小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响

doi:10.3864/j.issn.0578-1752.2017.08.002

Abstract

Abstract: 【Objective】Lipoxygenase (Lox) is closely related to flour color. The objective of this study was to investigate the influence of different allelic variations at TaLox-B loci on Lox activity and flour color in wheat to provide a theoretical basis for improving flour color and related quality traits in breeding programs.【Method】A total of 122 wheat cultivars and advanced lines from Henan province were used to measure the Lox activities and flour color by spectrophotometer and colorimeter. The functional markers Lox16, Lox18 and Lox-B23 were used to identify the polymorphism of the Lox genes on chromosome 4BS in current Henan wheat cultivars and advanced lines.【Result】There were significant differences of Lox activities, L^*, a^*and b^*values as well as Wht data among different cultivars. Genotyping results indicated two alleles TaLox-B1a and TaLox-B1b at TaLox-B1 locus in these wheat varieties, with 63.9% and 39.1%, respectively. At TaLox-B2 locus, two alleles TaLox-B2a and TaLox-B2b were identified by the functional marker Lox-B23, with 57.4% and 42.6%, respectively. It was also found two alleles TaLox-B3a and TaLox-B3b at the TaLox-B3 locus, with 41.8% and 48.2%, respectively. Six allele combinations of Lox genes, i.e., TaLox-B1a/TaLox-B2a/TaLox-B3a, TaLox-B1a/TaLox-B2a/TaLox-B3b, TaLox-B1a/TaLox-B2b/TaLox-B3b, TaLox-B1b/TaLox-B2a/TaLox-B3a, TaLox-B1b/TaLox-B2a/TaLox-B3b and TaLox-B1b/TaLox-B2b/TaLox-B3b were found with 41.8%, 15.6%, 6.6%, 28.7%, 5.7% and 1.6%, respectively. Analysis of association of Lox alleles at different TaLox-B loci and Lox activities and flour color parameters indicated that the allelic variation at a single locus showed diverse effects on Lox activities and flour color. For a^*value, the allelic effect was not significant. For Lox activities, the order of effect was TaLox-B2a > TaLox-B2b (P＜0.05), TaLox-B3a > TaLox-B3b (P＜0.01). The Wht value of TaLox-B2a was significantly lower than that of TaLox-B2b (P＜0.01), and TaLox-B3a showed also a significant lower Wht value than TaLox-B3b (P＜0.05). These results suggest that TaLox-B2 and TaLox-B3 significantly affect Lox activities and flour color. Further analysis found that the genotype TaLox-B1a/TaLox-B2a/TaLox-B3a had the highest Lox activity and b^* and the lowest a^* and Wht value, and TaLox-B1b/TaLox-B2b/TaLox-B3b had the lowest Lox activity and b^* and the highest L^*, a^* and b^* values. 【Conclusion】Among the 6 different combinations of three Lox genes in Henan wheat varieties, the genotype TaLox-B1a/TaLox-B2a/TaLox-B3a possess the highest Lox activity and the lowest Wht value (P＜0.05), and wheat cultivars with TaLox-B1b/TaLox-B2b/TaLox-B3b have the lowest Lox activity and the highest Wht value (P＜0.05). Therefore, the genetic control of Lox activities plays a key role in the improvement of wheat quality.

Key words: common wheat, lipoxygenase gene, functional marker, flour color, Henan

ZHANG FuYan, CHEN Feng, CHENG ZhongJie, YANG BaoAn, FAN JiaLin, CHEN XiaoJie, ZHANG JianWei, CHEN YunTang, CUI Long. Effects of TaLox-B Alleles on Lipoxygenase Activity and Flour Color in Wheats[J].Scientia Agricultura Sinica, 2017, 50(8): 1370-1377.

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Effects of TaLox-B Alleles on Lipoxygenase Activity and Flour Color in Wheats

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