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Journal of Integrative Agriculture  2020, Vol. 19 Issue (3): 700-708    DOI: 10.1016/S2095-3119(19)62640-7
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Detection of seven phytohormones in peanut tissues by ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry
WANG Hai-xia1, 2, WANG Ming-lun2, WANG Xiu-zhong2, DING Yu-long1, 3   
1 College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, P.R.China
2 College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, P.R.China
3 Co-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, P.R.China
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Development of highly sensitive and reliable method for detection of phytohormones is of great significance to study plant hormones and agricultural production.  In this study, an ultra-high-performance liquid chromatography-mass spectrometry/mass spectrometry method was established for separation and quantification of trans-zeatin, trans-zeatin riboside, gibberellin A3, indol-3-acetic acid, salicylic acid, abscisic acid, and jasmonic acid (JA) without any label.  The separation was performed on an Agilent Explus Plus C18 column by using methanol and water as mobile phases with gradient elution.  The target compounds were confirmed and quantified by mass spectrum via positive electrospray ionization for trans-zeatin, trans-zeatin riboside, indole-3-acetic acid, and via negative electrospray ionization for gibberellin3, salicylic acid, abscisic acid, and JA.  The limits of detection ranged from 0.0127 ng L–1 for gibberellin A3 (GA3) to 33.26 ng L–1 for JA and were lower than the currently reported values in literature.  The proposed method was applied for qualitative and quantitative analyses of phytohormones in peanut gynophores and pods.  The recoveries of the spiked phytohormones ranged from 80.20 to 102.56%.  The contents of seven endogenous hormones varied specifically in different development stages of peanuts.  This study provides a highly sensitive and selective detection method for hormones and elucidates the growth and development of the gynophore and peanut fruit, which are controlled by seven endogenous hormones.
Keywords:  phytohormones        simultaneous detection        UHPLC-MS/MS        peanut  
Received: 23 November 2018   Accepted:
Fund: This work was supported by the earmarked fund for China Agriculture Research System (CARS-14) and partly supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (RAPD). We also thank the Natural Science Foundation of Shandong Province, China (ZR2018MB030).
Corresponding Authors:  Correspondence WANG Xiu-zhong, Tel: +86-532-58957442, E-mail:; DING Yu-long, E-mail:   

Cite this article: 

WANG Hai-xia, WANG Ming-lun, WANG Xiu-zhong, DING Yu-long . 2020. Detection of seven phytohormones in peanut tissues by ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry. Journal of Integrative Agriculture, 19(3): 700-708.

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