不同番茄品种对番茄黄化曲叶病毒的抗病性分析

doi:10.3864/j.issn.0578-1752.2016.14.020

Abstract

Abstract: 【Objective】Tomato yellow leaf curl virus (TYLCV) disease is a serious threat to tomato production worldwide, breeding resistant varieties is the most economical, effective and friendly way to control this disease. Most of the tomato varieties resistant to TYLCV used in production of China were introduced from abroad. Many new tomato varieties resistant to TYLCV have been obtained by breeding, but the resistance is unknown. In this paper, the resistance levels of the new tomato varieties resistant to TYLCV bred by Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences were assessed and the expression of resistance genes Ty-1/Ty-3a and the pathogenesis-related proteins glucanase/chitinase were analyzed. The purpose is to provide a reference for rational utilization of germplasm resources and reduce the harm of TYLCV.【Method】Four tomato varieties Qiuguang 285, Peng 137×246, Qiuguang 120 and Qiuguang 81 were used as the experimental materials. TYLCV disease incidence was observed in the field. The disease resistance of these tomato varieties was examined by investigating the disease incidence and index. The resistance levels of the new tomato varieties resistant to TYLCV were assessed and the expression of resistance genes and the pathogenesis-related proteins were analyzed by RT-PCR. 【Result】 The resistance of the hybrid was no obvious differences with that of the pure and the resistance of the two genes was a little higher than that of single resistance genes, but yet no significant differences. Semi-quantitative RT-PCR results showed that the expression of genes Ty-3a and Ty-1 increased with the increase of the incidence of TYLCCV in different tomato varieties. The expression level of Ty-3a in four tomato varieties from weak to strong is Qiuguang 285, Peng 137×246, Qiuguang 120 and Qiuguang 81. The expressionof Ty-1 in Qiuguang 81 was obviously higher than that of in Qiuguang 120. The gene expression of glucanase and chitinase in vivo was significantly higher than that in the control. The expression of the two genes increased with the increase of the time. In the 20 and 30 days after appearing of disease, the expression of glucanase gene in Qiuguang 81 with the Ty-1+Ty-3a was significantly higher than that in other varieties.【Conclusion】The resistance of four tomato varieties to TYLCV from weak to strong is Qiuguang 285, Peng 137×246, Qiuguang 120 and Qiuguang 81. After the infection of TYLCV, the expression levels of resistance genes increased with the time of disease in different tomato varieties. The expression levels of glucanase and chitinase genes in vivo were significantly higher than those in the control. The results suggested that the high resistance of variety to TYLCV is related to the high expression of resistance gene and pathogenesis-related proteins.

Key words: Tomato yellow leaf curl virus, resistance gene, pathogenesis-related proteins, semi-quantitative RT-PCR

TIAN Zhao-feng, LIU Wei-cheng, HOU Ling-yu, XIE Hua, LUO Chen, CHAI Min. Analysis of Resistance of Different Tomato Varieties to Tomato yellow leaf curl virus in Tomato[J].Scientia Agricultura Sinica, 2016, 49(14): 2850-2856.

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Analysis of Resistance of Different Tomato Varieties to Tomato yellow leaf curl virus in Tomato

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