戴尔有孢圆酵母与酿酒酵母细胞间接触对‘赤霞珠’葡萄酒风味品质的影响

doi:10.3864/j.issn.0578-1752.2024.16.013

Abstract

Abstract:

【Objective】The mixed fermentation of Saccharomyces cerevisiae and Non-Saccharomyces yeast could improve the complexity and richness of wine aroma. In order to accurately control the mixed fermentation, it is necessary to deeply elucidate the regulatory effects of the contact between yeast cells in the co-culture on alcohol fermentation and metabolites.【Method】Torulaspora delbrueckii (T. delbrueckii) and S. cerevisiae was inoculated in the sterilized Cabernet Sauvignon grape juice to conduct pure fermentation, mixed fermentation, and double-compartment fermentation, respectively, and the differences in fermentation kinetics and volatile aroma compounds were analyzed. In addition, the effects of mixed fermentation and double-compartment fermentation on the vinification parameters and flavor quality of Cabernet Sauvignon wine under different inoculation time modes were investigated.【Result】T. delbrueckii strain was unable to complete the alcoholic fermentation independently, resulting in a final reducing sugar content of 89.00 g∙L^-1 in its fermented wine. S. cerevisiae maintained high growth activity in pure fermentation, mixed fermentation as well as double-compartment fermentation, and successfully completed alcohol fermentation. Cell-to-cell contact during mixed fermentation significantly reduced the viability of T. delbrueckii. Compared with the pure fermentation of S. cerevisiae, the mixed and the double-compartment fermentation were characterized by high content of the total acid and low level of ethanol and pH value. The content of volatile acid detected in each treated wine sample was between 0.2-0.7 g∙L^-1. The content of volatile acid and anthocyanin in the mixed fermentation group was significantly lower than those in S. cerevisiae pure fermentation and double-compartment fermentation. The results of GC-MS showed that the content of aroma compounds in T. delbrueckii strain pure fermentation group was the lowest among all wine samples. Compared with the S. cerevisiae pure fermentation, the content of esters in the mixed fermentation and the double-compartment fermentation significantly increased, while the content of higher alcohols and C₆ compounds significantly decreased. The content of ester compounds, such as isoamyl acetate, hexyl acetate, ethyl caproate and ethyl caprylate in the mixed fermentation group, showed a significant increase trend compared with those in the double-compartment fermentation group. Meanwhile, there was a significant decrease in the levels of higher alcohols and benzene derivatives. In addition, the inoculation time of S. cerevisiae also had a significant effect on the formation of isoamyl acetate and hexyl acetate during mixed fermentation. The results of sensory analysis showed that the simultaneous inoculation (0 h) strategy could significantly reduce the green flavor and enhance the fruity and floral aroma of wine samples compared with S. cerevisiae pure fermentation.【Conclusion】During the mixed alcoholic fermentation, the yeast cell-to-cell contact not only limited the growth of T. delbrueckii, but also significantly affected the aroma characteristics and sensory quality of Cabernet Sauvignon wine.

Key words: wine, Alcoholic fermentation, Non-Saccharomyces yeasts, Saccharomyces cerevisiae, cell-to-cell contact, volatile compounds, flavor quality

ZHOU DeGang, XU BinYan, WANG QingXia, ZHU Xia, YANG XueShan. Effects of Cell-to-Cell Contact Between Torulaspora delbrueckii and Saccharomyces cerevisiae on the Flavor and Quality of Cabernet Sauvignon Wine[J].Scientia Agricultura Sinica, 2024, 57(16): 3264-3282.

Figures/Tables 12

Fig. 1

Table 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Table 2

The content of volatile compounds with OAV>0.1 in Cabernet Sauvignon wine fermented with different strategies"

化合物 Compound	阈值 Threshold (μg∙L^-1)	气味活性值 Odor activity value		香气物质质量浓度Aroma concentration (g∙L^-1)										气味描述 Odor description
化合物 Compound	阈值 Threshold (μg∙L^-1)	气味活性值 Odor activity value		P-Td		P-Sc		M_0h-Td/Sc		M_48h-Td/Sc		D_0h-Td/Sc	D_48h-Td/Sc	气味描述 Odor description
乙酸酯Acetate ester
乙酸异戊酯 Isoamyl acetate	30^[30]	>1.0		240.67±11.63f		1841.62±49.65e		2379.82±16.75b		2056.2±17.14c		2622.37±32.56a	1924.82±22.58d	苹果、香蕉、水果 Apples, bananas, fruit
乙酸己酯 Hexyl acetate	670^[30]	>0.1		9.87±2.21d		235.91±44.94b		295.98±2.06a		273.99±11.62a		284.78±6.77a	130.12±6.8c	苹果、香蕉、草本 Apples, bananas, herbs
乙酸辛酯 Octyl acetate	50^[29]	>0.1		/		10.21±0.19a		/		9.22±1.53b		6.7±1.02c	/	柑橘、脂肪、木材 Citrus, fat, wood
脂肪酸乙酯Ethyl ester
己酸乙酯 Ethyl hexanoate	14^[30]	>1.0		80.58±11.86f		563.19±24.7e		867.89±14.19b		685.47±16.9d		950.76±11.91a	798.21±13.59c	茴香、香蕉、白兰地 Fennel, banana, brandy
辛酸乙酯 Ethyl caprylate	5^[30]	>1.0		125.37±13.6d		3068.89±79.64c		5322.07±511.95a		3213.94±180.79c		3913.51±3.49b	4050.86±212.1b	杏、香蕉、白兰地 Apricots, bananas, brandy
癸酸乙酯 Ethyl caprate	200^[30]	>1.0		461±4.9e		1176.4±128.15d		1550.75±2.27b		1311.93±100.42c		1943±26.81a	531.58±2.93e	白兰地、天竺葵 Brandy, geranium
其他酯Other esters
辛酸甲酯 Methyl octylate	200^[29]	>0.1		/		25.56±1.93a		10.87±1.59b		/		/	/	水果、橙子、甜味 Fruit, orange, sweet
丙酸己酯 Hexyl propionate	8^[30]	>1.0		/		31.27±0.3a		17.65±0.68b		/		10.73±0.73c	/	*
正辛酸异戊酯 Isoamyl octylate	125^[30]	>0.1		/		13.93±3.36b		20.63±4.99a		/		7.79±0.03c	/	烤苹果 Roast apple
高级醇Higher alcohol
异戊醇 Isoamylol	30000^[30]	>0.1		2900.7±225.8e		4431.76±136.36a		3753.93±290.09c		3470.63±18.08d		4040.31±126.07b	3807.05±291.5bc	苦杏仁、香蕉 Bitter almonds, bananas
正庚醇 Heptan-1-ol	200^[30]	>0.1		6.1±0.63d		32.68±2.27a		9.25±0.01c		25.3±1b		4.08±0.44e	7.03±0.23d	柠檬、柑橘 Lemon, citrus,
正辛醇 1-Octanol	40^[31]	>0.1		/		40.15±1.71a		/		30.62±3.31b		/	/	柑橘、玫瑰、茉莉 Citrus, rose, jasmine
苯衍生物Benzene derivative
乙酸苯乙酯 Phenethyl acetate	250^[31]	>0.1		223.55±7.43d		635.44±11.41c		748.95±28.74b		616.93±41.09c		874.39±69.95a	625.54±14.26c	玫瑰、花香、果香 Rose, floral, fruity aromas
苯乙醇 Phenethyl alcohol	10000^[31]	>0.1		2570.47±399.9b		2604.23±88.12b		3101.56±141.2a		2495.06±257.03b		3129.64±209.56a	2632.84±163.54b	玫瑰、花香、蜂蜜 Rose, floral fragrance, honey
脂肪酸Fatty acids
2-甲基丁酸 2-Methylbutyric acid	30^[29]	>0.1		73.71±0.34a		29.38±1.19c		19.63±1.29d		35.64±4.26b		30.46±3.74c	21.57±1.53d	辛辣、乳酪 Spicy, cheese
辛酸 Octanoic acid	500^[32]	>0.1		24.3±5.7c		103.4±30.3a		86.42±2.36ab		33.94±0.9c		73.2±13.84b	20.73±4.81c	奶酪、脂肪 Cheese, fat
萜烯类Terpene
芳樟醇 Linalool	15^[31]	>0.1	18.78±0.62a		12.27±0.46d		13.87±0.93c		12.04±0.09d		17.11±0.69b		19.35±0.35a	花香、果香、葡萄 Floral, fruity, grape
乙酸香叶酯 Geranyl acetate	150^[29]	>0.1	/		/		/		20.47±2.59b		/		40.3±2.31a	薰衣草、玫瑰、甜味 Lavender, rose, sweet taste
香茅醇 Citronellol	100^[30]	>0.1	20.21±0.3c		19.08±0.92c		26.75±1.77a		22.09±2.66b		17.16±0.68d		17±0.3d	柑橘、花香、水果 Citrus, floral, fruit
香叶醇 Geraniol	20^[31]	>0.1	14.52±1.92c		/		19.01±0.03b		29.11±3.15a		/		/	花香、百香果 Floral, passion fruit
反式-橙花叔醇 (E)-Nerolidol	70^[29]	>0.1	2.84±0.89c		/		5.68±0.12b		2.48±0.27c		10.8±1.64a		10.97±0.04a	花、蜡、木香 Flower, wax, wood fragrance
金合欢醇 Farnesol	20^[29]	>0.1	4.73±0.44c		/		6.89±0.12a		2.96±0.05d		5.35±0.45b		6.71±0.12a	花香、油味、甜味 Floral, oily, sweet flavors
C₁₃降异戊二烯C₁₃-norbornadiene
β-大马士酮 β-Damascone	0.05^[32]	>1.0	32.74±0.86d		32.17±0.54d		41.84±0.35c		45.38±0.44a		44.37±1.72ab		43.94±0.26b	花香、果香、蜂蜜 Floral, fruity, honey
C₆化合物C₆ compounds
正己醇 1-Hexanol	1100^[32]	>0.1	382.05±20.64b		401.35±1.69a		287.02±24.53c		277.96±9.8c		210.24±16.06d		207.8±3.19d	花、水果、生青味 Flowers, fruits, green flavors

Table 2

Fig. 9

Fig. 10

References 51

[1]	MOREIRA N, MENDES F, GUEDES DE PINHO P, HOGG T, VASCONCELOS I. Heavy sulphur compounds, higher alcohols and esters production profile of Hanseniaspora uvarum and Hanseniaspora guilliermondii grown as pure and mixed cultures in grape must. International Journal of Food Microbiology, 2008, 124(3): 231-238.
[2]	DEL FRESNO J M, MORATA A, LOIRA I, BAÑUELOS M A, ESCOTT C, BENITO S, GONZÁLEZ CHAMORRO C, SUÁREZ- LEPE J A. Use of non-Saccharomyces in single-culture, mixed and sequential fermentation to improve red wine quality. European Food Research and Technology, 2017, 243(12): 2175-2185.
[3]	RENAULT P, MIOT-SERTIER C, MARULLO P, HERNÁNDEZ- ORTE P, LAGARRIGUE L, LONVAUD-FUNEL A, BELY M. Genetic characterization and phenotypic variability in Torulaspora delbrueckii species: potential applications in the wine industry. International Journal of Food Microbiology, 2009, 134(3): 201-210.
[4]	COMITINI F, GOBBI M, DOMIZIO P, ROMANI C, LENCIONI L, MANNAZZU I, CIANI M. Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiology, 2011, 28(5): 873-882.
[5]	MEDINA K, BOIDO E, FARIÑA L, GIOIA O, GOMEZ M E, BARQUET M, GAGGERO C, DELLACASSA E, CARRAU F. Increased flavour diversity of Chardonnay wines by spontaneous fermentation and co-fermentation with Hanseniaspora vineae. Food Chemistry, 2013, 141(3): 2513-2521.
[6]	WANG X C, LI A H, DIZY M, ULLAH N, SUN W X, TAO Y S. Evaluation of aroma enhancement for “Ecolly” dry white wines by mixed inoculation of selected Rhodotorula mucilaginosa and Saccharomyces cerevisiae. Food Chemistry, 2017, 228: 550-559.
[7]	ALBERGARIA H, ARNEBORG N. Dominance of Saccharomyces cerevisiae in alcoholic fermentation processes: Role of physiological fitness and microbial interactions. Applied Microbiology and Biotechnology, 2016, 100(5): 2035-2046.
[8]	CIANI M, CAPECE A, COMITINI F, CANONICO L, SIESTO G, ROMANO P. Yeast interactions in inoculated wine fermentation. Frontiers in Microbiology, 2016, 7: 555. doi: 10.3389/fmicb.2016.00555 pmid: 27148235
[9]	CIANI M, COMITINI F. Yeast interactions in multi-starter wine fermentation. Current Opinion in Food Science, 2015, 1: 1-6.
[10]	NISSEN P, NIELSEN D, ARNEBORG N. Viable Saccharomyces cerevisiae cells at high concentrations cause early growth arrest of non-Saccharomyces yeasts in mixed cultures by a cell-cell contact- mediated mechanism. Yeast, 2003, 20(4): 331-341.
[11]	NISSEN P, NIELSEN D, ARNEBORG N. The relative glucose uptake abilities of non-Saccharomyces yeasts play a role in their coexistence with Saccharomyces cerevisiae in mixed cultures. Applied Microbiology and Biotechnology, 2004, 64(4): 543-550.
[12]	RENAULT P E, ALBERTIN W, BELY M. An innovative tool reveals interaction mechanisms among yeast populations under oenological conditions. Applied Microbiology and Biotechnology, 2013, 97(9): 4105-4119. doi: 10.1007/s00253-012-4660-5 pmid: 23292550
[13]	KEMSAWASD V, BRANCO P, ALMEIDA M G, CALDEIRA J, ALBERGARIA H, ARNEBORG N. Cell-to-cell contact and antimicrobial peptides play a combined role in the death of Lachanchea thermotolerans during mixed-culture alcoholic fermentation with Saccharomyces cerevisiae. FEMS Microbiology Letters, 2015, 362(14): fnv103.
[14]	LUYT N A, BEAUFORT S, DIVOL B, SETATI M E, TAILLANDIER P, BAUER F F. Phenotypic characterization of cell-to-cell interactions between two yeast species during alcoholic fermentation. World Journal of Microbiology and Biotechnology, 2021, 37(11): 186.
[15]	ENGLEZOS V, RANTSIOU K, GIACOSA S, RÍO SEGADE S, ROLLE L, COCOLIN L. Cell-to-cell contact mechanism modulates Starmerella bacillaris death in mixed culture fermentations with Saccharomyces cerevisiae. International Journal of Food Microbiology, 2019, 289: 106-114.
[16]	PIETRAFESA A, CAPECE A, PIETRAFESA R, BELY M, ROMANO P. Saccharomyces cerevisiae and Hanseniaspora uvarum mixed starter cultures: Influence of microbial/physical interactions on wine characteristics. Yeast, 2020, 37(11): 609-621.
[17]	PETITGONNET C, KLEIN G L, ROULLIER-GALL C, SCHMITT- KOPPLIN P, QUINTANILLA-CASAS B, VICHI S, JULIEN-DAVID D, ALEXANDRE H. Influence of cell-cell contact between L. thermotolerans and S. cerevisiae on yeast interactions and the exo-metabolome. Food Microbiology, 2019, 83: 122-133.
[18]	HU K, ZHAO H Y, EDWARDS N, PEYER L, TAO Y S, ARNEBORG N. The effects of cell-cell contact between Pichia kluyveri and Saccharomyces cerevisiae on amino acids and volatiles in mixed culture alcoholic fermentations. Food Microbiology, 2022, 103: 103960.
[19]	贾世宽, 刘亚琼, 陈佳威, 杨学威, 傅晓方, 王颉. 戴尔有孢圆酵母与酿酒酵母混合发酵对马瑟兰干红葡萄酒风味品质的影响. 中国食品学报, 2023, 23(10): 157-166.
	JIA S K, LIU Y Q, CHEN J W, YANG X W, FU X F, WANG J. Effects of mixed fermentation of Torulaspora delbrueckii and Saccharomyces cerevisiae on flavor quality of marselan dry red wine. Journal of Chinese Institute of Food Science and Technology, 2023, 23(10): 157-166. (in Chinese)
[20]	战吉宬, 曹梦竹, 游义琳, 黄卫东. 非酿酒酵母在葡萄酒酿造中的应用. 中国农业科学, 2020, 53(19): 4057-4069. doi: 10.3864/j.issn.0578-1752.2020.19.018.
	ZHAN J C, CAO M Z, YOU Y L, HUANG W D. Research advance on the application of non-Saccharomyces in winemaking. Scientia Agricultura Sinica, 2020, 53(19): 4057-4069. doi: 10.3864/j.issn.0578-1752.2020.19.018. (in Chinese)
[21]	BALMASEDA A, ROZÈS N, BORDONS A, REGUANT C. The use of Torulaspora delbrueckii to improve malolactic fermentation. Microbial Biotechnology, 2024, 17(1): e14302.
[22]	祝霞, 刘琦, 赵丹丹, 王璐璐, 韩舜愈, 杨学山. 酿造条件对酿酒酵母发酵香气的影响. 食品科学, 2019, 40(16): 115-123. doi: 10.7506/spkx1002-6630-20180929-321
	ZHU X, LIU Q, ZHAO D D, WANG L L, HAN S Y, YANG X S. Effect of different winemaking conditions on fermentation aroma production by Saccharomyces cerevisiae. Food Science, 2019, 40(16): 115-123. (in Chinese) doi: 10.7506/spkx1002-6630-20180929-321
[23]	PERRONE B, GIACOSA S, ROLLE L, COCOLIN L, RANTSIOU K. Investigation of the dominance behavior of Saccharomyces cerevisiae strains during wine fermentation. International Journal of Food Microbiology, 2013, 165(2): 156-162.
[24]	中华人民共和国国家质量监督检验检疫总局. GB/T15038—2006葡萄酒、果酒通用分析方法. 北京: 中国标准出版社, 2006.
	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. GB/T15038-2006 Analytical methods of wine and fruit wine. Beijing: Standards Press of China, 2006. (in Chinese)
[25]	JAYAPRAKASHA G K, SINGH R P, SAKARIAH K K. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chemistry, 2001, 73(3): 285-290.
[26]	房玉林, 孟江飞, 张昂, 张振文, 刘金串, 韩国民, 刘树文. 罐储时间对赤霞珠葡萄酒中酚类化合物及抗氧化活性的影响. 食品科学, 2011, 32(11): 14-20. doi: 10.7506/spkx1002-6630-201111004
	FANG Y L, MENG J F, ZHANG A, ZHANG Z W, LIU J C, HAN G M, LIU S W. Effect of storage time on phenolic components and antioxidant activity of red wine. Food Science, 2011, 32(11): 14-20. (in Chinese)
[27]	郑秋玲, 谭玉超, 肖慧琳, 贺长映, 刘万好, 王建萍, 刘笑宏, 唐美玲, 杜远鹏, 宫磊, 宋志忠, 卢建声, 徐维华. 不同砧木对赤霞珠干红葡萄酒花色苷及其抗氧化活力的影响. 中国酿造, 2021, 40(8): 105-109. doi: 10.11882/j.issn.0254-5071.2021.08.019
	ZHENG Q L, TAN Y C, XIAO H L, HE C Y, LIU W H, WANG J P, LIU X H, TANG M L, DU Y P, GONG L, SONG Z Z, LU J S, XU W H. Effects of different rootstocks on anthocyanins and antioxidant activity of Cabernet Sauvignon dry red wine. China Brewing, 2021, 40(8): 105-109. (in Chinese) doi: 10.11882/j.issn.0254-5071.2021.08.019
[28]	祝霞, 赵丹丹, 李俊娥, 毛亚玲, 韩舜愈, 杨学山. O. oeni糖苷酶活性对干白葡萄酒萜烯类香气的影响. 农业机械学报, 2021, 52(8): 363-373.
	ZHU X, ZHAO D D, LI J E, MAO Y L, HAN S Y, YANG X S. Effect of glycosidase activity of Oenococcus oeni on terpene aroma compounds of dry white wine. Transactions of the Chinese Society for Agricultural Machinery, 2021, 52(8): 363-373. (in Chinese)
[29]	马腾臻, 宫鹏飞, 史肖, 牛见明, 王春霞, 翟文娟, 张波, 韩舜愈. 红枣发酵酒香气成分分析及感官品质评价. 食品科学, 2021, 42(4): 247-253.
	MA T Z, GONG P F, SHI X, NIU J M, WANG C X, ZHAI W J, ZHANG B, HAN S Y. Aroma components and sensory properties of fermented jujube wine. Food Science, 2021, 42(4): 247-253. (in Chinese) doi: 10.7506/spkx1002-6630-20200131-293
[30]	FAÚNDEZ C, PÉREZ R, RAVANAL M C, EYZAGUIRRE J. Penicillium purpurogenum produces a novel, acidic, GH 3 beta- xylosidase: Heterologous expression and characterization of the enzyme. Carbohydrate Research, 2019, 482: 107738.
[31]	SÁENZ-NAVAJAS M P, AVIZCURI J M, BALLESTER J, FERNÁNDEZ-ZURBANO P, FERREIRA V, PEYRON D, VALENTIN D. Sensory-active compounds influencing wine experts' and consumers' perception of red wine intrinsic quality. LWT-Food Science and Technology, 2015, 60(1): 400-411.
[32]	MATTHEWS A, GRBIN P R, JIRANEK V. Biochemical characterisation of the esterase activities of wine lactic acid bacteria. Applied Microbiology and Biotechnology, 2007, 77(2): 329-337. pmid: 17828602
[33]	王倩倩, 覃杰, 马得草, 陶永胜. 优选发酵毕赤酵母与酿酒酵母混合发酵增香酿造爱格丽干白葡萄酒. 中国农业科学, 2018, 51(11): 2178-2192. doi: 10.3864/j.issn.0578-1752.2018.11.015.
	WANG Q Q, QIN J, MA D C, TAO Y S. Aroma enhancement of ecolly dry white wine by co-inoculation of selected Pichia fermentans and Saccharomyces cerevisiae. Scientia Agricultura Sinica, 2018, 51(11): 2178-2192. doi: 10.3864/j.issn.0578-1752.2018.11.015. (in Chinese)
[34]	ENGLEZOS V, RANTSIOU K, TORCHIO F, ROLLE L, GERBI V, COCOLIN L. Exploitation of the non-Saccharomyces yeast Starmerella bacillaris (synonym Candida zemplinina) in wine fermentation: Physiological and molecular characterizations. International Journal of Food Microbiology, 2015, 199: 33-40.
[35]	BRANCO P, FRANCISCO D, CHAMBON C, HÉBRAUD M, ARNEBORG N, ALMEIDA M G, CALDEIRA J, ALBERGARIA H. Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions. Applied Microbiology and Biotechnology, 2014, 98(2): 843-853.
[36]	李卉, 王颉, 吕烨, 徐立强, 郭海枫. 超声波催陈对干红葡萄酒感官指标、花色素、色度、色调和总酸浓度变化的影响. 河北农业大学学报, 2007, 30(4): 114-120.
	LI H, WANG J, LÜ Y, XU L Q, GUO H F. The influence of ultrasonic aging on the organoleptic character, anthocyanins, chromaticity, tonality and total acidity of dry red wine. Journal of Hebei Agricultural University, 2007, 30(4): 114-120. (in Chinese)
[37]	任学梅. 高产风味酶非酿酒酵母筛选、鉴定及其在葡萄酒增香酿造中的应用[D]. 兰州: 甘肃农业大学, 2022.
	REN X M. Screening and identification of non-Saccharomyces cerevisiae with high yield of flavor enzyme and its application in wine flavoring brewing[D]. Lanzhou: Gansu Agricultural University, 2022. (in Chinese)
[38]	彭昕, 杨兴元, 艾赛提∙阿合旦, 杨凡, 李泽涵, 李函伦. 游离SO₂、溶解氧浓度对葡萄酒颜色及花色苷含量的影响. 食品与机械, 2022, 38(10): 11-16.
	PENG X, YANG X Y, ASAT A, YANG F, LI Z H, LI H L. Effects of different concentrations of free SO₂and dissolved oxygen on wine color and anthocyanins content. Food & Machinery, 2022, 38(10): 11-16. (in Chinese)
[39]	RENAULT P, COULON J, DE REVEL G, BARBE J C, BELY M. Increase of fruity aroma during mixed T. delbrueckii/S. cerevisiae wine fermentation is linked to specific esters enhancement. International Journal of Food Microbiology, 2015, 207: 40-48.
[40]	TONDINI F, LANG T, CHEN L, HERDERICH M, JIRANEK V. Linking gene expression and oenological traits: Comparison between Torulaspora delbrueckii and Saccharomyces cerevisiae strains. International Journal of Food Microbiology, 2019, 294: 42-49.
[41]	ENGLEZOS V, RANTSIOU K, CRAVERO F, TORCHIO F, GIACOSA S, ORTIZ-JULIEN A, GERBI V, ROLLE L, COCOLIN L. Volatile profiles and chromatic characteristics of red wines produced with Starmerella bacillaris and Saccharomyces cerevisiae. Food Research International, 2018, 109: 298-309.
[42]	BENITO S, HOFMANN T, LAIER M, LOCHBÜHLER B, SCHÜTTLER A, EBERT K, FRITSCH S, RÖCKER J, RAUHUT D. Effect on quality and composition of Riesling wines fermented by sequential inoculation with non-Saccharomyces and Saccharomyces cerevisiae. European Food Research and Technology, 2015, 241(5): 707-717.
[43]	耿仕瑾, 姜娇, 曲睿, 石侃, 秦义, 刘延琳, 宋育阳. 戴尔有孢圆酵母调控晚采小芒森葡萄酒乙酸和香气. 农业工程学报, 2021, 37(7): 293-300.
	GENG S J, JIANG J, QU R, SHI K, QIN Y, LIU Y L, SONG Y Y. Managing volatile acidity and aroma of Petit Manseng wine using Torulaspora delbruekii. Transactions of the Chinese Society of Agricultural Engineering, 2021, 37(7): 293-300. (in Chinese)
[44]	SADOUDI M, TOURDOT-MARÉCHAL R, ROUSSEAUX S, STEYER D, GALLARDO-CHACÓN J J, BALLESTER J, VICHI S, GUÉRIN-SCHNEIDER R, CAIXACH J, ALEXANDRE H. Yeast- yeast interactions revealed by aromatic profile analysis of Sauvignon Blanc wine fermented by single or co-culture of non- Saccharomyces and Saccharomyces yeasts. Food Microbiology, 2012, 32(2): 243-253.
[45]	张文静, 杨诗妮, 杜爽, 姜娇, 叶冬青, 刘延琳. 本土毕赤克鲁维酵母与酿酒酵母混合发酵葡萄酒的增香潜力分析. 食品科学, 2020, 41(12): 84-90. doi: 10.7506/spkx1002-6630-20190418-232
	ZHANG W J, YANG S N, DU S, JIANG J, YE D Q, LIU Y L. Potential application of mixed starter cultures of indigenous Pichia kluyveri and Saccharomyces cerevisiae to wine aroma enhancement. Food Science, 2020, 41(12): 84-90. (in Chinese)
[46]	DUTRAIVE O, BENITO S, FRITSCH S, BEISERT B, PATZ C D, RAUHUT D. Effect of sequential inoculation with non- Saccharomyces and Saccharomyces yeasts on Riesling wine chemical composition. Fermentation, 2019, 5(3): 79.
[47]	夏鸿川, 张众, 孙丽君, 李伟, 张军翔. 混菌发酵对贺兰山东麓‘赤霞珠’干红葡萄酒香气的影响. 食品科学, 2022, 43(14): 165-175.
	XIA H C, ZHANG Z, SUN L J, LI W, ZHANG J X. Effect of mixed culture fermentations on the aroma compounds of ‘Cabernet Sauvignon’ dry red wine produced in the eastern foothill of Helan Mountain. Food Science, 2022, 43(14): 165-175. (in Chinese) doi: 10.7506/spkx1002-6630-20210710-099
[48]	HU K, JIN G J, XU Y H, TAO Y S. Wine aroma response to different participation of selected Hanseniaspora uvarum in mixed fermentation with Saccharomyces cerevisiae. Food Research International, 2018, 108: 119-127.
[49]	ZHANG B Q, LUAN Y, DUAN C Q, YAN G L. Use of Torulaspora delbrueckii co-fermentation with two Saccharomyces cerevisiae strains with different aromatic characteristic to improve the diversity of red wine aroma profile. Frontiers in Microbiology, 2018, 9: 606.
[50]	PÉREZ D, DENAT M, MINEBOIS R, HERAS J M, GUILLAMÓN J M, FERREIRA V, QUEROL A. Modulation of aroma and chemical composition of Albariño semi-synthetic wines by non- wine Saccharomyces yeasts and bottle aging. Food Microbiology, 2022, 104: 103981.
[51]	王伟雄. 基于Kluyveromyces marxianus与酿酒酵母混合发酵葡萄酒动力学分析及质量提升研究[D]. 乌鲁木齐: 新疆农业大学, 2022.
	WANG W X. Dynamic analysis and quality improvement of mixed fermentation wine based on Kluyveromyces marxianus and Saccharomyces cerevisiae[D]. Urumqi: Xinjiang Agricultural University, 2022. (in Chinese)

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发酵方式 Fermentation method	酵母组合 Yeast combination	同时接种 Simultaneous inoculation		顺序接种 Sequential inoculation
发酵方式 Fermentation method	酵母组合 Yeast combination	0 h		0 h	48 h
纯种发酵 Pure fermentation	ES488	*
纯种发酵 Pure fermentation	Td	*
混菌发酵 Mixed fermentation	ES488/Td	+	x	+	x
双室发酵 Double-compartment fermentation	Td（左 Left）/ES488（右 Right）	+	x	+	x

Effects of Cell-to-Cell Contact Between Torulaspora delbrueckii and Saccharomyces cerevisiae on the Flavor and Quality of Cabernet Sauvignon Wine

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