Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 4057-4066.doi: 10.3864/j.issn.0578-1752.2012.19.018

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Distribution and Temporal Expression of Staphylococcus aureus Enterotoxin Genes in Foodborne Staphylococcus aureus Strains

 YANG  Jing, YANG  Jun, HUANG  Ji-Chao, HE  Wei-Ling, ZHANG  Chi, HUANG  Ming   

  1. 1.南京农业大学食品科技学院/教育部肉品加工与质量控制重点实验室，南京 210095
    2.南京市产品质量监督检验院，南京  210028
  • Received:2012-03-01 Online:2012-10-01 Published:2012-08-17

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Abstract: 【Objective】 The objective of this study is to investigate the distribution of different serum enterotoxins in food-borne Staphylococcus aureus and to further analyze their temporal expression of mRNA.【Method】Fifty-one foodborne S. aureus isolates were separated, and the presence of 11 staphylococcal enterotoxins was identified by PCR technology. After the total RNA was extracted, the expression of enterotoxin mRNAs during the cell cycle were assayed in selected isolates by reverse transcriptional real-time PCR with internal control genes ftsZ and ropB. 【Result】 Eight SE genes were detected in the 51 food-borne S. aureus isolates. The most prevalent SE genes were seg and sei. During the growth cycle, the patterns of SE mRNA gene expression were similar in all of the selected isolates. The enterotoxin mRNAs were peaked in the post-exponential growth phase and then rapidly decreased. However, the relative expression level of different enterotoxin genes in the same isolate was different, and the same SE gene's expression level also varied among strains.【Conclusion】The distribution and temporal expression of foodborne enterotoxin genes were studied, which would be valuable to the study of staphylococcal food poisoning (SPF) mechanism and the control of food safety.

Key words: foodborne Staphylococcus aureus, enterotoxin, gene typing, temporal expression, real-time PCR

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