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Journal of Integrative Agriculture  2016, Vol. 15 Issue (3): 528-536    DOI: 10.1016/S2095-3119(15)61203-5
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage
 XU Yu-chao, HOU Xi-lin, XU Wei-wei, SHEN Lu-lu, LÜ Shan-wu, ZHANG Shi-lin, HU Chun-mei
1、State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of
Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
2、Agriculture Committee of Feixi County, Hefei 230001, P.R.China
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摘要  BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What’s more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.

Abstract  BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What’s more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.
Keywords:  non-heading Chinese cabbage       stamen-petalody       ethylene-responsive factor       gene expression  
Received: 12 January 2015   Accepted:
Fund: 

This work was supported by the Independent Innovation Fund for Agricultural Science and Technology of Jiangsu Province, China (CX(15)1015).

Corresponding Authors:  HU Chun-mei, Tel: +86-25-84395756,E-mail: jjjhcm@njau.edu.cn     E-mail:  jjjhcm@njau.edu.cn
About author:  XU Yu-chao, E-mail: 1049205908@qq.com;

Cite this article: 

XU Yu-chao, HOU Xi-lin, XU Wei-wei, SHEN Lu-lu, Lü Shan-wu, ZHANG Shi-lin, HU Chun-mei. 2016. Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage. Journal of Integrative Agriculture, 15(3): 528-536.

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