黄瓜白粉病抗性基因定位及候选基因分析

doi:10.3864/j.issn.0578-1752.2018.17.017

Abstract

Abstract: 【Objective】Identification and fine mapping of the candidate region and the gene associated with powdery mildew resistance is significant for gene cloning and functional genomics research in cucumber breeding. 【Method】Powdery mildew resistant cucumber line was identified using single capsule inoculation. Bulked segregant analysis sequencing (BSA-seq) technology was intended to complete the primary mapping of the PMR gene. 【Result】 Resistance identification showed inbred lines 74 exhibited excellent resistance to powdery mildew, in contrast, the inbred lines 80 showed high susceptibility. These two genotypes were used to construct F₁ segregation population which was then extended to F₂ population for an inheritance study. Our results indicated the resistance gene from inbred line 74 was partial-recessive inherited. BSA-seq localized the major resistance gene, named PM⁷⁴, to a 15-25 Mb genomic region in cucumber on chromosome 5. The resistance gene was further located with a 23 844 bp physical distance between SSR15321 and SSR07531 at the genetic distance of 3.06 cM and could explain 41.95% phenotypic variation. 17 annotated genes were found within the predicted candidate region, including one gene belongs to TIR-NBS-LRR gene family, named Cucsa.275630. 【Conclusion】This study mapped a PMR gene within the 238 kb physical interval on Chromosome 5, a TIR-NBS-LRR type gene was identified and would be focused in the future study.

Key words: cucumber, powdery mildew, Bulked Segregant Analysis (BSA), NBS gene, molecular marker

HAO JunJie, LI Lei, WANG Bo, QIN YuHong, CUI Jian, WANG Ying, WANG PeiSheng, JIANG ZhiXun, SUN JiLu, WANG ZhenQing, YUE Huan, ZHANG ShouCai . Fine Mapping and Analysis Candidate Gene to Powdery Mildew in Cucumber (Cucumis sativus L.)[J].Scientia Agricultura Sinica, 2018, 51(17): 3427-3434.

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