枸橼C-05 CmPR4A上游转录因子CmWRKY75的筛选及其抗溃疡病功能分析

doi:10.3864/j.issn.0578-1752.2023.21.013

Abstract

Abstract:

【Background】Citrus canker is one of the serious citrus diseases caused by Xanthomonas citri subsp. citri (Xcc). There is currently no radical cure method for it, and few of the existing cultivars have sufficient resistance to citrus canker. Therefore, the breeding for resistant varieties is crucial for the radical cure of the disease, and the identification of resistant genes is beneficial to disease-resistant cultivar breeding.【Objective】The aim of this study was to use the resistance related gene CmPR4A to screen its upstream transcription factors, and to explore the role of transcription factors in resistance to Xcc, which could provide genetic information for the breeding of citrus disease resistant varieties.【Method】Based on the transcriptome results of Citron C-05 (resistant) and Bingtang Sweet orange (susceptible) after inoculated with Xcc, and combined with the results of qRT-PCR, PR4A was differentially expressed in resistant and susceptible genotypes. Differential analysis on PR4A promoter sequence of Citron C-05 and Bingtang Sweet orange was performed using PlantCARE. Yeast one hybrid was used to screen the upstream transcription factors of PR4A. Further interaction between CmPR4A and candidate transcription factors was verified by yeast gyration test and dual-Luciferase. The expression of candidate transcription factors was detected among 8 resistant and susceptible citrus genotypes after inoculation with Xcc at 0, 2, 4, 6, and 8 days to verify their relationship with disease resistance. By transient overexpression of candidate transcription factors in Citron C-05 and Bingtang sweet orange leaves, the expression of transcription factors and PR4A were analyzed using qRT-PCR. Xcc bacterial quantification and symptom observation were executed in transgenic leaves after 24 h inoculated with Xcc.【Result】The expression of PR4A was significantly higher in resistant Citron C-05 than that in the susceptible Bingtang sweet orange at 4, 6, and 8 dpi after inoculated with Xcc. There was a difference in the cis acting element W-box in PR4A promoter between Citron C-05 and Bingtang sweet orange at -236 bp location. Therefore, the CmPR4A promoter was truncated and the bait vector was constructed. Yeast one hybrid screening was conducted using Citron C-05 yeast library induced by Xcc, resulting in CmWRKY75 could interact with proCmPR4A-2. Further dual Luciferase reporting system also confirmed that the interaction between CmWRKY75 and CmPR4A, and CmWRKY75 was positive regulating the expression of CmPR4A. Additionally, the expression of WRKY75 was significantly upregulated in resistant genotypes Citron C-05, American citron and Aiguo citron after inoculation with Xcc, while it was only slight upregulation in susceptible genotypes Bingtang Sweet orange, Shatian Yu pummelo, lemon, Nanchuan and Danna citron. Transient overexpression WRKY75 in Citron C-05 and Bingtang Sweet orange leaves revealed a significant upregulation expression of PR4A at 4 dpi of Xcc and enhanced leaf resistance to Xcc.【Conclusion】CmWRKY75 could bind to the W-box in CmPR4A promoter and positively regulate the expression of CmPR4A, resulting in enhancing leaf resistance to Xcc. Moreover, the expression of WRKY75 was induced by Xcc and showed significant upregulation in disease-resistant genotypes, which was consistent with the expression pattern of PR4A. These results indicated that the differential expression of WRKY75 in different disease-resistant and susceptible citrus genotypes influenced the expression of PR4A, which made it play a role in the resistance of Citron C-05 to canker disease.

Key words: citrus canker disease, Citron C-05, PR4A, transcription factor, WRKY75

YAN PeiHan, LUO JianMing, HAO ChenXing, SUN ZiQing, YE RongChun, LI Yi, LIU Lian, SHENG Ling, MA XianFeng, DENG ZiNiu. Identification of the Transcription Factor WRKY75 of CmPR4A in Citron C-05 and Its Function Analysis in Resistance to Citrus Canker Disease[J].Scientia Agricultura Sinica, 2023, 56(21): 4304-4317.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2023.21.013

https://www.chinaagrisci.com/EN/Y2023/V56/I21/4304

Figures/Tables 10

Table 1

Fig. 1

Fig. 2

Table 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

References 44

[1]	SUDYOUNG N, TOKUYAMA S, KRAJANGSANG S, PRINGSULAKA O, SARAWANEEYARUK S. Bacterial antagonists and their cell-free cultures efficiently suppress canker disease in citrus lime. Journal of Plant Diseases and Protection, 2020, 127(2): 173-181. doi: 10.1007/s41348-019-00295-9
[2]	BEHLAU F, GOCHEZ A M, JONES J B. Diversity and copper resistance of Xanthomonas affecting citrus. Tropical Plant Pathology, 2020, 45(3): 200-212. doi: 10.1007/s40858-020-00340-1
[3]	GOCHEZ A M, BEHLAU F, SINGH R, ONG K, WHILBY L, JONES J B. Panorama of citrus canker in the United States. Tropical Plant Pathology, 2020, 45(3): 192-199. doi: 10.1007/s40858-020-00355-8
[4]	FERENCE CHRISTOPHER M, GOCHEZ ALBERTO M, FRANKLIN B, NIAN W, GRAHAM JAMES H, JONES JEFFREY B. Recent advances in the understanding of Xanthomonas citri ssp. citri pathogenesis and citrus canker disease management. Molecular Plant Pathology, 2018, 19(6): 1302-1318. doi: 10.1111/mpp.2018.19.issue-6
[5]	DENG Z N, XU L, LI D Z, LONG G Y, LIU L P, FANG F, SHU G P. Screening citrus genotypes for resistance to canker disease (Xanthomonas axonopodis pv. citri). Plant Breeding, 2009, 129(3): 341-345. doi: 10.1111/pbr.2010.129.issue-3
[6]	郝晨星. 响应柑橘溃疡病菌侵染的枸橼C-05抗性基因PR4A的鉴定[D]. 长沙: 湖南农业大学, 2019.
	HAO C X. Identification of Citrus citrus C-05 resistance gene PR4A in response to Citrus ulcer infection[D]. Changsha: Hunan Agricultural University, 2019. (in Chinese)
[7]	DANGL J L, JONES J D G. Plant pathogens and integrated defence responses to infection. Nature, 2001, 411(6839): 826-833. doi: 10.1038/35081161
[8]	YUAN M H, NGOU B P M, DING P T, XIN X F. PTI-ETI crosstalk: An integrative view of plant immunity. Current Opinion in Plant Biology, 2021, 62: 102030. doi: 10.1016/j.pbi.2021.102030
[9]	SELS J, MATHYS J, DE CONINCK B M A, CAMMUE B P A, DE BOLLE M F C. Plant pathogenesis-related (PR) proteins: A focus on PR peptides. Plant Physiology and Biochemistry, 2008, 46(11): 941-950. doi: 10.1016/j.plaphy.2008.06.011 pmid: 18674922
[10]	刘红霞, 赵晨辉, 刘洋, 李增海, 梁英海, 张冰冰. 植物系统获得抗病性及其信号调控. 吉林农业科学, 2012, 37(2): 38-41, 51.
	LIU H X, ZHAO C H, LIU Y, LI Z H, LIANG Y H, ZHANG B B. Systematic acquired resistance (SAR) of plant and its signal regulation. Journal of Jilin Agricultural Sciences, 2012, 37(2): 38-41, 51. (in Chinese)
[11]	张玉, 杨爱国, 冯全福, 蒋彩虹, 耿锐梅, 罗成刚. 植物病程相关蛋白及其在烟草中的研究进展. 生物技术通报, 2012(5): 20-24.
	ZHANG Y, YANG A G, FENG Q F, JIANG C H, GENG R M, LUO C G. Plant pathogenesis-related proteins and research progress in tobacco. Biotechnology Bulletin, 2012(5): 20-24. (in Chinese)
[12]	ALEXANDER D, GOODMAN R M, GUT-RELLA M, GLASCOCK C, WEYMANN K, FRIEDRICH L, MADDOX D, AHL-GOY P, LUNTZ T, WARD E. Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a. Proceedings of the National Academy of Sciences of the United States of America, 1993, 90(15): 7327-7331.
[13]	MUTHUKRISHNAN S, LIANG G H, TRICK H N, GILL B S. Pathogenesis-related proteins and their genes in cereals. Plant Cell, Tissue and Organ Culture, 2001, 64(2): 93-114. doi: 10.1023/A:1010763506802
[14]	AWADE A, DE TAPIA M, DIDIERJEAN L, BURKARD G. Biological function of bean pathogenesis-related (PR 3 and PR 4) proteins. Plant Science, 1989, 63(2): 121-130. doi: 10.1016/0168-9452(89)90237-9
[15]	KIM Y J, LEE H J, JANG M G, KWON W S, KIM S Y, YANG D C. Cloning and characterization of pathogenesis-related protein 4 gene from Panax ginseng. Russian Journal of Plant Physiology, 2014, 61(5): 664-671. doi: 10.1134/S1021443714050100
[16]	HWANG I S, CHOI D S, KIM N H, KIM D S, HWANG B K. Pathogenesis-related protein 4b interacts with leucine-rich repeat protein1 to suppress PR4b-triggered cell death and defense response in pepper. The Plant Journal, 2014, 77(4): 521-533. doi: 10.1111/tpj.2014.77.issue-4
[17]	REGLINSKI T, VANNESTE J L, SCHIPPER M M, CORNISH D A, YU J, OLDHAM J M, FEHLMANN C, PARRY F, HEDDERLEY D. Postharvest application of acibenzolar-S-methyl activates salicylic acid pathway genes in kiwifruit vines. Plants, 2023, 12(4): 833. doi: 10.3390/plants12040833
[18]	ZHAO J Y, CHEN J, SHI Y, FU H Y, HUANG M T, ROTT P C, GAO S J. Sugarcane responses to two strains of Xanthomonas albilineans differing in pathogenicity through a differential modulation of salicylic acid and reactive oxygen species. Frontiers in Plant Science, 2022, 13: 1087525. doi: 10.3389/fpls.2022.1087525
[19]	SEO P J, LEE A K, XIANG F N, PARK C M. Molecular and functional profiling of Arabidopsis pathogenesis-related genes: Insights into their roles in salt response of seed germination. Plant and Cell Physiology, 2008, 49(3): 334-344. doi: 10.1093/pcp/pcn011
[20]	PONSTEIN A S, BRES-VLOEMANS S A, SELA-BUURLAGE M B, VAN DEN ELZEN P J, MELCHERS L S, CORNELISSEN B J. A novel pathogen- and wound-inducible tobacco (Nicotiana tabacum) protein with antifungal activity. Plant Physiology, 1994, 104(1): 109-118. pmid: 8115541
[21]	GUEVARA-MORATO M Á, GARCÍA DE LACOBA M, GARCÍA- LUQUE I, SERRA M T. Characterization of a pathogenesis-related protein 4 (PR-4) induced in Capsicum chinense L3 plants with dual RNase and DNase activities. Journal of Experimental Botany, 2010, 61(12): 3259-3271. doi: 10.1093/jxb/erq148
[22]	DAI L M, WANG D, XIE X Q, ZHANG C H, WANG X P, XU Y, WANG Y J, ZHANG J X. The novel gene VpPR4-1 from Vitis pseudoreticulata increases powdery mildew resistance in transgenic Vitis vinifera L. Frontiers in Plant Science, 2016, 7: 695.
[23]	ZHOU Z, ZHU Y M, TIAN Y, YAO J L, BIAN S X, ZHANG H T, ZHANG R P, GAO Q M, YAN Z L. MdPR4, a pathogenesis-related protein in apple, is involved in chitin recognition and resistance response to apple replant disease pathogens. Journal of Plant Physiology, 2021, 260: 153390. doi: 10.1016/j.jplph.2021.153390
[24]	RUSHTON P J, SOMSSICH I E, RINGLER P, SHEN Q J. WRKY transcription factors. Trends in Plant Science, 2010, 15(5): 247-258. doi: 10.1016/j.tplants.2010.02.006 pmid: 20304701
[25]	ZHANG L P, CHEN L G, YU D Q. Transcription factor WRKY75 interacts with DELLA proteins to affect flowering. Plant Physiology, 2018, 176(1): 790-803. doi: 10.1104/pp.17.00657 pmid: 29133369
[26]	CHEN L G, ZHANG L P, XIANG S Y, CHEN Y L, ZHANG H Y, YU D Q. The transcription factor WRKY75 positively regulates jasmonate-mediated plant defense to necrotrophic fungal pathogens. Journal of Experimental Botany, 2021, 72(4): 1473-1489. doi: 10.1093/jxb/eraa529 pmid: 33165597
[27]	ZHANG H Y, ZHANG L P, JI Y R, JING Y F, LI L X, CHEN Y L, WANG R L, ZHANG H M, YU D Q, CHEN L G. Arabidopsis sigma factor binding protein₁ (sib1) and sib2 inhibit wrky75 function in abscisic acid-mediated leaf senescence and seed germination. Journal of Experimental Botany, 2022, 73(1): 182-196. doi: 10.1093/jxb/erab391
[28]	LIU J Q, CHEN X J, LIANG X X, ZHOU X G, YANG F, LIU J, HE S Y, GUO Z J. Alternative splicing of rice WRKY62 and WRKY76 transcription factor genes in pathogen defense. Plant Physiology, 2016, 171(2): 1427-1442. doi: 10.1104/pp.15.01921 pmid: 27208272
[29]	WANG X H, GUO R R, TU M X, WANG D J, GUO C L, WAN R, LI Z, WANG X P. Ectopic expression of the wild grape WRKY transcription factor VqWRKY52 in Arabidopsis thaliana enhances resistance to the biotrophic pathogen powdery mildew but not to the necrotrophic pathogen Botrytis cinerea. Frontiers in Plant Science, 2017, 8: 97-110.
[30]	LIU Y S, LIU Q W, LI X W, ZHANG Z J, AI S K, LIU C, MA F W, LI C. MdERF114 enhances the resistance of apple roots to Fusarium solani by regulating the transcription of MdPRX63. Plant Physiology, 2023, 192(3): 2015-2029. doi: 10.1093/plphys/kiad057
[31]	QIU D Y, XIAO J, XIE W B, CHENG H T, LI X H, WANG S P. Exploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice. BMC Plant Biology, 2009, 9: 74. doi: 10.1186/1471-2229-9-74 pmid: 19534828
[32]	LI M Y, JIAO Y T, WANG Y T, ZHANG N, WANG B B, LIU R Q, YIN X, XU Y, LIU G T. CRISPR/Cas9-mediated VvPR4b editing decreases downy mildew resistance in grapevine (Vitis vinifera L.). Horticulture Research, 2020, 7(1): 149-160. doi: 10.1038/s41438-020-00371-4
[33]	YANG T, WANG Y. Research progress of plant pathogenesis related protein PR-10. Plant Physiology Journal, 2017, 53: 2057-2068.
[34]	张远嬿. 苹果MdWRKY33基因的克隆与功能分析[D]. 沈阳: 沈阳农业大学, 2018.
	ZHANG Y Y. Cloning and functional analysis of apple MdWRKY33 gene[D]. Shenyang: Shenyang Agricultural University, 2018. (in Chinese)
[35]	周茜茜, 邱化荣, 何晓文, 王宪璞, 刘秀霞, 李保华, 吴树敬, 陈学森. MdWRKY40介导提高苹果与拟南芥对轮纹病菌的免疫抗性. 中国农业科学, 2018, 51(21): 4052-4064. doi: 10.3864/j.issn.0578-1752.2018.21.005.
	ZHOU Q Q, QIU H R, HE X W, WANG X P, LIU X X, LI B H, WU S J, CHEN X S. MdWRKY40 mediated improvement of the immune resistance of apple and Arabidopsis thaliana to Botryosphaeria dothidea. Scientia Agricultura Sinica, 2018, 51(21): 4052-4064. doi: 10.3864/j.issn.0578-1752.2018.21.005. (in Chinese)
[36]	LONG Q, DU M X, LONG J H, XIE Y, ZHANG J Y, XU L Z, HE Y R, LI Q, CHEN S C, ZOU X P. Transcription factor WRKY22 regulates canker susceptibility in sweet orange (Citrus sinensis Osbeck) by enhancing cell enlargement and CsLOB1 expression. Horticulture Research, 2021, 8(1): 50-65. doi: 10.1038/s41438-021-00486-2
[37]	DENG B, WANG W J, RUAN C Q, DENG L L, YAO S X, ZENG K F. Involvement of CsWRKY70 in salicylic acid-induced citrus fruit resistance against Penicillium digitatum. Horticulture Research, 2020, 7: 157. doi: 10.1038/s41438-020-00377-y
[38]	GUO P R, LI Z H, HUANG P X, LI B S, FANG S, CHU J F, GUO H W. A tripartite amplification loop involving the transcription factor WRKY75, salicylic acid, and reactive oxygen species accelerates leaf senescence. The Plant Cell, 2017, 29(11): 2854-2870. doi: 10.1105/tpc.17.00438 pmid: 29061866
[39]	卢婷, 杨莉, 胡威, 匡柳青, 郭文芳, 沈丹, 刘德春, 刘勇. 柑橘抗逆基因WRKY75的克隆与表达分析. 江西农业大学学报(自然科学版), 2021, 43(1): 82-93.
	LU T, YANG L, HU W, KUANG L Q, GUO W F, SHEN D, LIU D C, LIU Y. Cloning and expression analysis of Citrus WRKY75 genes in response to abiotic stresses. Acta Agriculturae Universitatis Jiangxiensis (Natural Sciences Edition), 2021, 43(1): 82-93. (in Chinese)
[40]	张娜. 中国野生葡萄VpWRKY75调控下游靶基因抗霜霉病的功能研究[D]. 杨凌: 西北农林科技大学, 2021.
	ZHANG N. Study on the function of China wild grape VpWRKY75 in regulating downstream target genes to resist downy mildew[D]. Yangling: Northwest A & F University, 2021. (in Chinese)
[41]	TAN X L, FAN Z Q, LI L L, WU Y, KUANG J F, LU W J, CHEN J Y. Molecular characterization of a leaf senescence-related transcription factor BrWRKY75 of Chinese flowering cabbage. Horticultural Plant Journal, 2016, 2(5): 272-278. doi: 10.1016/j.hpj.2017.01.003
[42]	MALECK K, LEVINE A, EULGEM T, MORGAN A, SCHMID J, LAWTON K A, DANGL J L, DIETRICH R A. The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nature Genetics, 2000, 26(4): 403-410. doi: 10.1038/82521
[43]	MZID R, MARCHIVE C, BLANCARD D, DELUC L, BARRIEU F, CORIO-COSTET M, DRIRA N, HAMDI S, LAUVERGEAT V. Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens. Physiologia Plantarum, 2007, 131(3): 434-447. doi: 10.1111/ppl.2007.131.issue-3
[44]	刘兵, 李梦媛, 张娜, 尚博兴, 刘国甜, 徐炎. 中国野生葡萄抗霜霉病相关基因VpPR4b及其启动子的克隆和功能分析. 园艺学报, 2021, 48(2): 265-275. doi: 10.16420/j.issn.0513-353x.2020-0343
	LIU B, LI M Y, ZHANG N, SHANG B X, LIU G T, XU Y. Cloning and functional analysis of the CDS and promoter of VpPR4b gene response to downy mildew in Chinese wild grape. Acta Horticulturae Sinica, 2021, 48(2): 265-275. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

用途 Use	引物名称 Primer name	引物序列 Primer sequence (5′-3′)
酵母单杂交 Yeast one hybrid	proCmPR4A-P1-AbAi-F-Sac I	CTTGAATTCGAGCTCGGGTTGCTCAAGAGCCAGATG
	proCmPR4A-P2-AbAi -F-Sac I	CTTGAATTCGAGCTCCCCTTAGCAGCCCCCGC
	proCmPR4A-AbAi -R-Xho I	AGCACATGCCTCGAGATTTTTCTCTTAATTTTTTTTAGCTTAACTTACC
	CmWRKY75-pGADT7-F-EcoR I	GGAGGCCAGTGAATTCATGGAAAATTGCCAAATGTTTTTC
	CmWRKY75-pGADT7-R-Bam HI	CGAGCTCGATGGATCCTCAAAATGGAGTGTAAATTTGCAACT
双荧光素酶 Dual-luciferase	proCmPR4A-pGreen-F-Hind III	GACGGTATCGATAAGCTTCCCTTAGCAGCCCCCGC
双荧光素酶 Dual-luciferase	proCmPR4A-pGreen-R-Bam HI	AGAACTAGTGGATCCATTTTTCTCTTAATTTTTTTTAGCTTAACTTACC
瞬时过表达 Over-expression	CmWRKY75-P1300-F-Sac I	ACGGGGGACGAGCTCATGGAAAATTGCCAAATGTTTTTC
瞬时过表达 Over-expression	CmWRKY75-P1300-R-Kpn I	GCTCACCATGGTACCTCAAAATGGAGTGTAAATTTGCAACT
实时荧光定量PCR Quantitative Real-time PCR	Actin-qPCR -F	CATCCCTCAGCACCTTCC
	Actin-qPCR -R	CCAACCTTAGCACTTCTCC
	PR4A-qPCR-F	TATGGCTGGACCGCTTTCTG
	PR4A-qPCR-R	TCTAAGCCTCCATTGGCACA
	WRKY75-qPCR-F	TGCTTTCCAAACAAGAAGCCAA
	WRKY75-qPCR-R	CAACGGTAGTAGCTTCTGGGG

基因名称 Gene name	基因ID Gene ID	转录因子家族 Transcription factor family	功能 Function
HDG1	Cme072170	HD-ZIP	茉莉酸代谢；角质层发育；花器官特性的维持 Jasmonate metabolism; Development of cuticle; Maintenance of floral organ characteristics
MADA4	Cme017590	ZF-HD	细胞分化、防御反应、调节代谢 Cell differentiation, defense response, regulation of metabolism
RMA3	Cme174580	ZF-HD	解旋酶和E3泛素连接酶活性 Activity of helicase and E3 ubiquitin ligase
WRKY33	Cme018250	WRKY family	SUMO化修饰调控植物免疫反应 SUMO modification regulates plant immune response
bHLH122	Cme111260	bHLH	表皮发育；光周期；调节气孔运动 Epidermal development; Photoperiod; Regulate stomatal movement
TGA10	Cme058810	bZIP	调节植物信号分子水杨酸和茉莉酸甲酯 Regulate plant signal molecules salicylic acid and methyl jasmonate
bZIP 60	Cme155220	bZIP	调控种子萌发；增强耐热性 Regulating seed germination; Enhanced heat resistance
WRKY75	Cme125500	WRKY	参与叶片与花瓣的衰老与脱落；参与抗病反应 Participate in the senescence and shedding of leaves and petals; Participate in disease resistance response

Identification of the Transcription Factor WRKY75 of CmPR4A in Citron C-05 and Its Function Analysis in Resistance to Citrus Canker Disease

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 10

References 44

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LI Hui, ZHANG YuFeng, LI XiaoGang, WANG ZhongHua, LIN Jing, CHANG YouHong. Identification of Salt-Tolerant Transcription Factors in the Roots of Pyrus betulaefolia by the Association Analysis of Genome-Wide DNA Methylation and Transcriptome [J]. Scientia Agricultura Sinica, 2023, 56(7): 1377-1390.
[2]	LI YiPu, TONG LiXiu, LIN YaNan, SU ZhiJun, BAO HaiZhu, WANG FuGui, LIU Jian, QU JiaWei, HU ShuPing, SUN JiYing, WANG ZhiGang, YU XiaoFang, XU MingLiang, GAO JuLin. Investigation of Low Nitrogen Tolerance of ZmCCT10 in Maize [J]. Scientia Agricultura Sinica, 2023, 56(6): 1035-1044.
[3]	HAN XiaoWen, HAN Shuo, HU YiFeng, WANG MengRu, CHEN ZhongYi, ZHU YongXing, YIN JunLiang. Genome-Wide Identification of AP2/ERF Gene Family in Alternanthera philoxeroides and Its Expression Patterns Under Herbicide Stresses [J]. Scientia Agricultura Sinica, 2023, 56(20): 4021-4034.
[4]	DING GuoHua, XIAO GuangHui, ZHU LiPing. Genome-Wide Identification and Expression Analysis of NLP (NIN- Like Protein) Transcription Factor Gene Family in Cotton [J]. Scientia Agricultura Sinica, 2023, 56(19): 3723-3746.
[5]	ZHANG Nan, HAN GuangJie, LIU Qin, LI ChuanMing, QI JianHang, LU YuRong, XIA Yang, XU Jian. Response and Function of the Transcription Factor CncC in Cnaphalocrocis medinalis Infected with Baculovirus CnmeGV [J]. Scientia Agricultura Sinica, 2023, 56(13): 2491-2503.
[6]	YOU YuWan,ZHANG Yu,SUN JiaYi,ZHANG Wei. Genome-Wide Identification of NAC Family and Screening of Its Members Related to Prickle Development in Rosa chinensis Old Blush [J]. Scientia Agricultura Sinica, 2022, 55(24): 4895-4911.
[7]	ZHANG Jie, JIANG ChangYue, WANG YueJin. Functional Analysis of the Interaction Between Transcription Factors VqWRKY6 and VqbZIP1 in Regulating the Resistance to Powdery Mildew in Chinese Wild Vitis quinquangularis [J]. Scientia Agricultura Sinica, 2022, 55(23): 4626-4639.
[8]	PANG HaoWan,FU QianKun,YANG QingQing,ZHANG YuanYuan,FU FengLing,YU HaoQiang. Maize Transcription Factor ZmEREB93 Negatively Regulates Kernel Development [J]. Scientia Agricultura Sinica, 2022, 55(19): 3685-3696.
[9]	YANG ShengDi,MENG XiangXuan,GUO DaLong,PEI MaoSong,LIU HaiNan,WEI TongLu,YU YiHe. Co-Expression Network and Transcriptional Regulation Analysis of Sulfur Dioxide-Induced Postharvest Abscission of Kyoho Grape [J]. Scientia Agricultura Sinica, 2022, 55(11): 2214-2226.
[10]	LIU RuiDa, GE ChangWei, WANG MinXuan, SHEN YanHui, LI PengZhen, CUI ZiQian, LIU RuiHua, SHEN Qian, ZHANG SiPing, LIU ShaoDong, MA HuiJuan, CHEN Jing, ZHANG GuiYin, PANG ChaoYou. Cloning and Drought Resistance Analysis of Transcription Factor GhMYB108 in Gossypium hirsutum [J]. Scientia Agricultura Sinica, 2022, 55(10): 1877-1890.
[11]	MA ShuanHong, WAN Jiong, LIANG RuiQing, ZHANG XueHai, QIU XiaoQian, MENG ShuJun, XU NingKun, LIN Yuan, DANG KunTai, WANG QiYue, ZHAO JiaWen, DING Dong, TANG JiHua. Candidate Gene Association Analysis of Maize Transcription Factors in Flowering Time [J]. Scientia Agricultura Sinica, 2022, 55(1): 12-25.
[12]	LÜ ShiKai, MA XiaoLong, ZHANG Min, DENG PingChuan, CHEN ChunHuan, ZHANG Hong, LIU XinLun, JI WanQuan. Post-transcriptional Regulation of TaNAC Genes by Alternative Splicing and MicroRNA in Common Wheat (Triticum aestivum L.) [J]. Scientia Agricultura Sinica, 2021, 54(22): 4709-4727.
[13]	ZHU FangFang,DONG YaHui,REN ZhenZhen,WANG ZhiYong,SU HuiHui,KU LiXia,CHEN YanHui. Over-expression of ZmIBH1-1 to Improve Drought Resistance in Maize Seedlings [J]. Scientia Agricultura Sinica, 2021, 54(21): 4500-4513.
[14]	ZHANG JingYun,LIU YuNuo,WANG ZhaoHao,PENG AiHong,CHEN ShanChun,HE YongRui. Analysis of Resistance Mechanism of CiNPR4 Transgenic Plants to Citrus Canker [J]. Scientia Agricultura Sinica, 2021, 54(18): 3871-3880.
[15]	ZHAO JingYa,XIA HuiQing,PENG MengYa,FAN Zhuo,YIN Yue,XU SaiBo,ZHANG Nan,CHEN WenBo,CHEN LinLin. Identification and Functional Analysis of Transcription Factors FpAPSES in Fusarium pseudograminearum [J]. Scientia Agricultura Sinica, 2021, 54(16): 3428-3439.