Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (24): 4824-4832.doi: 10.3864/j.issn.0578-1752.2016.24.014

• RESEARCH NOTES • Previous Articles    

Effect of Chemical Hybridization Agent SQ-1 and Arabinogalactan Proteins on the Embryos Obtaining in Wheat Intervarietal and Wild Crosses

WANG Kun-yang1, ZHANG Wei1, ZHANG Shuang-xi2, LIU Hong-wei1, WANG Ke1, DU Li-pu1, LIN Zhi-shan1, YE Xing-guo1   

  1. 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Genetic Improvement, Beijing 100081
    2Crop Research Institute, Ningxia Academy of Agricultural-Forestry Sciences, Yinchuan 750105
  • Received:2016-06-23 Online:2016-12-16 Published:2016-12-16

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Abstract: 【Objective】The aims of this study are to clarify the effect of SQ-1 on the embryo formation in the hybridization among wheat varieties and between wheat and its relative or wild species as well as the effect of AGP on haploid embryo production and haploid plant production in the cross of wheat and maize. The findings in this study will be potentially useful to the efficiently conduction of wheat hybridization breeding and haploid breeding.【Method】In this study, the effectiveness of SQ-1 on the seed-setting after wheat varieties are crossed with other wheat varieties, rye, and maize, and AGP on haploid wheat induction in the cross between wheat and maize were investigated by spraying wheat plants with SQ-1 at booting stage, pollinating the sterile spikes with wheat, rye or maize pollens at flowering period, and treating the wheat spikes pollinated maize pollens with an AGP containing solution, respectively. In addition, in vitro culture was used to rescue the haploid wheat embryos from wheat and maize crossing. Florets pollinated, caryopses formed, grains obtained, embryos cultured, embryos germinated, and plantlets obtained were counted to calculate, caryopses formation rate, setting rate, haploid embryos induction rate, haploid embryos induction rate, and haploid production rate. All the data were analyzed for significant difference evaluation. 【Result】It was found that seed setting rate for the crossing among wheat varieties was 19.8%-83.3% from SQ-1 treatment, and 69.4%-93.0% from artificial emasculation treatment. But, different wheat varieties as female parent showed different responses to SQ-1, and Fielder displayed the most sensitivity to SQ-1. For the wild crossing between a wheat line Chinese Spring (CS) and a rye line Lanzhou Heimai, the seed setting rate was 65.5% from SQ-1 treatment, and 78.8% from artificial emasculation method. All the F1 hybrids from the two treatments had 28 chromosomes. For the wild crossing between wheat and maize, wheat haploid embryo induction rate was 1.11%-1.41% from SQ-1 treatment, and 2.38%-14.29% from artificial emasculation method. Compared with the control treatment, application of 0.5-2.0 g·L-1 AGP in the treating solution assisted the wheat haploid embryo production rate to be improved to 17.0% from 11.5%. Besides, in the wheat haploid embryos induced by maize pollen, it was found that 13.07% of the embryos developed 2-6 seedlings. Under a microscope, more than one original embryonic axis were observed on the haploid wheat embryos induced by maize pollen when they were rescued by in vitro culture on medium. Cytological examination revealed that the in vitro culture wheat plants had 21 chromosomes. 【Conclusion】The above results indicated that SQ-1 application reduced not only seed setting in the cross among different wheat varieties and between wheat and rye, but also haploid wheat embryo induction rate in the cross between wheat and maize. AGP application improved haploid wheat embryo induction, germination, and plantlets obtaining rates between the hybridization of wheat and maize.

Key words: common wheat, chemical hybridization agent (CHA)SQ-1, arabinogalactan proteins (AGP), hybridization among wheat varieties, wide crosses, haploid embryos

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