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Special Issue:
线虫合辑Nematology
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| Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae |
| LIU Yan-ke, HUANG Wen-kun, LONG Hai-bo, PENG Huan, HE Wen-ting , PENG De-liang |
| State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China |
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摘要 For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene (Ha-acp1) expressed in the subventral glands of the cereal cyst nematode (Heterodera avenae) was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated specifically in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.
Abstract For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene (Ha-acp1) expressed in the subventral glands of the cereal cyst nematode (Heterodera avenae) was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated specifically in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.
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Received: 23 April 2012
Accepted:
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| Fund: the fund of the National Basic Research Program of China (2013CB127502), the Special Fund for Agro-Scientific Research in the Public Interest, China (200903040) and the National Natural Science Foundation of China (31201493). |
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Corresponding Authors:
PENG De-liang, E-mail: dlpeng@ippcaas.cn
E-mail: dlpeng@ippcaas.cn
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| About author: LIU Yan-ke, E-mail: Shmilyake@126.com |
Cite this article:
LIU Yan-ke, HUANG Wen-kun, LONG Hai-bo, PENG Huan, HE Wen-ting , PENG De-liang.
2014.
Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae. Journal of Integrative Agriculture, 13(6): 1303-1310.
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