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Special Issue:
动物科学合辑Animal Science
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| Dietary threonine deficiency affects expression of genes involved in lipid metabolism in adipose tissues of Pekin ducks in a genotype-dependent manner |
| JIANG Yong1*, MA Xin-yan3*, XIE Ming2, ZHOU Zheng-kui2, TANG Jing2, CHANG Guo-bin1, CHEN Guo-hong1, HOU Shui-sheng2 |
1 College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China
2 Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs/State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 State Key Laboratory of Livestock and Poultry Breeding/Key Laboratory of Animal Nutrition and Feed Science in South China/Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China
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摘要
本试验通过高通量测序技术测定脂肪型和瘦肉型北京鸭肝脏、皮脂和腹脂基因表达谱，以比较脂肪型和瘦肉型北京鸭脂质代谢差异表达基因。分别饲喂脂肪型和瘦肉型北京鸭苏氨酸缺乏或者充足的饲粮21天（15-35日龄）。试验结果表明，饲粮苏氨酸缺乏分别影响瘦肉型北京鸭肝脏、皮脂和腹脂中187、52和50个基因表达，其中分别有12、9和5个基因与脂质代谢有关。饲粮苏氨酸缺乏分别影响脂肪型北京鸭肝脏、皮脂和腹脂中27、6和3个基因表达，未发现与脂质代谢相关基因。KEGG分析表明瘦肉型北京鸭肝脏差异表达基因主要富集在脂质代谢通路（油酸代谢、甘油磷脂代谢和花生四烯酸代谢）和氨基酸代谢通路中（氨基酸合成、苯丙氨酸代谢、β-丙氨酸代谢、甘氨酸-丝氨酸-苏氨酸代谢）。脂肪型北京鸭脂肪组织中差异表达基因没有富集到脂质和氨基酸代谢通路中。苏氨酸缺乏增加瘦肉型北京鸭肝脏中苹果酸脱氢酶3、酰基辅酶a合成酶短链家族成员2和皮脂中脂肪酶M的表达。综上所述，饲粮苏氨酸缺乏对北京鸭脂质组织脂肪代谢相关基因表达的影响与北京鸭基因型有关。该研究发现表明北京鸭的遗传选育改变了其基因背景，为充分理解苏氨酸对不同品系北京鸭脂质代谢作用提供了一个新的视角，也为苏氨酸在北京鸭饲粮中合理添加提供了理论基础。
Abstract Dietary threonine (Thr) deficiency increases hepatic triglyceride content and reduces sebum and abdominal fat percentages in lean type (LT), but not in fatty type (FT) Pekin ducks. However, the molecular changes regarding the role of Thr in lipid metabolism in LT and FT ducks induced by Thr deficiency remains unknown. This study compared differential expression gene profiles related to lipid metabolism in FT and LT Pekin ducks affected by Thr deficiency. We performed transcriptomic profiling and scanned the gene expression in the liver, sebum, and abdominal fat of Pekin ducks fed either Thr-deficient or Thr-adequate diet for 21 days from 14 to 35 days of age. There were 187, 52, and 50 differentially expressed genes (DEGs) identified in the liver, sebum, and abdominal fat of LT ducks affected by Thr deficiency, of which 12, 9, and 5 genes were involved in lipid metabolism, respectively. Thr deficiency altered the expression of 27, 6, and 3 genes in FT ducks’ liver, sebum, and abdominal fat, respectively. None of the DEGs had a relationship with lipid metabolism in FT ducks. KEGG analysis showed that the DEGs in the LT ducks’ livers were enriched in lipid metabolism pathways (linolenic acid metabolism, glycerophospholipid metabolism, and arachidonic acid metabolism) and amino acid metabolism pathways (biosynthesis of amino acids, phenylalanine metabolism, β-alanine metabolism, and glycine, serine and threonine metabolisms). The DEGs in the sebum and abdominal fat of LT ducks were not enriched in lipid and amino acid metabolic pathways. Additionally, DEGs involved in lipid metabolism were found to be upregulated by Thr deficiency in LT ducks, such as malic enzyme 3 (ME3), acyl-CoA synthetase short-chain family member 2 (ACSS2) in liver, and lipase member M (LIPM) in sebum. In summary, dietary Thr deficiency regulated the gene expression involved in lipid metabolism in the liver, sebum, and abdominal fat of Pekin ducks in a genotype-dependent manner.
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Received: 24 March 2021
Accepted: 27 August 2021
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| Fund: This work was supported by the National Natural Science Foundation of China (31902174), the Natural Science Foundation of Jiangsu Province, China (BK20190902), and the Natural Science Foundation of Jiangsu Higher Education Institutions of China (19KJD230003). |
| About author: JIANG Yong, E-mail: jiangyong12126@163.com; MA Xin-yan, E-mail: dk051maxy@163.com; Correspondence CHEN Guo-hong, E-mail: ghchen@yzu.edu.cn; HOU Shui-sheng, E-mail: houss@263.net
* These authors contributed equally to this study. |
Cite this article:
JIANG Yong, MA Xin-yan, XIE Ming, ZHOU Zheng-kui, TANG Jing, CHANG Guo-bin, CHEN Guo-hong, HOU Shui-sheng.
2022.
Dietary threonine deficiency affects expression of genes involved in lipid metabolism in adipose tissues of Pekin ducks in a genotype-dependent manner. Journal of Integrative Agriculture, 21(9): 2691-2699.
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