Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3430-3436.doi: 10.3864/j.issn.0578-1752.2012.16.024

• RESEARCH NOTES • Previous Articles     Next Articles

Isolation and Functional Analysis of the Low-Temperature-Responsive Gene MtbHLH-1 in Medicago truncatula

 LI  Yu-Wei, LIAN  Rui-Li, WANG  Xin-Min   

  1. 郑州牧业工程高等专科学校药物工程系，郑州 450011
  • Received:2012-02-10 Online:2012-08-15 Published:2012-06-07

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Abstract: 【Objective】This study is aimed to characterize the low-temperature-responsive gene MtbHLH-1 in M. truncatula by clone and analyse its sequence, and study the function of MtbHLH-1 in low temperature response. 【Method】The full-length cDNA sequence of MtbHLH-1 was isolated by RT-PCR. The obtained cDNA sequence and the deduced amino acid sequence were analysed. Semi-quantitative RT-PCR was used to assess the expression of MtbHLH-1 in response to low temperature (4℃) stress. 【Result】The sequencing result showed that the cloned cDNA (designated as MtbHLH-1, GenBank Accession No. JN800447) was 778 bp, contains an open reading frame (ORF) of 741 bp, and encoding a protein with 246 amino acids. BLASTp search revealed 45% to 73% similarities between the MtbHLH-1 protein and those reported bHLHs from other species, such as Glycine max, Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum and Zea mays. The qRT-PCR result showed that MtbHLH-1 was expressed upregulatedly in response to low temperature stress.【Conclusion】The cold stress response transcription factor MtbHLH-1 was cloned from M. truncatula and the semi-quantitative analysis of the gene MtbHLH-1 under low temperature demonstrate that the MtbHLH-1 gene probably improved low temperature stress tolerant capacities.

Key words: Medicago truncatula, MtbHLH-1, bHLH transcription factors, functional analysis

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