【Objective】This study was conducted to investigate the effects of mannan oligosaccharides (MOS) supplementation to milk replacer on the development of gastrointestinal tract of 7-28 day-old Hu lambs. 【Method】 Thirty 7 day-old Hu male lambs were chosen and divided into 2 groups randomly, fifteen lambs in each group and each lamb as a repeat. Lambs were fed milk replacer with or without 0.2 % MOS, respectively. The test lasted for 21 days. Eight lambs were selected from each group randomly and slaughtered at 28 day-old. The weights of the compound stomach and the intestinal tract with and without content, and lengths of the intestinal tract were measured, and the relative quality and length were calculated. While the tissue samples from fundus gland region of the abomasum, the middle part of duodenum, jejunum and ileum were fixed in paraformaldehyde to analyse the histomorphology, and the apoptotic rate of intestinal epithelial cells as well. And the mRNA expression of claudin 1, zonula occludens-1 (ZO-1), and occludin protein of duodenum, jejunum and ileum mucosa were measured.【Result】The results showed that except relative length of jejunum (P=0.040), the relative weights (% body weight, % stomach weight, % intestinal tract weight, and % gastrointestinal tract weight), relative lengths (% intestinal tract length), content of stomach and intestinal tract (% body weight, % stomach content weight, % intestinal tract content weight, and % gastrointestinal tract content weight), the apoptotic rate of intestinal epithelial cells and mRNA expression of claudin 1 protein in intestinal tract of lambs were not affected by MOS (P>0.05). However, MOS elongated the villus height and the muscular thickness, and decreased the villus width of lamb duodenum significantly (P=0.033, P=0.047, P=0.015). MOS also up-regulated the mRNA expression of ZO-1 protein of lamb jejunum significantly (P=0.028). And there was a tendency that MOS elongated villus height, width and crypt depth of ileum, muscular thickness of abomasum, and mRNA expression of occludin protein of ileum (P=0.075, P=0.078, P=0.085, P=0.084, P=0.052).【Conclusion】MOS almost did not affect the relative weights, lengths, and content distribution of gastrointestinal tract of 7-28 days old Hu lambs, but improved the histomorphology of duodenum and ileum, indicating it could maintain barrier function of intestinal tract and benefit to nutrients digestibility.