elucidation and antifungal activity of an anthracycline antibiotic, daunomycin, isolated from Actinomadura roseola. Journal of Agricultural and Food Chemistry, 48, 1875–1881.Koike S T. 2004. Southern blight of Jerusalem artichoke caused by Sclerotium rolfsii in California. Plant Disease, 88, 769.Kokalis-Burelle N, Backman P A, Rodriguez-Kabana R, Ploper L D. 1992. Potential for biological control of early leafspot of peanut using Bacillus cereus and chitin as foliar amendments. Biological Control, 2, 321–328.Lazzarini A, Cavaletti L, Toppo G, Marinelli F. 2000. Rare genera of actinomycetes as potential producers of new antibiotics. Antonie van Leewenhoek, 78, 399–405.López J, Acosta M, Sánchez-Bravo J. 2004. Role of basipetal auxin transport and lateral auxin movement in rooting and growth of etiolated lupin hypocotyl. Physiologia Plantarum, 121, 294–304.Madi L, Katan T, Katan J, Henis Y. 1997. Biological control of Sclerotium rolfsii and Verticillium dahliae by Talaromyces flavus is mediated by different mechanisms. Phytopathology, 87, 1054–1060.Manulis S, Shafir H, Epstein E, Lichter A, Barash I. 1994. Biosynthesis of indole-3-actetic acid via the indole-3-acetamide pathway in Streptomyces sp. Microbiology, 140, 1045–1050.Marciano P, Lenna P D, Magro P. 1983. Oxalic acid, cell wall-degrading enzymes and pH in pathogenesis and their significance in the virulence of two Sclerotinia sclerotiorum isolates on sunflower. Physiological Plant Pathology, 22, 339–345.Mayee C D, Datar V V. 1988. Diseases of groundnut in the tropics. Review of Tropical Plant Pathology, 5, 85–118.Meschke H, Walter S, Schrempf H. 2012. Characterization and localization of prodiginines from Streptomyces lividans suppressing Verticillium dahliae in the absence or presence of Arabidopsis thaliana. Environmental Microbiology, 14, 940–592.Ozgonen H, Akgul D S, Erkilic A. 2010. The effects of arbuscular mycorrhizal fungi on yield and stem rot caused by Sclerotium rolfsii Sacc. in peanut. African Journal of Agricultural Research, 5, 128–132.Pathom-aree W, Stach James E M, Ward A, Horikoshi K, Bull A T, Goodfellow M. 2006. Diversity of actinomycetes isolated from challenger deep sediment (10,898 m) from the mariana trench. Extremophiles, 10, 181–189.Patten C, Glick B R. 2002. Role of Pseudomonas putida in indole acetic acid in development of the host plant root system. Applied and Environmental Microbiology, 68, 3795–3801.Punja Z K. 1985. The biology, ecology, and control of Sclerotium rolfsii. Annual Review of Phytopathology, 23, 97–127.Rakh R R, Raut L S, Dalvi S M, Manwar A V. 2011. Biological control of Sclerotium rolfsii, causing stem rot of groundnut by Pseudomonas cf. monteilii 9. Recent Research in Science and Technology, 3, 26–34.Rimando A M, Duke S O. 2006. Natural Products for Pest Management. ACS Symposium Series No. 927. American Chemical Society. p. 319.Saugar I, Sanz E, Rubio M A, Espinosa J C, Jimenez A. 2002. Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus. European Journal of Biochemistry, 269, 5527–5535.Shew B B, Wynne J C, Beute M K. 1987. Field, microplot, and greenhouse evaluations of resistance to Sclerotium rolfsii in peanut. Plant Disease, 71, 188–191.Shokes F M, Rozalski K, Gorbet D W, Brenneman T B, Berger D A. 1996. Techniques for inoculation of peanut with Sclerotium rolfsii in the greenhouse and field. Peanut Science 23, 124–128.Simi J, Sajjalaguddam R R, Kumar K V K, Varshney R, Sudini H K. 2016. Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii sacc. Journal of General Plant Pathology, 82, 96–104.Simi J, Sudini H K. 2016. Indirect plant growth promotion in grain legumes: Role of actinobacteria. In: Subramaniam G, Sathya A, Vijayabharathi R, eds., Plant Growth Promoting Actinobacteria. Springer, Singapore. pp. 17–32.Singh A, Mehta S, Singh H B, Nautiyal C S. 2003. Biocontrol of collar rot disease of betelvine (Piper betle L.) caused by Sclerotium rolfsii by using rhizosphere-competent Pseudomonas fluorescens NBRI-N6 and P. fluorescens NBRI-N. Current Microbiology, 47, 153–158.Sterner O. 2012. Isolation of microbial natural products. In: Satyajit S D, Lutfun N, eds., Natural Products Isolation. Springer, Humana Press, New York. pp. 393–413.Swadling I, Jeffries P. 1996. Isolation of microbial antagonists for biocontrol of grey mould diseases of strawberries. Biocontrol Science and Technology, 6, 125–136.Taechowisan T, Lu C, Shen Y, Lumyong S. 2005. Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activity. Microbiology, 151, 1691–1695.Tan?i? S, Skrobonja J, Laloševi? M, Jevti? R, Vidi? M. 2013. Impact of Trichoderma spp. on soybean seed germination and potential antagonistic effect on Sclerotinia sclerotiorum. Journal of Pesticides and Phytomedicine (Belgrade), 28, 181–185.Terkina I A, Parfenova V V, Ahn T S. 2006. Antagonistic activity of actinomycetes of Lake Baikal. Applied Biochemistry and Microbiology, 42, 173–176.Vidhyasekaran P, Muthamilan M. 1995. Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Disease, 79, 782–786.Wei G, Kloepper J W, Sadik T. 1991. Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria Phytopathology, 81, 1508–1512.Wokocha C R. 1990. Integrated control of Sclerotium rolfsii infection of tomato in the Nigerian Savanna: Effect of Trichoderma viride and some fungicides. Crop Protection, 9, 231–234.Xiao K, Kinkel L L, Samac D A. 2002. Biological control of phytophthora root rots on alfalfa and soybean with Streptomyces. Biological Control, 23, 285–295. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
