黑土水溶性有机碳对有机物料还田的响应

doi:10.3864/j.issn.0578-1752.2020.06.010

Abstract

Abstract:

【Objective】The change characteristics of water soluble organic carbon (WSOC) content and components in black soil with the addition of different organic materials were explored to provide a scientific basis for soil fertility improvement methods in black soil area.【Method】This study was based on experimental plots of returning organic materials to the field in Keshan County, Heilongjiang Province, and the experiment has lasted for seven years. The content and structure of WSOC treated with organic fertilizer, biochar, straw and single fertilizer treatment (CK) was analyzed by routine determination and fluorescence analysis.【Result】Compared with the single application of chemical fertilizer, the content of soil water-soluble organic carbon increased by 9.65%-20.30% and soil organic carbon increased by 6.63%-14.86% under the application of organic materials. The contents of tyrosine-like protein and tryptophan-like protein in WSOC decreased under the treatment of organic materials. The application of organic fertilizer increased the contents of metabolites of soluble microorganisms in WSOC, increased the contents of fulvic acid and humic acid in WSOC, and simplified their structures. Straw and biochar simplified the structure of fulvic acids and biochar complicates the structure of humic acids in WSOC.【Conclusion】Organic fertilizer, biochar and straw improved the content of soil water-soluble organic carbon, enhanced the decomposition and metabolism of soil microorganisms, increased the content and simplified the structure of fulvic acid component in water-soluble organic carbon. Organic fertilizer had the best effect.

Key words: black soil, organic material, water-soluble organic carbon, three-dimensional fluorescence spectra, PARAFAC analysis

Dan WEI,ShanShan CAI,Yan LI,Liang JIN,Wei WANG,YuMei LI,Yang BAI,Yu HU. The Response of Water-Soluble Organic Carbon to Organic Material Applications in Black Soil[J].Scientia Agricultura Sinica, 2020, 53(6): 1180-1188.

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年份 Year	处理 Treatment	有机碳 TOC (g·kg^-1）	全氮 Total N (g·kg^-1)	全磷 Total P (g·kg^-1)	全钾 Total K (g·kg^-1)	碱解氮Available N (mg·kg^-1)	速效磷Available P (mg·kg^-1)	速效钾Available K (mg·kg^-1)	pH
2013	均值Mean	30.03	1.81	1.69	18.9	129.12	11.19	202.2	6.36
2019	NPK	27.76	1.81	1.16	18.30	85.7	27.69	172.4	6.19
	MNPK	30.78	2.28	1.52	23.40	106.64	33.35	286.3	6.49
	SNPK	30.45	2.04	1.30	22.10	97.48	20.86	234.7	6.69
	BNPK	30.06	1.97	1.01	23.30	91.59	27.13	198.3	6.51

处理 Treatment	SOC (g·kg^-1)	WSOC (mg·kg^-1)	WSOC/SOC (%)
NPK	28.10±0.69c	150.23±7.52b	0.53±0.10a
MNPK	32.28±0.66a	180.73±17.09a	0.56±0.06a
BNPK	30.29±0.73b	165.00±8.34ab	0.55±0.04a
SNPK	29.96±0.84b	164.73±4.19ab	0.55±0.02a

荧光区域 Fluorescence region	物质类型Types of substances	Ex (nm)	Em (nm)
Ⅰ	类酪氨酸蛋白质物质 Tyrosine-like protein	200-250	250-330
Ⅱ	类色氨酸蛋白质物质 Tryptophan-like protein	200-250	330-380
Ⅲ	类富里酸物质 Fulvic acid-like	200-250	380-550
Ⅳ	溶解性微生物代谢产物 Soluble microbial metabolites	250-490	250-380
Ⅴ	类腐殖酸物质 Humic-like	250-490	380-550

处理 Treatment	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
NPK	2.26±0.27a	3.49±0.17a	22.95±0.25a	7.00±0.04b	64.31±0.63a
MNPK	1.40±0.39b	3.37±0.14a	19.82±0.10c	11.04±0.27a	64.37±0.80a
BNPK	2.02±0.27ab	3.41±0.19a	22.37±0.16b	7.08±0.13b	65.11±0.67a
SNPK	2.23±0.09a	3.46±0.06a	22.76±0.15ab	6.80±0.23b	64.75±0.24a

The Response of Water-Soluble Organic Carbon to Organic Material Applications in Black Soil

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