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Journal of Integrative Agriculture  2016, Vol. 15 Issue (11): 2556-2562    DOI: 10.1016/S2095-3119(16)61382-5
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A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection
ZHAO Xu1, SHAN Ya-nan1, ZHAO Yan1, WANG Ai-rong1, WANG Zong-hua1, 2
1 Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
2 Key Laboratory of Bio-Pesticide and Chemistry Biology, Ministry of Education, Fuzhou 350002, P.R.China
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Abstract        Plant defense responses against penetration or colonization of pathogens are mediated by activation and repression of a large array of genes. Host endogenous small RNAs are essential in gene expression reprogramming process. We identified a new Arabidopsis microRNA (miRNA) ath-miR38-3P by high-throughput sequencing and further confirmed it by Northern blot assay. Interestingly, ath-miR38-3P was highly induced after infection of the pathogen Sclerotinia sclerotiorum. Further analysis based on the miRNA target database demonstrated that ath-miR38-3P might target to five putative genes: AT2G03140, AT5G59430, AT5G66320, AT1G36620 and AT3G03820. To confirm the target, we conducted the quantitative real-time PCR to observe the expression pattern of each candidate gene. The results showed that only AT3G03820 was down-regulated after inoculation of S. sclerotiorum. In addition, overexpression of ath-miR38-3P down-regulates AT3G03820, suggesting AT3G03820 might represent the target for ath-miR38-3P. Our results may provide the useful information for further studying the biological function of a novel ath-miR38-3P and its targets in Arabidopsis-Sclerotinia interaction.
Keywords:  miRNA        Arabidopsis thaliana        Sclerotinia sclerotiorum       miRNA-target gene  
Received: 24 February 2016   Accepted:

This work was supported by grants from the Special Fund for Agro-scientific Research in the Public Interest, China (201103016), and the Fujian Provincial Science Foundation, China (2013J06007).

Corresponding Authors:  WANG Ai-rong, E-mail:; WANG Zong-hua, E-mail:   

Cite this article: 

ZHAO Xu, SHAN Ya-nan, ZHAO Yan, WANG Ai-rong, WANG Zong-hua. 2016. A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection. Journal of Integrative Agriculture, 15(11): 2556-2562.

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