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Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage |
XU Yu-chao, HOU Xi-lin, XU Wei-wei, SHEN Lu-lu, LÜ Shan-wu, ZHANG Shi-lin, HU Chun-mei |
1、State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of
Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
2、Agriculture Committee of Feixi County, Hefei 230001, P.R.China |
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摘要 BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What’s more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.
Abstract BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What’s more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.
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Received: 12 January 2015
Accepted:
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Fund: This work was supported by the Independent Innovation Fund for Agricultural Science and Technology of Jiangsu Province, China (CX(15)1015). |
Corresponding Authors:
HU Chun-mei, Tel: +86-25-84395756,E-mail: jjjhcm@njau.edu.cn
E-mail: jjjhcm@njau.edu.cn
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About author: XU Yu-chao, E-mail: 1049205908@qq.com; |
Cite this article:
XU Yu-chao, HOU Xi-lin, XU Wei-wei, SHEN Lu-lu, Lü Shan-wu, ZHANG Shi-lin, HU Chun-mei.
2016.
Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage. Journal of Integrative Agriculture, 15(3): 528-536.
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Champion A, Hebrard E, Parra B, Bournaud C, Marmey P,Tranchant C, Nicole M. 2009. Molecular diversity and geneexpression of cotton ERF transcription factors reveal thatgroup IXa members are responsive to jasmonate, ethyleneand Xanthomonas. Molecular Plant Pathology, 10, 471-485Gutterson N, Reuber T L. 2004. Regulation of diseaseresistance pathways by AP2/ERF transcription factors.Current Opinion in Plant Biology, 7, 465-471Hall B G. 2013. Building phylogenetic trees from moleculardata with MEGA. Molecular Biology and Evolution, 30,1229-1235Hands P, Vosnakis N, Betts D, Irish V F, Drea S. 2011. Alternatetranscripts of a floral developmental regulator have bothdistinct and redundant functions in opium poppy. Annalsof Botany, 107, 1557-1566Huang F, Xu G L, Chi Y J, Liu H C, Xue Q, Zhao T J, Gai J Y,Yu D Y. 2014. A soybean MADS-box protein modulatesfloral organ numbers, petal identity and sterility. BMC PlantBiology, 14, 89.Ito T, Ng K H, Lim T S, Yu H, Meyerowitz E M. 2007. Thehomeotic protein AGAMOUS controls late stamendevelopment by regulating a jasmonate biosynthetic genein Arabidopsis. The Plant Cell, 19, 3516-3529Kizis D, Lumbreras V, Pagès M. 2001. Role of AP2/EREBPtranscription factors in gene regulation during abiotic stress.FEBS Letters, 498, 187-189Li M Y, Wang F, Jiang Q, Ma J, Xiong A S. 2013. Genome-wideanalysis of the distribution of AP2/ERF transcription factorsreveals duplication and elucidates their potential functionin Chinese cabbage (Brassica rapa ssp. pekinensis). PlantMolecular Biology Reporter, 31, 1002-1011McGrath K C, Dombrecht B, Manners J M, Schenk P M,Edgar C I, Maclean D J, Scheible W R, Udvardi M K,Kazan K. 2005. Repressor- and activator-type ethyleneresponse factors functioning in jasmonate signaling anddisease resistance identified via a genome-wide screenof Arabidopsis transcription factor gene expression. PlantPhysiology, 139, 949-959Nakano T, Fujisawa M, Shima Y, Ito Y. 2014. The AP2/ERFtranscription factor SlERF52 functions in flower pedicelabscission in tomato. Journal of Experimental Botany, 65,3111-3119Nakano T, Suzuki K, Fujimura T, Shinshi H. 2006. Genomewideanalysis of the ERF gene family in Arabidopsis andrice. Plant Physiology, 140, 411-432Ohme-Takagi M, Shinshi H. 1995. Ethylene-inducible DNAbinding proteins that interact with an ethylene-responsiveelement. The Plant Cell, 7, 173-182Pak H, Guo Y, Chen M X, Chen K M, Li Y L, Hua S J, ShamsiI, Meng H B, Shi C G, Jiang L X. 2009. The effect ofexogenous methyl jasmonate on the flowering time, floralorgan morphology and transcript levels of a group of genesimplicated in the development of oilseed rape flowers(Brassica napus L.). Planta, 231, 79-91 Pfaffl M W. 2001. A new mathematical model for relativequantification in real-time RT-PCR. Nucleic Acids Research,29, e45.Riechmannn J L, Heard J, Martin G, Reuber L, Jiang C Z, KeddieJ, Adam L, Pineda O, Ratcliffe O J, Samaha R R, CreelmanR, Pilgrim M, Broun P, Zhang J Z, Ghandehari D, Sherman BK, Yu G L. 2000. Arabidopsis transcription factors, genomewidecomparative analysis among eukaryotes. Science,290, 2105-2110Ruttink T, Arend M, Morreel K, Storme V, Rombauts S, FrommJ, Bhalerao R P, Boerjan W, Rohde A. 2007. A moleculartimetable for apical bud formation and dormancy inductionin poplar. The Plant Cell, 19, 2370-2390Sakuma Y, Liu Q, Dubouzet J G, Abe H, Shinozaki K,Yamaguchi-Shinozaki K. 2002. DNA-binding specificity ofthe ERF/AP2 domain of Arabidopsis DREBs, transcriptionfactors involved in dehydration- and cold-induciblegene expression. Biochemical Biophysical ResearchCommunications, 290, 998-1009Sharoni A M, Nuruzzaman M, Satoh K, Shimizu T, KondohH, Sasaya T, Choi I R, Omura T, Kikuchi S. 2011. Genestructures, classification and expression models of theAP2/EREBP transcription factor family in rice. Plant & CellPhysiology, 52, 344-360Tang M J, Sun J W, Liu Y, Chen F, Shen S H. 2006. Isolation andfunctional characterization of the JcERF gene, a putativeAP2/EREBP domain-containing transcription factor, in thewoody oil plant Jatropha curcas. Plant Molecular Biology,63, 419-428Yellina A L, Orashakova S, Lange S, Erdmann R, Leebens-Mack J, Becker A. 2010. Floral homeotic C function genesrepress specific B function genes in the carpel whorl of thebasal eudicot California poppy (Eschscholzia californica).EvoDevo, 1, 13.Zhang L X, Li Z F, Quan R D, Li G J, Wang R G, Huang R F.2011. An AP2 domain-containing gene, ESE1, targetedby the ethylene signaling component EIN3 Is important forthe salt response in Arabidopsis. Plant Physiology, 157,854-865Zhang Z J, Wang J, Zhang R X, Huang R F. 2012. Theethylene response factor AtERF98 enhances tolerance tosalt through the transcriptional activation of ascorbic acidsynthesis in Arabidopsis. The Plant Journal, 71, 273-287Zhao T J, Sun S, Liu Y, Liu J M, Liu Q, Yan Y B, Zhou HM. 2006. Regulating the drought-responsive element(DRE)-mediated signaling pathway by synergic functionsof trans-active and trans-inactive DRE binding factors inBrassica napus. The Journal of Biological Chemistry, 281,10752-10759Zhuang J, Cai B, Peng R H, Zhu B, Jin X F, Xue Y, Gao F, Fu XY, Tian Y S, Zhao W, Qiao Y S, Zhang Z, Xiong A S, Yao QH. 2008. Genome-wide analysis of the AP2/ERF gene familyin Populus trichocarpa. Biochemical Biophysical ResearchCommunications, 371, 468-474Zhuang J, Peng R H, Cheng Z M, Zhang J, Cai B, Zhang Z,Gao F, Zhu B, Fu X Y, Chen J M, Qiao Y S, Xiong A S,Yao Q H. 2009. Genome-wide analysis of the putative AP2/ERF family genes in Vitis vinifera. Scientia Horticulturae,123, 73-81 |
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