水稻根际和非根际土磷酸酶活性对碳、磷添加的响应

doi:10.3864/j.issn.0578-1752.2018.09.004

Abstract

Abstract: 【Objective】 Therefore, the objective of this experiment was to study the effects of exogenous nutrient input on soil microbial activity and phosphatase activity which was closely related to the phosphorus cycle in paddy soil, so as to clarify the responses of the phosphorus activity to carbon and phosphorus additions in rice rhizosphere and bulk soils. It is of great significance to guide the fertilization and management of rice soil and to realize the sustainable utilization of farmland. 【Method】 The typical phosphorus-limited rice soil in Hunan was selected for pot experiment. The treatments included: no carbon and phosphorus additions (CK), carbon addition (C), phosphorus addition (P), and carbon and phosphorus additions (CP). Acid phosphatases (ACP) and alkaline phosphatases (ALP) of the rhizosphere and bulk soils were determined by 96-microtiter plateau method. 4 soil phosphorus (CaCl₂-P, Citrate-P, Enzyme-P and HCl-P) were measured by biologically based phosphor (BBP) method to investigate the response of carbon and phosphorus addition and 4 soil phosphorus components based on the biological availability to soil enzyme activity.【Result】Compared with CK, the above-ground biomass of C, P and CP significantly increased by 29.76%, 84.03% and 87.94%, respectively (P＜0.05), and the underground biomass significantly decreased by 20.13% and increased by 57.49% and 56.53%, respectively (P＜0.05), which was consistent with the content of plant total phosphor (P＜0.05). The results showed that pH, the contents of NH₄⁺-N and Olsen-P in rhizosphere soil were significantly lower than those in bulk soil (P＜0.05). The content of microbial biomass phosphorus (MBP) in rhizosphere soil (except CP treatment) was significantly higher than that in bulk soil (P＜0.05). Carbon and phosphorus additions had a significant regulatory effect on 4 soil phosphorus components based on the biological availability in paddy rhizosphere and bulk soils (P＜0.05). Olsen-P and MBP had a significant negative correlation with ALP (P＜0.05), but no significant correlation with ACP, indicating it was obvious for microbes to utilize available nutrients. Phosphatase activity in bulk soil was mainly affected by Olsen-P and MBP, as well as CaCl₂-P and Citrate-P content, while soil water content, pH, NH₄⁺-N and root biomass, as well as HCl-P and Enzyme-P contents mainly affected phosphatase activity in rhizosphere soil. 【Conclusion】 The application of P and CP addition could improve the microbial activity of phosphorus-limited rice soil, increase the biomass significantly, and improve the rhizosphere microbes benefit and amendment the soil environment as well as benefit the health of paddy field ecosystem.

Key words: phosphatases activity, rhizosphere soil, BBP method, phosphorus cycle, phosphorus-limited rice soil, biomass

LIU YuHuai, WEI XiaoMeng, WEI Liang, ZHU ZhenKe, GE TiDa, ZHANG YanJie, LU ShunBao, WU JinShui. Responses of Extracellular Enzymes to Carbon and Phosphorus Additions in Rice Rhizosphere and Bulk Soil[J].Scientia Agricultura Sinica, 2018, 51(9): 1653-1663.

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Responses of Extracellular Enzymes to Carbon and Phosphorus Additions in Rice Rhizosphere and Bulk Soil

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