Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3422-3429.doi: 10.3864/j.issn.0578-1752.2012.16.023

• VETERINARY SCIENCE • Previous Articles     Next Articles

Epidemiological Survey and Identification of Theileria Parasite Infection for Small Ruminants in Some Parts of China

 LI  You-Quan, PENG  Yu-率, LIU  Zhi-Jie, GUAN  Gui-Quan, YANG  Ji-Fei, CHEN  Ze, NIU  Qing-Li, LUO  Jian-Xun, YIN  Hong   

  1. 中国农业科学院兰州兽医研究所/家畜疫病病原生物国家重点实验室/甘肃省动物寄生虫病重点实验室，兰州 730046
  • Received:2012-02-07 Online:2012-08-15 Published:2012-07-10

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Abstract:  【Objective】The objective of this experiment is to investigate the current epidemiological status of ovine and caprine theileriosis and identified its pathogens in China. 【Method】The genomic DNA of the blood samples of sheep and goats and ticks were amplified using specific primers to T. uilenbergi, T. luwenshuni and T. ovis, respectively. The 18S rRNA gene sequences from the positive samples were obtained and used for phylogenetical analysis.【Result】On the basis of the PCR results, wide spreading of the theileriosis and significant difference in terms of pathogen species and prevalence in the investigated sites were observed. Co-infection of T. uilenbergi and T. luwenshuni was found only in Gansu Provinces. T. ovis infection was found in Kashi, Xinjiang, but T. luwenshuni infection was detected in Hubei Province but no clinical cases were found. None Theileria infection in Yunnan Province was found. 【Conclusion】The prevalence and infection situation of ovine and caprine theileriosis varied markedly in the studied sites. The data will be useful for guiding the comprehensive prevention and control of ovine and caprine theileriosis in these regions.

Key words: theileriosis, investigation, identification

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