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Journal of Integrative Agriculture  2022, Vol. 21 Issue (9): 2603-2614    DOI: 10.1016/j.jia.2022.07.024
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Genome-scale mRNA and miRNA transcriptomic insights into the regulatory mechanism of cucumber corolla opening
SONG Xiao-fei1*, GE Dan-feng2*, XIE Yang1*, LI Xiao-li1, SUN Cheng-zhen1, CUI Hao-nan1, ZHU Xue-yun1, LIU Ren-yi2, YAN Li-ying1 #br#

1 Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, P.R.China

2 Center for Agroforestry Mega Data Science, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China 

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为探究黄瓜超级子房花冠开放的分子机制,本研究对超级子房和正常子房花冠发育过程中的mRNA和miRNA进行了测序分析。从4个发育时期共鉴定出234个差异表达miRNAs (DEMs)和291个差异表达靶基因(DE-target genes),其中以黄蕾期的DEMs最多。另外,受5个以上DEMs调控的靶基因有30个,其中CsHD-Zip受28个DEMs调控,其次是DD2X为18个。miRNA_104、miRNA_157、miRNA_349、miRNA_242和miRNA_98在花冠发育过程中表达模式相近,且具有相同的靶基因CsCuRX。此外,通过qRT-PCR进一步对几个关键的候选DEMs及其靶基因进行了验证分析,结果表明miRNA_157-CsCuRX、miRNA_411-CsGH3.6miRNA_161/297/257-CsHD-Zip可能与黄瓜超级子房花冠开放有关


‘Corollas and spines’ is an important trait for fresh market cucumber.  In a unique cucumber line, ‘6457’, the super ovary is much larger and corolla opening is delayed by 4–5 days, thus the resulting fruit has a flower that remains on the tip, which has a high commodity value.  In this study, to better understand the molecular basis of corolla opening, mRNA and miRNA transcriptome analyses were performed during corolla development of the super and normal ovaries.  A total of 234 differentially expressed miRNAs (DEMs) and 291 differentially expressed target genes (DE-target genes) were identified from four developmental stages, and the greatest number of DEMs was found at the yellow bud stage.  Thirty of the DE-target genes were regulated by more than five DEMs, among which, CsHD-Zip was regulated by 28 DEMs, followed by DD2X (18).  In addition, the expression patterns of miRNA_104, miRNA_157, miRNA_349, miRNA_242, and miRNA_98 were similar during corolla development, and they shared the same target gene, CsCuRX.  Moreover, several critical candidate DEMs and DE-target genes were characterized and profiled by a qRT-PCR experiment.  Three of the miRNAs, miRNA_157-CsCuRX, miRNA_411-CsGH3.6, and miRNA_161/297/257-CsHD-Zip, might be responsible for corolla opening in the cucumber super ovary.  This integrated study on the transcriptional and post-transcriptional profiles can provide insights into the molecular regulatory mechanism underlying corolla opening in the cucumber.

Keywords:  cucumber       miRNA       corolla opening       super ovaries  
Received: 04 April 2022   Accepted: 17 February 2022

This work was supported by the National Natural Science Foundation of China (31772327 and 31801877), and the Hebei Vegetable Innovation Projects of Modern Agricultural Industry Technology System, China (HBCT2018030209).  

About author:  Correspondence YAN Li-ying, E–mail:; LIU Ren-yi, E–mail: * These authors contributed equally to this study.

Cite this article: 

SONG Xiao-fei, GE Dan-feng, XIE Yang, LI Xiao-li, SUN Cheng-zhen, CUI Hao-nan, ZHU Xue-yun, LIU Ren-yi, YAN Li-ying. 2022. Genome-scale mRNA and miRNA transcriptomic insights into the regulatory mechanism of cucumber corolla opening. Journal of Integrative Agriculture, 21(9): 2603-2614.

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