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| Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS |
| DENG Wei, YANG Qian, JIAO Hong-tao, ZHANG Yong-zhi, LI Xue-feng, ZHENG Ming-qi |
| Department of Applied Chemistry, China Agricultural University, Beijing 1000193, P.R.China |
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Abstract Flixweed (Descurainia sophia L.) is a problematic and widespread weed in winter wheat fields and has been controlled by tribenuron-methyl for more than twenty years in China. In this study, a flixweed accession (Hebei 25, HB25) with an Asp-376-Glu mutation in acetohydroxy acid synthase (AHAS) was identified and purified. The purified HB25 accession (pHB25) developed 758.1-fold resistance to tribenuron-methyl and exhibited obvious cross-resistance to four AHAS-inhibiting herbicides. The resistant/susceptible (R/S) ratios of 50% plant growth reduction (GR50) to herbicides of halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 346.1, 15.7, 8.1 and 7.1, respectively. The reduced AHAS sensitivities to four different AHAS-inhibiting herbicides, which were caused by the Asp-376-Glu mutation, were responsible for the resistance and cross-resistance to AHAS-inhibiting herbicides. The R/S ratios of 50% inhibition of AHAS activity (I50) to tribenuron-methyl, halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 844.5, 532.9, 74.5, 13.3 and 5.5, respectively. The results of AHAS activity in vitro were highly correlated with that of whole-plant response experiments.
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Received: 11 March 2016
Accepted:
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| Fund: The project was sponsored by the Special Fund for Agro-scientific Research in the Public Interest, China (201303031). |
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Corresponding Authors:
ZHENG Ming-qi, Tel: +86-10-62734294, E-mail: mqzheng@cau.edu.cn
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Cite this article:
DENG Wei, YANG Qian, JIAO Hong-tao, ZHANG Yong-zhi, LI Xue-feng, ZHENG Ming-qi.
2016.
Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS. Journal of Integrative Agriculture, 15(11): 2563-2570.
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