西双版纳黄瓜多心室性状的QTL定位

doi:10.3864/j.issn.0578-1752.2015.16.011

Abstract

Abstract: 【Objective】Xishuangbanna cucumber (Cucumis sativus L. var. xishuangbannanesis Qi et Yuan) is an elite Chinese original cucumber germplasm that produces fruits with multi-locule-number, of which the diameter is much larger than the 3-locules fruit. The purpose of this study is to investigate the inheritance and QTL locations of the multi-locule-number trait in Xishuangbanna cucumber. The results of the study will provide a preliminary basis for further study on mechanism of multi-locule-number trait and molecular assistant selection breeding, they also can provide helpful information for improvement of fruit characters of cucumber.【Method】The ovaries of Beijingjietou cucumber (CC3, 3-locules) and Xishuangbanna cucumber (SWCC8, 5-locules) were harvested at different developmental stages for paraffin sections. According to the genetic linkage map constructed based on 124 F₉ recombinant inbred lines (RILs) derived from the cross of Beijingjietou×Xishuangbanna, the multi-locule-number QTLs were analyzed with the locule number statistics of RILs population in two seasons. 【Result】 Histological observation showed that the ovary of Xishuangbanna cucumber is consisted with five involute carpels which formed the multi-locules. By comparing with 3-locules cucumber ovary, the 5-locules cucumber ovary has a larger diameter and more placentas. QTL analysis indicated that 5 QTLs (LOD＞2.5) distributed on chromosomes 1, 2, and 6. The major QTL ln1.1 locating between SSR16472-SSR12070 was detected in two seasons, having two LOD scores of 10.96, 9.24 and R² of 39.24%, 20.49%, respectively. The another major QTL ln1.3 locating between UW083751-SSR04278 was detected in two seasons, having two LOD scores of 27.31 and 21.45, and R² of 75.90% and 47.11%, respectively. The rest QTLs were minor QTLs. Combined with previous findings, there were two genes that control multi-locule-number in ln1.1 and ln1.3 regions, respectively. The two genes in ln1.1 region were WD40 repeat protein gene, Csa1M071910.1 and Csa1M072490.1. The two genes in ln1.3 region were Aux/IAA auxin responsive genes, Csa1M231530.1 and Csa1M207820.1.【Conclusion】 The QTLs of ln1.1 and ln1.3 located on Chr.1 were the major QTLs that control the multi-locule-number trait. The Aux/IAA auxin responsive genes, Csa1M071910.1 and Csa1M072490.1, and the two WD40 repeat protein genes, Csa1M231530.1 and Csa1M207820.1, were predicted as candidate genes.

Key words: Xishuangbanna cucumber, multi-locule-number, QTL mapping, paraffin section

ZHANG Kai-jing, SONG Hui, BO Kai-liang, LI Ji, MA Zheng, LOU Qun-feng, QIAN Chun-tao, CHEN Jin-feng. QTL Mapping of Multi-Locule-Number Trait in Xishuangbanna Cucumber[J].Scientia Agricultura Sinica, 2015, 48(16): 3211-3220.

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