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Journal of Integrative Agriculture  2022, Vol. 21 Issue (4): 1137-1145    DOI: 10.1016/S2095-3119(21)63766-8
Special Issue: 动物科学合辑Animal Science
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells
ZHU Lu1, 2*, JING Jing1, 2*, QIN Shuai-qi1, 2, LU Jia-ni1, 2, ZHU Cui-yun1, 2, ZHENG Qi1, 2, LIU Ya1, 2, FANG Fu-gui1, 2, LI Yun-sheng1, 2, ZHANG Yun-hai1, 2, LING Ying-hui1, 2
1 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China 
2 Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei 230036, P.R.China
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Abstract  MicroRNA (miRNA) has vital regulatory effects on the proliferation, differentiation and secretion of ovarian granulosa cells, but the role of miR-99a-5p in goat ovarian granulosa cells (GCs) is unclear.  Both miR-99a-5p and Frizzled-5 (FZD5) were found to be expressed in GCs in goat ovaries via fluorescence in situ hybridization and immunohistochemistry, respectively, and FZD5 was verified (P<0.001) as a target gene of miR-99a-5p by double luciferase reporter gene experiments.  Furthermore, FZD5 mRNA and protein expression were both found to be regulated (P<0.05) by miR-99a-5p in GCs.  Moreover, the overexpression of miR-99a-5p or knockdown of FZD5 suppressed (P<0.05) estradiol and progesterone secretion from the GCs, as determined by ELISA.  In summary, miR-99a-5p inhibits target gene FZD5 expression and estradiol and progesterone synthesis in GCs.  Our study thus provides seminal data and new insights into the regulatory mechanisms of follicular development in the goat and other animals.
Keywords:  miRNA       Frizzled-5        follicular development        estradiol        progesterone  
Received: 22 October 2020   Accepted: 04 June 2021
Fund: This work was supported by the National Natural Science Foundation of China (31772566 and 31972629) and the Central Guidance on Local Science and Technology Development Fund of Anhui Province, China (202007d06020005).
About author:  ZHU Lu, E-mail:; JING Jing, E-mail:; Correspondence LING Ying-hui, Tel/Fax: +86-551-65785928, E-mail: * These authors contributed equally to this study.

Cite this article: 

ZHU Lu, JING Jing, QIN Shuai-qi, LU Jia-ni, ZHU Cui-yun, ZHENG Qi, LIU Ya, FANG Fu-gui, LI Yun-sheng, ZHANG Yun-hai, LING Ying-hui. 2022. miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells. Journal of Integrative Agriculture, 21(4): 1137-1145.

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