Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (3): 539-549.doi: 10.3864/j.issn.0578-1752.2019.03.014

• ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Establishment of a Prediction Model of Metabolizable Protein of Concentrate for Mutton Sheep

FU LiXia1,2,MA Tao1,DIAO QiYu1(),CHENG ShuRu2,SONG YaZhe1,SUN ZhuoLin1   

  1. 1 Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081
    2 College of Animal Science and Technology, Gansu Agriculture University, Lanzhou 730070
  • Received:2018-02-12 Accepted:2018-12-29 Online:2019-02-01 Published:2019-02-14

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Abstract:

【Objective】 The aim of this study was to establish a predictive model of metabolizable protein (MP) of concentrates, which is commonly used in feedstuffs of sheep by nutrient or nutrient digestibility. 【Method】 Six 14-month-old Dorper (♂) × thin-tailed Han sheep (♀) sheep with an average body weight of (49.27±3.12) kg fitted with a permanent rumen fistula were selected and used in testing effective degradation rate, rumen undegraded dietary protein (UDP) and intestinal digestibility of UDP by nylon bag method and three-step modified in vitro method. Ten healthy Dorper × thin-tailed Han ram with an average body weight of（47.43±4.41）kg were selected and used in 11 treatment groups including one basic diet group and 10 experimental groups（The Chinese wildrye hay, corn and soybean meal of basic diet were replaced by sorghum, corn, barley, wheat, oat, rapeseed peanut, cottonseed, soybean and DDGS.), respectively. Each period lasted for 20 d (15 d for adaptation and 5 d for trial period). The experimental sheep were prefixed with ear numbers, dewormed with ivermectin, and feed a single column. Due to the differences in the nutrient composition of each group of feeding foods, the amount of feeding in the lowest group was observed and determined during the adaptation as the limit of feeding, fed twice a day, at 8:00 and 16:30, respectively. Feed 600 g, free drinking water. Each ram is a replicate and all rams were used to determine the apparent digestibility of nutrients and microbial synthetic protein (MCP) using the urinary purine derivative method. The MP predictive model was established using proximal analysis of nutrient contents or nutrient digestibility. Using NLIN program in SAS 9.1 to calculate a, b, c values and linear regression and multivariate regression program analysis, MP estimation model was established, and the single factor variance analysis(one-way ANOVA, LSD) was significantly tested. 【Result】 The results showed that the rumen digestibility and digestion rate of CP in different concentrates were different, and CP degradability and intestinal digestibility of UDP of high protein diet were higher. The effective degradation rate of CP rumen in 10 kinds of feed ranged from 43.71% to 60.87%, and intestinal digestibility of UDP ranged from 80.10% to 92.86%. The effective degradation rate of rumen in oat feed was significantly higher than that of other feeds (P＜0.001), while intestinal digestibility of UDP was significantly lower than that of other feeds (P＜0.001). The diets had different composition and apparent digestibility of different nutrients. The digestion and absorption sites of dietary protein did not affect the apparent digestibility of nutrients. The ratio of MP to DP of 10 feeds in this study ranged from 50.96% to 62.33%, The digestible protein (DP, %) prediction model established by dietary CP (%) content was：DP=0.895×CP-2.663 (R 2=0.994, n=10, P＜0.001); The MP (g·kg -1 DM) prediction model established by nutrient contents (%) and apparent nutrient digestibility (%) were: MP=5.323×CP-14.374 (R 2=0.994, n=10, P＜0.001) and MP=5.899×DP+2.077 (R 2=0.984, n=10, P＜0.001).【Conclusion】Digestible protein had a strong relationship with crude protein in diet. The nutrient content and nutrient digestibility in diets were correlated with the presence of MP, and the MP values of concentrate feed could be estimated more accurately by the nutrient and nutrient digestibility in diets.

Key words: mutton sheep, concentrate, microbial synthetic protein, small intestinal digestibility, metabolizable protein, prediction model

"

项目 Items 含量 Content
原料 Ingredients
羊草 Chinese wildrye hay 55.00
玉米 Corn 29.40
豆粕 Soybean meal 14.00
磷酸氢钙 CaHPO4 0.86
食盐 NaCl 0.50
预混料Premix1) 0.24
合计 Total 100
营养水平 Nutrient levels2)
干物质 Dry matter, DM 95.55
有机物 Organic matter, OM 93.68
总能 Gross energy, GE（MJ·kg-1） 16.34
粗蛋白质 Crude protein, CP 11.90
中性洗涤纤维 Neutral detergent fiber, NDF 59.12
酸性洗涤纤维 Acid detergent fibre, ADF 25.22
粗脂肪 Ether digestible organic matter extract, EE 2.21
钙 Ca 0.93
磷 P 0.41

"

项目
Items		 组成 Ingredient
基础
饲粮
Basal diet		 高粱
饲粮
Sorghum
diet		 玉米
饲粮
Corn diet		 大麦
饲粮Barley diet		 小麦
饲粮Wheat diet		 燕麦
饲粮
Oat diet		 菜籽粕
饲粮Rapeseed diet		 花生粕
饲粮
Peanut meal diet		 棉籽粕
饲粮Cottonseed meal diet		 豆粕
饲粮Soybean meal diet		 玉米酒
糟饲粮
DDGS diet
不同精料替换比例 Different concentrate replacement ratio 30.0 30.0 30.0 30.0 30.0 30.0 30.0 30.0 30.0 30.0
玉米corn 19.0 13.2 13.2 13.2 13.2 13.2 13.2 13.2 13.2 13.2 13.2
豆粕Soybean mean 12.2 8.46 8.46 8.46 8.46 8.46 8.46 8.46 8.46 8.46 8.46
羊草Chinese wildrye hay 66.5 46.0 46.0 46.0 46.0 46.0 46.0 46.0 46.0 46.0 46.0
磷酸氢钙CaHPO4 1.41 1.41 1.41 1.41 1.41 1.41 1.41 1.41 1.41 1.41 1.41
石粉Limestone 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15
食盐NaCl 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50
预混料Premix1） 0.24 0.24 0.24 0.24 0.24 0.24 0.24 0.24 0.24 0.24 0.24
合计Total 100 100 100 100 100 100 100 100 100 100 100
营养水平Nutrient levels2）
干物质DM 92.13 90.92 90.79 91.62 91.51 91.58 91.87 91.55 93.96 93.89 91.32
有机物OM 90.15 93.10 93.45 93.77 93.51 94.74 89.06 89.38 92.34 92.07 90.30
总能 GE (MJ·kg-1) 17.92 17.78 17.81 17.75 17.63 17.93 18.25 18.40 18.43 18.21 18.81
粗蛋白质CP 12.03 11.06 10.56 11.50 11.96 12.68 22.12 26.89 20.36 22.55 17.06
中性洗涤纤维NDF 33.20 33.56 33.33 37.84 37.15 33.29 40.13 36.19 41.33 33.55 40.74
酸性洗涤纤维ADF 18.05 17.71 16.82 19.66 19.03 17.43 22.35 19.44 23.67 19.67 19.42
粗脂肪EE 2.70 2.16 2.19 2.01 2.24 2.77 2.04 1.13 1.40 1.65 3.89
灰分 Ash 9.85 6.90 6.55 6.23 6.49 5.26 10.94 10.62 7.66 7.93 9.70
钙 Ca 0.94 1.05 0.94 0.82 0.96 0.96 1.45 1.06 1.03 0.99 0.82
总磷 TP 0.35 0.24 0.29 0.25 0.26 0.29 0.36 0.27 0.32 0.31 0.39

Table 3

Concentrates nutrient levels (DM basis) (%)"

项目
Items		 高粱Sorghum 玉米
Corn		 大麦
Barley		 小麦
Wheat		 燕麦
Oat		 菜籽粕
Rapeseed
meal		 花生粕
Peanut meal		 棉籽粕
Cottonseed
meal		 豆粕Soybean meal 玉米酒糟DDGS
干物质 DM 89.59 89.94 90.60 89.64 90.31 91.60 91.97 92.28 91.74 91.65
有机物 OM 98.27 98.79 95.75 98.33 98.22 93.04 94.39 92.66 92.50 95.19
粗蛋白 CP 10.48 8.88 10.35 13.57 15.15 40.50 58.36 44.99 47.92 30.45
中性洗涤纤维 NDF 14.97 16.98 18.58 14.87 10.25 17.45 12.24 20.17 12.93 23.97
酸性洗涤纤维 ADF 3.01 4.06 8.69 4.66 3.30 13.19 6.65 14.60 7.05 8.66

Table 4

Ruminal degradability of crude protein and intestinal digestibility of undegraded dietary protein of concentrates (%)"

项目
Items		 高粱Sorghum 玉米Corn 大麦
Barley		 小麦
Wheat		 燕麦
Oat		 菜籽粕
Rapeseed
meal		 花生粕
Peanut meal		 棉籽粕
Cottonseed
meal		 豆粕Soybean meal 玉米
酒糟DDGS		 SEM P值
P
value
蛋白质瘤胃降解率 Ruminal degradability of crude protein
6 h 16.64d 24.25bcd 22.73cd 30.30b 44.30a 27.42bc 24.33bcd 29.19b 16.87d 15.77d 1.697 ＜0.01
12 h 20.10d 27.54cd 31.66cd 49.83b 63.53a 35.55c 28.63cd 31.53cd 20.28d 19.73d 2.649 ＜0.01
24 h 24.82c 32.77bc 39.66b 63.25a 72.64a 41.34b 40.82b 42.52b 34.15bc 22.15c 2.948 ＜0.01
36 h 36.18cd 37.66cd 48.51bc 70.28a 80.64a 47.56bc 48.55bc 54.55b 41.45bcd 35.03d 3.302 ＜0.01
48 h 38.77d 47.25cd 59.36bc 74.27a 84.19a 58.01bc 59.51bc 62.18b 54.02bc 38.88d 2.668 ＜0.01
有效降解率Effective degradation rate 45.08e 43.71e 44.38e 49.32de 60.87a 52.04bcd 57.26ab 54.62bcd 56.02abc 51.08cd 1.143 ＜0.01
瘤胃非降解 蛋白质小肠消化率
Small intestine digestibility of undegraded dietary protein		 84.69d 86.23cd 84.23d 84.10d 80.10e 89.25b 92.86a 92.31a 89.26b 87.31bc 0.731 ＜0.01

Table 5

Undegraded dietary protein, microbial synthetic protein and metabolizable protein of concentrates"

项目
Items		 高粱
Sorghum		 玉米
Corn		 大麦
Barley		 小麦
Wheat		 燕麦
Oat		 菜籽粕
Rapeseed
meal		 花生粕
Peanut meal		 棉籽粕
Cottonseed
meal		 豆粕Soybean meal 玉米
酒糟DDGS
瘤胃降解蛋白质 RDP (g·kg-1 DM) 15.59 12.82 15.15 22.16 30.35 68.84 115.23 79.43 85.76 51.16
瘤胃非降解蛋白质 UDP (g·kg-1 DM) 18.98 16.52 18.74 22.31 19.30 63.44 85.85 64.22 67.34 48.89
微生物合成蛋白质 MCP (g·kg-1 DM) 54.50 46.92 50.97 50.64 59.83 73.80 84.74 66.27 81.11 60.87
可代谢蛋白质 MP (g·kg-1 DM) 48.04 41.81 45.46 48.28 50.30 99.47 129.08 96.89 106.18 78.23
可代谢蛋白质占可消化蛋白的比例 The percentage of MP to DP (%) 61.66 55.63 50.96 55.08 53.73 53.33 54.86 62.33 55.14 56.78

Table 6

Apparent nutrient digestibility of different diets (%)"

项目
Items		 高粱
饲粮
Sorghum
diet		 玉米
饲粮Corn diet		 大麦
饲粮Barley diet		 小麦
饲粮
Wheat diet		 燕麦
饲粮
Oat diet		 菜籽粕
饲粮Rapeseed
diet		 花生粕
饲粮Peanut meal diet		 棉籽粕
饲粮Cottonseed meal diet		 豆粕
饲粮Soybean meal diet		 玉米酒糟饲粮
DDGS diet		 SEM P值
P value
干物质消化率
Digestible dry matter (DMD)		 65.29abc 67.40a 64.69bc 65.48abc 63.40c 57.32e 64.20c 59.68d 66.79ab 58.78de 0.41 ＜0.01
有机物消化率
Digestible organic matter (DOM)		 68.65ab 70.44a 68.49ab 68.68ab 66.91b 64.15c 69.97a 61.45d 69.30a 64.47c 0.37 ＜0.01
粗蛋白质消化率
Digestible crude protein (DCP)		 64.03e 64.60de 71.05b 67.09cd 67.63c 77.47a 79.73a 71.75b 80.16a 73.76b 0.65 ＜0.01
中性洗涤纤维消化率
Digestible neutral detergent fiber (DNDF)		 42.96bc 42.56bcd 47.59ab 48.79a 38.86cde 37.09de 43.71abc 36.01e 42.66bcd 41.67cd 0.67 ＜0.01
酸性洗涤纤维消化率
Digestible acid detergent fiber (DADF)		 42.91ab 37.38c 45.10a 45.85a 38.46bc 34.35dc 46.96a 30.91d 47.40a 35.91c 0.76 ＜0.01

Table 7

Prediction model for metabolizable protein using chemical composition and apparent digestibility"

递推回归模型Recursive regression equation 决定系数 R2 P值 P value
MP=5.323CP-14.374 0.994 ＜0.001
MP=5.268CP+0.532DM-62.319 0.995 ＜0.001
MP=5.290CP+0.669DM-0.173ADF-71.664 0.995 ＜0.001
MP=5.318CP+1.262DM-0.877ADF+0.376NDF-126.679 0.995 ＜0.001
MP=5.373CP+1.481DM-0.827ADF+0.404NDF+0.254OM-174.198 0.995 ＜0.001
MP=5.899DP+2.077 0.984 ＜0.001
MP=5.710DP-0.530DOM-37.165 0.986 ＜0.001
MP=5.500DP-1.741DOM+1.371DDM+38.005 0.989 ＜0.001
MP=5.678DP-1.550DOM+1.344DDM-1.129DNDF+32.093 0.990 ＜0.001
MP=5.791DP-1.587DOM+1.552DDM-1.871DNDF+0.443DADF+19.832 0.990 ＜0.001
DP=0.895CP-2.663 0.994 ＜0.001
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