Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (1): 169-175 .doi: 10.3864/j.issn.0578-1752.2008.01.022

• HORTICULTURE • Previous Articles     Next Articles

Cloning and Analysis of Resistance Gene Analogs from Pepper (Capsium annuum L.)

  

  1. 华中农业大学作物遗传改良国家重点实验室
  • Received:2006-10-30 Revised:2007-02-06 Online:2008-01-10 Published:2008-01-10

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Abstract: 【Objective】 Resistance gene analogs are isolated from pepper using homology-based method. 【Method】The genomic DNA of pepper (Capsium annuum L.) line PR205 was amplified using degenerated primers which were designed based on the conserved regions of Nucleotide Binding Site (NBS)-Leucine Rich Repeat (LRR) domain from previously reported plant resistance genes. 【Result】Twenty-five Resistance Gene Analogs (RGAs) have been obtained. These RGAs were classified into seven distinct groups by phylogenetic analysis. Putative amino acid sequences deduced from these 25 RGAs shared 27.4%-98.2% identity with the resistance genes of Mi-1.2, prf, Hero, I2C-1 and RPM1. RGA-p20 showed an extreme sequence identity (99%) with Mi-1.2 at nucleotide/amino acid levels. It suggests that there is a root knot nematode resistance gene which is homology with Mi gene in pepper of PR205 and RGA-p20 is one part of this resistance gene.【Conclusion】In this study, twenty-five RGAs were obtained in pepper line PR205, which laid a promsing foundation for cloning of resistance genes from pepper.

Key words: pepper, RGAs, resistance gene, NBS-LRR

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