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Effects of potassium deficiency on photosynthesis and photoprotection mechanisms in soybean (Glycine max (L.) Merr.) |
WANG Xiao-guang, ZHAO Xin-hua, JIANG Chun-ji, LI Chun-hong, CONG Shan, WU Di, CHEN Yan-qiu, YU Hai-qiu, WANG Chun-yan |
1、College of Agronomy, Shenyang Agricultural University, Shenyang 110866, P.R.China
2、Institute of Crop Research, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R.China
3、Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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摘要 Potassium is an important nutrient element requiring high concentration for photosynthetic metabolism. The potassium deficiency in soil could inhibit soybean (Glycine max (L.) Merr.) photosynthesis and result in yield reduction. Research on the photosynthetic variations of the different tolerant soyben varieties should provide important information for high yield tolerant soybean breeding program. Two representative soybean varieties Tiefeng 40 (tolerance to K+ deficiency) and GD8521 (sensitive to K+ deficiency) were hydroponically grown to measure the photosynthesis, chlorophyll fluorescence parameters and Rubisco activity under different potassium conditions. With the K-deficiency stress time extending, the net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) of GD8521 were significantly decreased under K-deficiency condition, whereas the intercellular CO2 concentration (Ci) was significantly increased. As a contrast, the variations of Tiefeng 40 were almost little under K-deficiency condition, which indicated tolerance to K+ deficiency variety could maintain higher efficient photosynthesis. On the 25th d after treatment, the minimal fluorescence (F0) of GD8521 was significantly increased and the maximal fluorescence (Fm), the maximum quantum efficiency of PSII photochemistry (Fv/ Fm), actual photochemical efficiency of PSII (ΦPSII), photochemical quenching (qP), and electron transport rate of PSII (ETR) were significantly decreased under K+ deficiency condition. In addition, the Rubisco content of GD8521 was significantly decreased in leaves. It is particularly noteworthy that the chlorophyll fluorescence parameters and Rubisco content of Tiefeng 40 were unaffected under K+ deficiency condition. On the other hand, the non-photochemical quenching (qN) of Tiefeng 40 was significantly increased. The dry matter weight of Tiefeng 40 was little affected under K+ deficiency condition. Results indicated that Tiefeng 40 could avoid or relieve the destruction of PSII caused by exceeded absorbed solar energy under K-deficiency condition and maintain natural photosynthesis and plant growth. It was an essential physiological mechanism for low-K-tolerant soybean under K-deficiency stress.
Abstract Potassium is an important nutrient element requiring high concentration for photosynthetic metabolism. The potassium deficiency in soil could inhibit soybean (Glycine max (L.) Merr.) photosynthesis and result in yield reduction. Research on the photosynthetic variations of the different tolerant soyben varieties should provide important information for high yield tolerant soybean breeding program. Two representative soybean varieties Tiefeng 40 (tolerance to K+ deficiency) and GD8521 (sensitive to K+ deficiency) were hydroponically grown to measure the photosynthesis, chlorophyll fluorescence parameters and Rubisco activity under different potassium conditions. With the K-deficiency stress time extending, the net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) of GD8521 were significantly decreased under K-deficiency condition, whereas the intercellular CO2 concentration (Ci) was significantly increased. As a contrast, the variations of Tiefeng 40 were almost little under K-deficiency condition, which indicated tolerance to K+ deficiency variety could maintain higher efficient photosynthesis. On the 25th d after treatment, the minimal fluorescence (F0) of GD8521 was significantly increased and the maximal fluorescence (Fm), the maximum quantum efficiency of PSII photochemistry (Fv/ Fm), actual photochemical efficiency of PSII (ΦPSII), photochemical quenching (qP), and electron transport rate of PSII (ETR) were significantly decreased under K+ deficiency condition. In addition, the Rubisco content of GD8521 was significantly decreased in leaves. It is particularly noteworthy that the chlorophyll fluorescence parameters and Rubisco content of Tiefeng 40 were unaffected under K+ deficiency condition. On the other hand, the non-photochemical quenching (qN) of Tiefeng 40 was significantly increased. The dry matter weight of Tiefeng 40 was little affected under K+ deficiency condition. Results indicated that Tiefeng 40 could avoid or relieve the destruction of PSII caused by exceeded absorbed solar energy under K-deficiency condition and maintain natural photosynthesis and plant growth. It was an essential physiological mechanism for low-K-tolerant soybean under K-deficiency stress.
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Received: 31 March 2014
Accepted:
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Fund: This work were supported by the National Natural Science Foundation of China (31271644), the Program for Liaoning Excellent Talents in University (LNET), China and the Tianzhu Mountian Scholars Support Plan of Shenyang Agricultural University, China. |
Corresponding Authors:
YU Hai-qiu,Tel/Fax: +86-24-88487135, E-mail: haiqiuyu@163.com;WANG Chun-yan, Tel/Fax: +86-10-82106721,E-mail: wangchunyan@caas.cn
E-mail: haiqiuyu@163.com;wangchunyan@caas.cn
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About author: WANG Xiao-guang, E-mail: wxglyj@163com; ZHAO Xin-hua,E-mail: zxh0427@126.com;* These authors contributed equally to this study |
Cite this article:
WANG Xiao-guang, ZHAO Xin-hua, JIANG Chun-ji, LI Chun-hong, CONG Shan, WU Di, CHEN Yan-qiu, YU Hai-qiu, WANG Chun-yan.
2015.
Effects of potassium deficiency on photosynthesis and photoprotection mechanisms in soybean (Glycine max (L.) Merr.). Journal of Integrative Agriculture, 14(5): 856-863.
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Abbasi M K, Tahir M M, Azam W, Abbs Z, Rahim N. 2012.Soybean yield and chemical composition in response tophosphorus-potassium nutrition in Kashmir. AgronomyJournal, 104, 1476-1484Agata R, Mario R, Linda M. 2001. Enhanced osmotolerance ofa wheat mutant selected for potassium accumulation. PlantScience, 160, 441-448Ao X, Guo X H, Zhu Q, Zhang H J, Wang H Y, Ma Z H, Han X R,Zhao M H , Xie F T. 2014. Effect of phosphorus fertilizationto P uptake and dry matter accumulation in soybean withdifferent P efficiencies. Journal of Integrative Agriculture,13, 326-334Bednarz C W, Oosterhuis D M, Evans R D. 1998. Leafphotosynthesis and carbon isotope discrimination of cottonin response to potassium deficiency. Environmental andExperimental Botany, 39, 131-139Bednarz C W, Oosterhuis D M. 1999. Physiological changesassociated with potassium deficiency in cotton. Journal ofPlant Nutrition, 22, 303-313Bilger W, Björkman O. 1990. Role of the xanthophyllcycle in photoprotection elucidated by measurementsof light-induced absorbance changes, fluorescenceand photosynthesis in leaves of Hedera canariensis.Photosynthesis Research, 25, 173-185Briantais J M, Comic G, Hodges M. 1998. The modificationof chlorophyll fluorescence of Chamydomonas reinhardtiiby photoinhibition and chloramphenicol addition suggestsa form of photosystem II less susceptible to degradation.FEBS Letters, 236, 226-230Broadley M R, White P J. 2005. Plant Nutritional Genomics.Blackwell Publishing Ltd., Oxford, UK. pp. 22-65Cakmak I. 2005. The role of potassium in alleviating detrimentaleffects of abiotic stresses in plants. Journal of Plant Nutritionand Soil Science, 168, 521-530Cao M J, Yu H Q, Yan H K, Jiang C J. 2007. difference intolerance to potassium deficiency between two maize inbredlines. Plant Production Science, 10, 42-46Chen J J, Warren H G. 2000. Morphological and physiologicalcharacteristics of tomato roots associated with potassiumacquisitionefficiency. Horticultural Science, 83, 213-225Coskun D, Britto D T, Kronzucker H J. 2014. The physiology ofchannel-mediated K+ acquisition in roots of higher plants.Physiologia Plantarum, 151, 305-312Dannehl H, Wietoska H, Heckmann H, Godde D. 1996. Changesin D1 protein turnover and recovery of photo system IIactivity precede accumulation of chlorophyll in plants afterrelease from mineral stress. Planta, 199, 34-42Degl’Innocenti E, Hafsi C, Guidi L, Navari-Izzo F. 2009. Theeffect of salinity on photosynthetic activity in potassiumdeficientbarley species. Jourmal of Plant Physiology, 166,1968-1981Demmig-Adams B, Adams W W, Heber U, Neimanis S, WinterK, Kruger A, Czygan F C, Bilger W, Bjorkman O. 1990.Inhibition of zeaxanthin formation and of rapid changes inradiationless energy dissipation by dithiothreitol in spinachand chloroplasts. Plant Physiology, 92, 293-301Hermans C, Hammond J P, White P J, Verbruggen N. 2006.How do plants respond to nutrient shortage by biomassallocation? Trends Plant Science, 11, 610-617Jiang C C, Chen F, Gao X Z, Lu J W, Wan K Y, Nian F Z, WangY H. 2008. Study on the nutrition characteristics of differentK use efficiency cotton genotypes to K deficiency stress.Agricultural Science in China, 7, 740-745Jiang D A, Lu Q, Xue J M, Xie X M. 1992. Regulation ofpotassium nutrition to photosynthetic function and lightenergyabsorption of rice leaf. Acta Agriculturae UniversitatisZhejiangensis, 18, 25-29 (in Chinese)Jiang D A, Lu Q, Weng X Y, Zhen B S, Xi H F. 2000. Regulationof Rubisco carboxylation activity and photosynthetic rate byRubisco activase during leaf senescence in rice. Journalof Zhejiang University (Agriculture & Life Sciences), 26,119-124 (in Chinese)Jin J Y. 2012. Changes in the efficiency of fertiliser use inChina. Journal of the Science of Food and Agriculture, 92,1006-1009Kanai S, Ohkura K, Adu-Gyamfi J J, Mohapatra P K, NguyenN T, Saneoka H, Fujita K. 2007. Depression of sink activityprecedes the inhibition of biomass production in tomatoplant subjected to potassium deficiency stress. Journal ofExperimental Botany, 58, 2917-2928Lazár D. 1999. Chlorophyll a fluorescence induction. Biochimicaet Biophysica Acta-Bioenergetics, 1412, 1-28Li C H, Sun H Y, Sun J, Li X T, Du Y X, Cao M J. 2011. Differenceof tolerance to low potassium in soybean varieties (lines).Journal of Shenyang Agricultrual University, 42, 649-653(in Chinese)Li X Y, Mu C S, Lin J X, Wang Y, Li X J. 2014. Effect of alkalinepotassium and sodium salts on growth, photosynthesis,ions absorption and solutes synthesis of wheat seedlings.Experimental Agriculture, 50, 144-157Lichtenthaler H K, Babani F. 2004. Light adaptation andsenescence of the photosynthetic apparatus. Changesin pigment composition chlorophyll fluorescenceparameters and photosynthetic activity. In: PapageoriouG, Govindjee, eds., Chlorophyll Fluorescence: A Signatureof Photosynthesis. Springer, Dordrecht, The Netherlands.pp. 713-736Lichtenthaler H K. 1996. Vegetation stress: an introduction to the stress concept in plants. Journal of Plant Physiology,148, 4-14Marschner H, Marschner P. 2012. Marschner’s Mineral Nutritionof Higher Plants. Elsevier, London, UK.Maxwell K, Johnson G N. 2000. Chlorophyll fluorescence - Apractical guide. Journal of Experimental Botany, 51,659-668Mäser P, Gierth M, Schroeder J I. 2002. Molecular mechanismsof potassium and sodium uptake in plants. Plant and Soil,247, 43-54Mengel K, Kirkby E A. 2001. Principles of Plant Nutrition. KluwerAcademic Publishers, Dordrecht, The Netherlands. p. 833.Müller P, Li X P, Niyogi K K. 2001. Non-photochemicalquenching: a response to excess light energy. PlantPhysiology, 125, 1558-1566Peasles D E, Moss D N. 1966. Photosynthesis in K- and Mgdeficientmaize leaves. Soil Science, 30, 220-223Pervez H, Ashraf M, Makhdum M I. 2004. Influence ofpotassium nutrition on gas exchange characteristicsand water relations in cotton (Gossypium hirsutum L.).Photosynthetica, 42, 251-255Pettigrew W T. 2008. Potassium influences on yield andquality production for maize, wheat, soybean and cotton.Physiologia Plantarum, 133, 670-681Qu C X, Liu C, Gong X L, Li C X, Hong M M, Wang L, Hong F S.2012. Impairment of maize seedling photosynthesis causedby a combination of potassium deficiency and salt stress.Environmental and Experimental Botany, 75, 134-141Römheld V, Kirkby E. 2010. Research on potassium inagriculture: needs and prospects. Plant Soil, 335, 155-180Schnettger B, Critchley C, Santore U J. 1994. Relationshipbetween photo inhibition of photosynthesis, D1 proteinturnover and chloroplast structure: Effect of proteinsynthesis. Plant Cell Environment, 17, 55-64Schreiber U, Bilger W, Neubauer C. 1995. Chlorophyllfluorescence as a nonintrusive indicator for rapid assessmentof in vivo photosynthesis. In: Schulze E D, Caldwell M M,eds., Ecophysiology of Photosynthesis. Springer-Verlag,Berlin. pp. 49-70Sharkey T D, Savitch L V, Butz N D. 1991. Photometric methodfor routine determination of Kcat and carbamylation ofRubisco. Photosynth Research, 28, 41-48Sun J, Li C H, Sun H Y, Cao M J, Wang X G. 2011. The effectof low potassium stress on growth and development ofdifferent soybean genotypes. Chinese Journal of SoilScience, 42, 431-434 (in Chinese)Wang X L, Yu H Q, Liu N, Yi B, Cao M J. 2012. Physiologicalcharacteristics of delaying leaf senescence in maize inbredlines tolerant to potassium deficiency. Acta AgronomicaSinica, 38, 1672-1679 (in Chinese)Wang Y, Wu W H. 2013. Potassium transport and signaling inhigher plants. Annual Review of Plant Biology, 64, 451-476Yin X, Vyn T J. 2004. Critical leaf potassium concentrationsfor yield and seed quality of conservation-till soybean. SoilScience Society of America Joutnal, 68, 1626-1634Zhao D, Oosterhuis D M, Bednarz C W. 2001. Influenceof potassium deficiency on photosynthesis, chlorophyllcontent, and chloroplast ultrastructure of cotton plants.Photosynthetica, 39, 103-109 |
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