Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (10): 2062-2068.doi: 10.3864/j.issn.0578-1752.2014.10.020

• RESEARCH NOTES • Previous Articles    

Morphologic and Physiologic Characterization of an Ovule Abortion Mutant in Brassica napus

 XU  Wang-Jie, FU  Ying, DONG  Hong-Li, CHEN  Zhi-Fu, ZHANG  Qin-Yun, MAO  Shao-Shuai, HE  Ya-Jun, QIAN  Wei   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400716
  • Received:2013-12-22 Online:2014-05-20 Published:2014-03-03

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Abstract: 【Objective】The development of ovule is closely related with seed-setting and seed weight, which are components of seed yield in rapeseed. The investigation of morphologic and physiologic characterization of abortion mutant will benefit understanding the mechanism of development of ovule. 【Method】 In this study, OVA047，an ovule abortion mutant, was characterized in histological anatomy and investigated for the effects of nutrition of boron and temperature in Chongqing. Acetic red dyeing method was used to identify pollen fertility of OVA047. The buds of OVA047 which were about to open were selected for artificial emasculation and pollination. The ovary samples were taken and the pollen tube elongation was observed every 4 hours after artificial pollination. Foliar boron treatments were applied for one month seedlings of the normal materials and OVA047. Vegetative growth, pollen fertility and seed setting rate were investigated. Buds were artificially stripped, emasculated and pollinated, pollination time was tagged, statistical analysis was made for abortive rate in different periods after pollination. Daily average temperature and daily maximum temperature were recorded and the correlation between temperature and abortive rate was investigated. The data obtained were analyzed by using software SAS.【Result】 Normal sexual organisms and pollination could be detected and the pollen dye ability rate was 95.90%。Pollen tube could germinate and reach the ovary. The development of ovule was found in good by anatomy on 7 day and 14th day after pollination, but most of ovules stopped growth 20 days after pollination in OVA047, especially at the top of inflorescence with 0.8 seeds per pod 35 days after pollination, 4% of the control. The content of boron in leaf was increased with the content of foliar-applied boron treatment in both normal material and OVA047. No significant differences were detected between OVA047 and control in the content of boron in leaf in the same treatment of boron fertilization. However, in different treatments of boron fertilization, all the OVA047 still exhibited low seed-setting (3.2) and around 60% of ovule abortion ratio in the inflorescence while the control still exhibited normal seed-setting (29). Two years observation results showed that large-scale ovule abortion phenomenon at the top, middle and basal of the inflorescence occurred in the significant temperature rise period when OVA047 grown in Chongqing, the later ovule abortion was increased with the daily temperature. A strong and positive association was found between ovule abortive rate of OVA047 and daily maximum temperature.【Conclusion】The ovule abortion of OVA047 is not due to fertilization failure and possible does not relate with boron fertilization, but associate with temperature.

Key words: Brassica napus , ovule abortion , boron , temperature

[1]王汉中. 中国油料产业发展的现状、问题与对策. 中国油料作物学报, 2005, 27(4): l00-l05.

Wang H Z. Development status, problems and countermeasures of Chinese oil industry. Chinese Journal of Oil Crop Sciences, 2005, 27(4): 100-105. (in Chinese)

[2]傅廷栋. 中国油菜生产与品种改良. 华中农业大学学报, 1999, 18(6): 501-504.

Fu T D. Rape production and variety improvement in China. Journal of Huazhong Agricultural University, 1999, 18(6): 501-504. (in Chinese)

[3]贺佳玉, 李云, 姜金仲, 曹春伟. 植物胚败育机理及其离体培养挽救技术之研究进展. 中国农学通报, 2008, 24(1): 141-146.

He J Y, Li Y, Jiang J Z, Cao C W. Research progress of plant embryo abortion mechanism and its saving by culture in vitro. Chinese Agricultural Science Bulletin, 2008, 24(1): 141-146. (in Chinese)

[4]石磊, 徐芳森. 植物硼营养研究的重要进展与展望. 植物学通报, 2007, 24(6): 789-798.

Shi L, Xu F S. Main progresses in boron nutrition research in plants and its prospects. Chinese Bulletin of Botany, 2007, 24(6): 789-798. (in Chinese)

[5]杨淑娟, 毛桂莲, 孙驰. 钙和硼对芦荟花粉萌发和花粉管生长的影响. 安徽农业科学, 2010, 38(14): 7238-7239, 7282.

Yang S J, Mao G L, Sun C. Effects of Calcium and Boron on pollen germination and pollen tube growth of Aloe vera L.var.Chinesis. Journal of Anhui Agricultural Sciences, 2010, 38(14): 7238-7239, 7282. (in Chinese)

[6]邹小云, 陈伦林, 李书宇, 邹晓芬, 张建模, 宋来强. 氮、磷、钾、硼肥施用对甘蓝型杂交油菜产量及经济效益的影响. 中国农业科学, 2011, 44(5): 917-924.

Zou X Y, Chen L L, Li S Y, Zou X F, Zhang J M, Song L Q. Effect of nitrogen, phosphorus, potassium, and boron fertilizers on yield and profit of hybrid rapeseed (Brassica napus L.). Scientia Agricultura Sinica, 2011, 44(5): 917-924. (in Chinese)

[7]邹长松, 余迪求. 植物有性生殖对温度胁迫反应的研究进展. 云南植物研究, 2010, 32(6): 508-518.

Zou C S, Yu D Q. Temperature stress on plant sexual reproduction. Acta Botanica Yunnanica, 2010, 32(6): 508-518. (in Chinese)

[8]Elster R, Bommert P, Sheridan W F, Werr W. A nalysis of four embryo-specific mutants in Zea mays reveals that incomplete radial organization of the proembryo interferes with subsequent development. Development Genes and Evolution, 2000, 210: 300-310.

[9]Gehring M, Choi Y, Fischer R L. Imprinting and seed Development. The Plant Cell, 2006, 16: S203-S213.

[10]李俊, 罗莉霞, 王转, 李均, 陈坤荣, 任莉, 方小平. 人工合成具有白菜或甘蓝细胞质的甘蓝型油菜. 作物学报, 2010, 36(8): 1280-1285.

Li J, Luo L X, Wang Z, Li J, Chen K R, Ren L, Fang X P. Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa cytoplasm, Acta  Agronomica Sinica, 2010, 36(8): 1280-1285. (in Chinese)

[11]Hiratsuka S, Zhang S L, Nakagawa E, Kawai Y. Selective inhibition of the growth or incompatible pollen tubes by S-protein in the Japanese pear. Sex Plant Reprod, 2000, 13: 209.

[12]刘善江, 赵丽萍. 植株中全硼测定方法的研究. 华北农学报, 2007, 22(2): 169-170.

Liu S J, Zhao L P. Study on testing method of total boron content in plant. Acta Agriculturae Boreali-Sinica, 2007, 22(2): 169-170. (in Chinese)

[13]Hsu S F, Lai H C, Jinn T L. Cytosol-localized heat shock factor- binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis. Plant Physiology, 2010, 153(2): 773-784.

[14]Hsu S F, Jinn L. AtHSBP functions in seed development and the motif is required for subcellular localization and interaction with AtHSFs. Plant Signaling & Behavior, 2010, 5(8): 1042-1044.

[15]陈佳颖, 赵剑, 刘晓, 李超, 林冬枝, 董彦君, 叶胜海, 张小明. 一个新水稻温敏感叶色突变体的遗传分析及其基因分子定位. 植物学报, 2010, 45(4): 419-425.

Chen J Y, Zhao J, Liu X, Li C, Lin D Z, Dong Y J, Ye S H, Zhang X M. Genetic analysis and molecular mapping of a new thermo-sensitive leaf-color mutant in Oryza sativa. Chinese Bulletin of Botany, 2010, 45(4): 419-425. (in Chinese)

[16]祁鲁, 刘晓, 陈佳颖, 林冬枝, 董彦君, 徐建龙. 一个新的水稻温敏感叶色突变体基因定位分析. 核农学报, 2010, 24(2): 220-224.

Qi L, Liu X, Chen J Y, Lin D Z, Dong Y J, Xu J L, Molecular mapping analysis of a new thermo-sensitive leaf-color mutant in Oryza sativa. Journal of Nuclear Agricultural Sciences, 2010, 24(2): 220-224. (in Chinese)

[17]Leopold A C, Nooden L O. Hormonal regulatory system in plants//Scott T K. Hormonal Regulation of Development II. Berlin: Springer-Verlag, 1984: 10-11.

[18]Franklin P G, Pearce R B, Roger L M. Physiology of Crop Plants. Ames, Iowa: Iowa State University Press, 1985: 201-242.

[19]武斌, 李宗芸, 杨素春, 张迪. 几种热激蛋白在细胞凋亡信号通路中的调控作用. 中国生物化学与分子生物学报, 2011, 27(1): 22-31.

Wu B, Li Z Y, Yang S C, Zhang D. Regulation of apoptosis signaling pathway by common heat shock proteins. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(1): 22-31. (in Chinese)

[20]Wang Q M, Tu X J, Zhang J H, Chen X B, Rao L Q. Heat stress-induced BBX18 negatively regulates the thermo tolerance in Arabidopsis. Molecular Biology Reports, 2013, 40: 2679-2688.

[21]Sangster T A, Queitsch C. The HSP90 chaperone complex, an emerging force in plant development and phenotypic plasticity. Current Opinion in Plant Biology, 2005, 8: 86-92.

[22]Jagadish S V, Muthurajan R, Oane R, Wheeler T R, Heuer S, Bennett J, Craufurd P Q. Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). Journal of Experimental Botany, 2010, 61:143-156.

[23]Rensink W, Lobst S, Hart A, Stegalkina S, Liu J, Buell C R, Gene expression pro?ling of potato responses to cold, heat, and salt stress. Functional & Integrative Genomics, 2005, 5: 201-207.

[24]Fu S, Meeley R, Scanlon M J. Empty pericarp2 encodes a negative regulator of the heat shock response and is required for maize embryogenesis. The Plant Cell, 2002, 14(12): 3119-3132.

[25]Fu S, Scanlon M J. Clonal mosaic analysis of EMPTY PERICARP2 revealsnonredundant functions of the duplicated HEAT SHOCK FACTOR BINDING PROTEINs during maize shoot development. Genetics, 2004, 167 (3): 1381-1394.

[26]Fu S, Rogowsky P, Nover L, Scanlon M J. The maize heat shock factor-binding protein paralogs EMP2 and HSBP2 interact non-redundantly with specific heat shock factors. Planta, 2006, 224(1): 42-52.
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