Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (13): 2613-2624.doi: 10.3864/j.issn.0578-1752.2014.13.013

• HORTICULTURE • Previous Articles     Next Articles

Isolation of Florigen Gene PdFT and Its Effects on Flowering of Tree Peony（Paeonia delavayi Franch.）

 ZHU  Fu-Yong, LIU  Chuan-Jiao, XUE  Jing-Qi, WANG  Shun-Li, ZHANG  Ping, REN  Xiu-Xia, ZHANG  Xiu-Xin   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081
  • Received:2014-02-21 Online:2014-07-01 Published:2014-04-16

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Abstract: 【Objective】The objective of this study was to clone the homolog of FT gene from tree peony and analyze the expression pattern and the potential function of flowering locus T (FT) of flowering in Paeonia delavayi Franch..【Method】The homologous FT gene, PdFT, was cloned by reverse transcript polymerase chain reaction (RT-PCR). The expression patterns of PdFT in different organs, developmental stages of flowering and treatments of P. delavayi Franch. were analyzed by using qRT-PCR and the relationship between the PdFT expression level and the different states of flower bud development was also studied. At the same time, PdFT was inserted into vector pET-28a and expressed in E. coli BL21 (DE3). 【Result】The full length of open reading frame (ORF) of PdFT was 522 bp, encoding 173 amino acid residues. The GenBank accession number is KF113360. Alignment of the AA sequences revealed that PdFT contains a conserved domain, which possesses the characteristics of the PEBP family. The result also suggested that PdFT shares high identity with FT proteins from other plants. qRT-PCR analysis of PdFT expression pattern in different organs showed that the highest expression levels were appeared in the buds of P. delavayi, followed by roots, stems and leaves. The expression pattern of PdFT from bud swelling to big-like flower-bud showed a decreased trend. The expression level of PdFT under long day condition was higher than that under short day condition. Cool treatment could decrease PdFT expression in roots and stems. The expression of PdFT under different floral conditions showed difference. The expression of PdFT in abortive flower buds was lower than that in normal bud. GA3 treatment and removing leaves could increase the expression of PdFT. The results of SDS-PAGE showed that the expressed protein in E. coli BL21 (DE3) was consistent with the size of expected. 【Conclusion】The PdFT isolated from P. delavayi Franch. is highly homologous with the other FT genes isolated from other species and belongs to FT subfamily. The higher expression of PdFT in buds suggested that it may play a role in regulating flowering in tree peony. PdFT gene cloning and expression analysis will lay a genetic basis for investigating the molecular mechanism of flowering in P. delavayi Franch.

Key words: Paeonia delavayi Franch. , FLOWERING LOCUS T , qRT-PCR , prokaryotic expression

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