Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (13): 2561-2569.doi: 10.3864/j.issn.0578-1752.2018.13.011

• HORTICULTURE • Previous Articles     Next Articles

Responses of Leaf Assimilate Export To Lowlight Stress in Melon

YANG LiuYan, CHEN JingJing, CHEN NianLai   

  1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070
  • Received:2017-12-14 Online:2018-07-01 Published:2018-07-01

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Abstract: 【Objective】The objective of this study is to evaluate the responses of melon leaf photosynthesis, sucrose synthesis and stachyose loading to low light stress during fruit development stage, and the difference in assimilate export between lowlight tolerance and sensitive cultivars, to support the further analysis of sugar unloading and accumulation in fruits of the lowlight tolerant cultivars. 【Method】 A low light-tolerant cultivar (Yujinxiang) and a low light-sensitive cultivar (Yuxuesanhao) of melon (Cucumis melo L.) were used as materials. The melon plants were cultured in greenhouse and were shaded after pollination. And then the mature leaves from the fruit nodes were collected every five days for measurement. The leaf chlorophyll content, gas exchange parameters, levels of soluble sugars (fructose, glucose, sucrose, galactinol, raffinose, stachyose) and starch, activities of sucrose phosphate synthase (SPS), sucrose synthase (SS), acid invertase (AI), neutral invertase (NI), galactinol synthase (GS) and stachyose synthase (STS) were determined. 【Result】 Under shading condition, leaf chlorophyll a/b, net photosynthetic rate (Pn), and contents of glucose, fructose and sucrose in the leaves of both cultivars decreased, but the starch level increased. The reduction of chlorophyll a/b in Yujinxiang leaves (10.0%) was larger than that in Yuxuesanhao leaves (5.8%), but the reduction of leaf Pn, sucrose content and increment of starch content were less in Yujinxiang (30.3%, 30.9% and 3.6%, respectively) than those of Yuxuesanhao (45.2%, 60.6% and 20.4%). Activities of sucrose metabolism enzymes (SPS, SS, AI and NI) decreased after shading, and the reduction of SPS and SS activities were significantly less in Yujinxiang (16.5% and 30.0%) than in Yuxuesanhao (31.6% and 40.5%), but the reduction of AI and NI activities was larger in Yujinxiang (23.8% and 12.7%) than in Yuxuesanhao (18.3% and 1.8%). Galactinol level and GS activity decreased after shading, but no significant genotypic difference was observed. Contents of raffinose and stachyose as well as the activity of STS all decreased after shading. The reduction of raffinose content was larger, the reduction of stachyose content and STS activity was less in Yujinxiang (65.3%, 79.5% and 23.8%, respectively) than those in Yuxuesanhao (35.0%, 83.3% and 32.4%). 【Conclusion】The reduction of leaf sucrose synthesis and stachyose loading after shading was less in Yujinxiang than in Yuxuesanhao, which indicates that the low light tolerant cultivar has stronger leaf assimilate export capacity than the sensitive cultivar under shading. 

Key words: melon, shading, gas exchange parameters, sucrose synthesis, stachyose loading, assimilates export

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