小麦生理型雄性不育系微丝骨架和胼胝质的变化与其相关基因的表达分析

doi:10.3864/j.issn.0578-1752.2015.14.001

Abstract

Abstract: 【Objective】The organization of actin filaments and callose and the expression of related genes in physiological male sterility wheat pollen cells were studied to reveal the relationship between actin filaments and callose and pollen abortion in physiological male sterility wheat. The results of the study will provide a theoretical basis for further study on the mechanism of male sterility induced by chemical hybridizing agents SQ-1 in wheat. 【Method】The physiological male sterility line ms(A)-Xinong1376 and corresponding normal fertile wheat (A)-xinong1376 were used as test materials. The TRITC-phalloidin was used to stain actin filaments and Aniline blue was used to stain callose. QRT-PCR was used to analyze the expression of TaADF (Actin depolymerizing factor) and TaGSL (Glucan synthase-like).【Result】At prophase I, metaphase I, and anaphase I, there were no significant differences between physiological male sterility and male fertility. At prophase I, actin filaments distributed in cytoplasm of the cell and there was also distribution of some actin filaments in nuclear zone. At metaphase I, actin filaments distributed in cytoplasm. They were stained deeper in the spindle position and formed actin spindle. At anaphase I, actin filaments between the poles of the chromosomes were stained deeper, so there were more actin filaments distributed there. At early telophase I, we observed that there were no sharp actin filaments at leading edges of phragmoplasts and the overlapped actin filaments at midline were obscure in physiological male sterility. At late telophase I, there was a cell plate at the midline of phragmoplasts, it was linear and smooth, but in the physiological male sterile line, the linear cell plate was not seen, the midzone of dyads was hollow. At the same time, the deposition of callose on the cell plate was insufficient and the cell plate was wrinkled and cleft. At tetrad, actin filaments were obscure and had no silky feeling and callose fluorescence was weaker in the physiological male sterile. Then qRT-PCR technique was performed to analyze the expression patterns of the TaADF gene and TaGSL gene in sterile and fertile anthers during meiosis. The results indicated that the transcript of TaADF in the treated anthers was 4.28 times higher than that of the control. It indicated that because of the increased expression of TaADF, the rate of dissociation of actin filaments was increased, so the microfilaments were obscure. The transcript of TaGSL in the treated anthers was 0.83 times lower than that of the control. Callose deposition was influenced by SQ-1. 【Conclusion】The increased expression level of TaADF in the physiological male sterility line destroyed the normal structure of actin filaments. The actin filaments could not execute their functions. Then it may influence certain metabolic pathways associated with fertility in anther development. At the same time, abnormal actin filaments caused abnormal cell plate formation which may have an effect on callose deposition. Therefore, it was estimated that it has a close relationship among the changes of actin filaments and callose and the physiological male sterility induced by SQ-1.

Key words: common wheat, physiological male sterile, meiosis, actin filaments, callose

ZHANG Jiao, ZHU Qi-di, JU Lan, ZHANG Gai-sheng, YU Yong-ang, NIU Na, WANG Jun-wei, MA Shou-cai. Organization Changes of Actin Cytoskeleton and Callose with the Expression Analysis of Related Genes in Physiological Male Sterile Wheat[J].Scientia Agricultura Sinica, 2015, 48(14): 2687-2696.

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Organization Changes of Actin Cytoskeleton and Callose with the Expression Analysis of Related Genes in Physiological Male Sterile Wheat

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