Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (7): 1419-1425.doi: 10.3864/j.issn.0578-1752.2013.07.012

• HORTICULTURE • Previous Articles     Next Articles

Isolation of Flowering Locus T Ortholog and the Effects on Blooming of Cymbidium faberi

 SUN  Chong-Bo, XIANG  Lin, LI  Xiao-Bai, QIN  De-Hui, LI  Bo-Jun, GUO  Fang-Qi, WU  Chao   

  1. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021
  • Received:2012-05-15 Online:2013-04-01 Published:2013-01-16

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Abstract: 【Objective】The objective of this study was to analyze the function of Flowering locus T(FT) on blooming in Cymbidium faberi. 【Method】 The full-length cDNA sequence of CfFT was obtained by RT-PCR and RACE cloning technology. The expression patterns of CfFT in different tissues and flower buds of different developmental stages were analyzed by real-time quantitative RT-PCR. To investigate the function of CfFT, an expression vector was constructed for transformation into tobacco by using agrobacterium-mediated method.【Result】In flowers, CfFT was expressed more in young flower buds than in mature flowers and was predominantly expressed in young ovary. Ectopic expression of CfFT in transgenic tobacco plants showed novel phenotypes by flowering earlier than wild-type plants. Real-time quantitative RT-PCR analysis suggested that expression levels of CfFT closely related with the flowering time of different 35S::CfFT transgenic tobacco lines. Further analysis of the flower time related genes indicated that the expression of genes LEAFY (LFY), APETALLA1 (AP1), FRUITFULL (FUL) and SEPALLATA1 (SEP1) were closely related with the expression of FT in 35S::CfFT transgenic tobacco plants.【Conclusion】Ectopic expression of CfFT gene promote flowering in transgenic tobacco.

Key words: Cymbidium faberi , FT gene , flower induction , tobacco , real time quantitative RT-PCR

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